Central pathways mediating autonomic cardiovascular adjustments to fear

• Project/Area Number: 16K15190
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Tottori University
• Principal Investigator: Koba Satoshi 鳥取大学, 医学部, 准教授 (40565743)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 恐怖 / 徐脈 / 自律神経 / 恐怖心 / 副交感神経 / 交感神経 / 光遺伝学 / テレメトリー / すくみ / 中脳中心灰白質 / 視床下部 / 扁桃体 / 免疫染色

Outline of Final Research Achievements

This project aimed to identify central circuitries underlying fear bradycardia. The neural tracing experiments combined with immunofluorescence found several central cardiovascular multisynaptic pathways throughout the rat whole brain. In vivo observations of cardiovascular parameters during fear in rats combined with optogenetics or DREADDs were further conducted to determine functional roles a cardiovascular pathway identified by neural tracing experiments plays in fear bradycardia. Selective inhibition of neuronal excitation by the cardiovascular pathway did not affect fear bradycardia in conscious rats.

Research Products

• [Journal Article] Angiotensin II, oxidative stress, and sympathetic nervous system hyperactivity in heart failure (2018)
  • Author(s): Satoshi Koba
  • Journal Title: Yonago Acta Medica Volume: 印刷中
  • NAID: 130007613079
• [Journal Article] Distribution of Fos-immunoreactive cells in the ventral part of rat medulla following voluntary treadmill exercise (2017)
  • Author(s): Kumada Nao、Koba Satoshi、Hanai Eri、Watanabe Tatsuo
  • Journal Title: Auton Neurosci Volume: 208 Pages: 80-87
  • DOI: 10.1016/j.autneu.2017.09.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Role played by periaqueductal gray neurons in parasympathetically mediated fear bradycardia in conscious rat (2016)
  • Author(s): Koba, S., Inoue, R. & Watanabe, T.
  • Journal Title: Physiological Reports Volume: 4 Issue: 12 Pages: e12831-e12831
  • DOI: 10.14814/phy2.12831
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Central command: brain control of the circulation during exercise (2016)
  • Author(s): 木場智史
  • Journal Title: 自律神経 Volume: 53(2) Pages: 118-121
  • Peer Reviewed
• [Presentation] Central neural circuitries underlying sympathetic adjustments to exercise (2018)
  • Author(s): Satoshi Koba
  • Organizer: 第95回日本生理学会大会
  • Invited
• [Presentation] Are PVN‐RVLM neurons involved in sympathetic dysfunction in heart failure? (2018)
  • Author(s): Eri Hanai, Satoshi Koba, Nao Kumada, Tatsuo Watanabe
  • Organizer: 第95回日本生理学会大会
• [Presentation] Central neural mechanisms underlying sympathetic hyperactivity in heart failure (2018)
  • Author(s): Satoshi Koba
  • Organizer: 第95回日本生理学会大会
  • Invited
• [Presentation] Blood pressure regulation by neurons projecting from the mesencephalic locomotor region to the rostral ventrolateral medulla in rats (2018)
  • Author(s): Nao Kumada, Satoshi Koba, Eri Hanai, Naoya Kataoka, Kazuhiro Nakamura, Tatsuo Watanabe
  • Organizer: 第95回日本生理学会大会
• [Presentation] Posterior hypothalamic neurons projecting to the hypothalamic paraventricular nucleus are activated by exercise in rats (2017)
  • Author(s): Eri Hanai, Satoshi Koba, Tatsuo Watanabe
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松(静岡県浜松市)
  • Year and Date: 2017-03-28
• [Presentation] Distribution of activated catecholaminergic neurons by exercise in the rat ventral medulla (2017)
  • Author(s): Nao Kumada, Satoshi Koba, Tatsuo Watanabe
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松(静岡県浜松市)
  • Year and Date: 2017-03-28
• [Presentation] 運動時交感神経活性の中枢経路の探索 (2017)
  • Author(s): 花井映里、木場智史、熊田奈桜、渡邊達生
  • Organizer: 第12回環境生理学プレコングレス
  • Place of Presentation: サーラシティ浜松(静岡県浜松市)
  • Year and Date: 2017-03-27
• [Presentation] 中脳歩行誘発野から延髄吻側腹外側野への投射神経刺激による昇圧反応 (2017)
  • Author(s): 熊田奈桜、木場智史、花井映里、渡邊達生
  • Organizer: 第69回日本生理学会中国四国地方会
• [Presentation] Effect of optogenetic inhibition of PVN-RVLM neurons on sympathetic nerve activity in anesthetized rats with heart failure (2017)
  • Author(s): Eri Hanai, Satoshi Koba, Nao Kumada, Tatsuo Watanabe
  • Organizer: ISAN 2017 - International Society for Autonomic Neuroscience
  • Int'l Joint Research
• [Presentation] Effect of glutamate receptor blockade in RVLM on PVN stimulation-elicited sympathoexcitation in anesthetized rats (2017)
  • Author(s): Satoshi Koba, Eri Hanai, Nao Kumada, Naoya Kataoka, Kazuhiro Nakamura, Tatsuo Watanabe
  • Organizer: ISAN 2017 - International Society for Autonomic Neuroscience
  • Int'l Joint Research
• [Presentation] Optogenetic stimulation of PVN-RVLM neurons increases sympathetic nerve activity in anesthetized rats (2017)
  • Author(s): Satoshi Koba, Eri Hanai, Nao Kumada, Naoya Kataoka, Kazuhiro Nakamura, Tatsuo Watanabe
  • Organizer: Experimental Biology 2017
  • Int'l Joint Research
• [Presentation] 床下部後部から視床下部室傍核への中枢経路は 運動によって活性化する (2016)
  • Author(s): 花井映里、木場智史、渡邊達生
  • Organizer: 第68回日本生理学会中国四国地方会
  • Place of Presentation: 岡山大学Junko Fukutakeホール(岡山県岡山市)
  • Year and Date: 2016-11-05
• [Presentation] 運動トレーニングと循環中枢回路 (2016)
  • Author(s): 木場智史、花井映里、熊田奈桜
  • Organizer: SICEライフエンジニアリング部門シンポジウム
  • Place of Presentation: 大阪国際交流センター(大阪府大阪市)
  • Year and Date: 2016-11-03
  • Invited
• [Presentation] Central projections to medulla for autonomic adjustments to exercise in rats (2016)
  • Author(s): Satoshi Koba, Momone Kato, Eri Hanai, Tatsuo Watanabe
  • Organizer: Experimental Biology 2017
  • Place of Presentation: San Diego Convention Center (米国サンディエゴ)
  • Year and Date: 2016-04-02
  • Int'l Joint Research