Development of a new transgenic mouse model as a platform for the imaging-based drug discovery and development
| Project/Area Number |
16K15201
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 教道 名古屋大学, 医学部附属病院, 特任助教 (30726310)
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| Co-Investigator(Renkei-kenkyūsha) |
NAGAI Taku 名古屋大学, 医学部附属病院, 准教授 (10377426)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | イメージング創薬 / Npas4 / トランスジェニックマウス / Homer1a / キンドリング / 動物モデル / 神経科学 / 脳神経疾患 |
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| Outline of Final Research Achievements |
In 2016, to regulate the expression of target genes in a neuronal activity-dependent manner, we created a new transgenic mice line (NPAS4-tTA mice) in which the expression of tTA gene is regulated under the control of Npas4 promoter. We obtained the double transgenic mice following mating with tet0-ChR2-EYFP transgenic mice as a reporter. The double transgenic mice exhibited a high background expression of EYFP in the brain with or without pentylentetrazole (PTZ) treatment.
In 2017, we prepared cyclic AMP response element (CRE)-luciferase expression system using AAV vector. We observed a significant increase in chemiluminescent after cocaine treatment in vivo in the brain of mice expressing the CRE-luciferase gene. As to the functional analysis of target genes of Npas4, we demonstrated that Npas4-Homer1a signal played a role in the regulation of homeostatic scaling in the hippocampus.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.2018
Author(s)
A Sobue, N Ito, T Nagai, W Shan, K Hada, Nakajima A, Y Murakami, A Mouri, Y Yamamoto, T Nabeshima, K Saito, K Yamada
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Journal Title
Glia
Volume: 66
Issue: 5
Pages: 1034-52
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Valocin-containing protein (VCP) is a novel IQ motif containing GTPase activating protein 1 (IQGAP1) interacting protein.2017
Author(s)
Itoh, N., Nagai, T,, Watanabe, T., Taki, K., Nabeshima, N., Kaibuchi, K., Yamada, K.
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Journal Title
Biochem Biophys Res Commun.
Volume: 493
Issue: 4
Pages: 1384-1389
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Protective potential of the glutathione peroxidase-1 gene in abnormal behaviors induced by phencyclidine in mice.2016
Author(s)
Tran TV, Shin EJ, Jeong JH, Lee JW, Lee Y, Jang CG, Nah SY, Lei XG, Toriumi K, Yamada K, Nabeshima T, Kim HC.
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Journal Title
Mol Neurobiol.
Volume: -
Issue: 9
Pages: 7042-7062
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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