Development of magnetic antibiotics

• Project/Area Number: 16K15205
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Yokohama City University
• Principal Investigator: ISHIKAWA Yoshihiro 横浜市立大学, 医学研究科, 教授 (40305470)
• Co-Investigator(Kenkyū-buntansha): 垣内 史敏 慶應義塾大学, 理工学部(矢上), 教授 (70252591)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 抗生剤 / 磁性 / ドラッグデリバリー / 薬理学 / 微生物

Outline of Final Research Achievements

The method for evaluating metal material has been applied to pharmaceutical compounds. Accordingly, we have found compounds with strong magnetism, which also has toxicity. With chemical researchers, we have synthesized magnetic compounds with less toxicity. In this research project, we have examined this compound and studied its application for magnetizing antibiotic compounds. We have synthesized ampicillin which has been magnetized by this compounds. We have found that this magnetized ampicillin was indeed weakly magnetic. Our findings suggest that it is feasible to magnetize antibiotics. In the future study it will be needed to evaluate antibiotic activity of magnetized ampicillin and its guided delivery by magnet.

Research Products

• [Journal Article] The role of Epac in the heart (2017)
  • Author(s): T. Fujita, M. Umemura, U. Yokoyama, S. Okumura, and Y. Ishikawa
  • Journal Title: Cellular and Molecular Life Sciences Volume: 74 Issue: 4 Pages: 591-606
  • DOI: 10.1007/s00018-016-2336-5
  • Peer Reviewed
• [Journal Article] Arterial graft with elastic layer structure grown from cells. (2017)
  • Author(s): Yokoyama U, Tonooka Y, Koretake R, Akimoto T, Gonda Y, Saito J, Umemura M, Fujita T, Sakuma S, Arai F, Kaneko M, Ishikawa Y.
  • Journal Title: Sci Rep Volume: 1 Issue: 1 Pages: 140-140
  • DOI: 10.1038/s41598-017-00237-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Magnetic metal complex-conducting copolymer core-shell nanoassemblies for single-drug anticancer platform. (2017)
  • Author(s): Kim J-H, Eguchi H,Umemura M, Sato I, Yamada S, Hoshino Y, Masuda T, Aoki I, Sakurai,YamamotoM, and Ishikawa YK,
  • Journal Title: NPG Asia Materials. Volume: 9 Issue: 3 Pages: e367-e367
  • DOI: 10.1038/am.2017.29
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hyperthermia and chemotherapy using Fe(Salen) nanoparticles might impact glioblastoma treatment. (2017)
  • Author(s): Ohtake M, Umemura M, Sato I, Akimoto T, Oda K, Nagasako A, Kim JH, Fujita T, Yokoyama U, Nakayama T, Hoshino Y, Ishiba M, Tokura S, Hara M, Muramoto T, Yamada S, Masuda T, Aoki I, Takemura Y, Murata H, Eguchi H, Kawahara N, Ishikawa Y.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 42783-42783
  • DOI: 10.1038/srep42783
  • Peer Reviewed / Open Access
• [Journal Article] Pathology and Molecular Mechanisms of Coarctation of the Aorta and its Association with the Ductus Arteriosus. (2017)
  • Author(s): Yokoyama U, Ichikawa Y, Minamisawa S, Ishikawa Y
  • Journal Title: J Physiol Sci. Volume: 67 Issue: 2 Pages: 259-270
  • DOI: 10.1007/s12576-016-0512-x
  • NAID: 40021113914
  • Peer Reviewed / Open Access
• [Journal Article] Use of Three-dimensional Arterial Models To Predict the In Vivo Behavior of Nanoparticles for Drug Delivery (2016)
  • Author(s): P. Chetprayoon, M. Matsusaki, U. Yokoyama, T. Tejima, Y. Ishikawa, M. Akashi
  • Journal Title: Angew. Chem. Int. Ed. Volume: 55 Issue: 14 Pages: 4461-4466
  • DOI: 10.1002/anie.201509752
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Simultaneous hyperthermia-chemotherapy with controlled drug delivery using single-drug nanoparticles (2016)
  • Author(s): Itaru Sato, Masanari Umemura, Kenji Mitsudo, Hidenobu Fukumura, Jeong-Hwan Kim, Yujiro Hoshino Hideyuki Nakashima, Mitomu Kioi, Rina Nakakaji, Motohiko Sato, Takayuki Fujita, Utako Yokoyama, Satoshi Okumura, Hisashi Oshiro, Haruki Eguchi, Iwai Tohnai and Yoshihiro Ishikawa.
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 24629-24629
  • DOI: 10.1038/srep24629
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disruption of Epac1 protects the heart from adenylyl cyclase type 5-mediated cardiac dysfunction． (2016)
  • Author(s): Cai W，Fujita T，Hidaka Y，Jin H，Suita K，Prajapati R，Liang C，Umemura M，Yokoyama U，Sato M，Okumura S，Ishikawa Y
  • Journal Title: Biochem Biophys Res Commun. Volume: 475 Issue: 1 Pages: 1-7
  • DOI: 10.1016/j.bbrc.2016.04.123
  • Peer Reviewed
• [Journal Article] Transient receptor potential cation 3 channel regulates melanoma proliferation and migration． (2016)
  • Author(s): Oda K，Umemura M，Nakakaji R，Tanaka R，Sato I，Nagasako A，Oyamada C，Baljinnyam E，Katsumata M，Xie L，Narikawa M，Yamaguchi Y，Akimoto T，Ohtake M，Fujita T，Yokoyama U，Iwatsubo K，Aihara M and Ishikawa Y
  • Journal Title: J Physiol Sci. Volume: 該当なし Issue: 4 Pages: 497-505
  • DOI: 10.1007/s12576-016-0480-1
  • NAID: 40021238419
  • Peer Reviewed / Open Access
• [Journal Article] Glutamate Promotes Contraction of the Rat Ductus Arteriosus (2016)
  • Author(s): S. Fujita, U. Yokoyama, R. Ishiwata, R. Aoki, K. Nagao, D. Masukawa, M. Umemura, T. Fujita, S. Iwasaki, S. Nishimaki, K. Seki, S. Ito, Y. Goshima, T. Asou, M. Masuda, and Y. Ishikawa
  • Journal Title: Circulation Journal Volume: 80 Issue: 11 Pages: 2388-2396
  • DOI: 10.1253/circj.CJ-16-0649
  • NAID: 130005433144
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Vidarabine, an Anti-Herpes Virus Agent, Protects Against the Development of Heart Failure With Relatively Mild Side-Effects on Cardiac Function in a Canine Model of Pacing-Induced Dilated Cardiomyopathy (2016)
  • Author(s): T. Nakamura, T. Fujita, M. Kishimura, K. Suita, Y. Hidaka, W. Cai, M. Umemura, U. Yokoyama, M. Uechi, and Y. Ishikawa
  • Journal Title: Circulation Journal Volume: 80 Issue: 12 Pages: 2496-2505
  • DOI: 10.1253/circj.CJ-16-0736
  • NAID: 130005284971
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] Role of phosphodiesterase 4 expression in the epac1 signaling-dependent skeletal muscle hypertrophic action of clenbuterol． (2016)
  • Author(s): Ohnuki Y，Umeki D，Mototani Y，Shiozawa K，Nariyama M，Ito A，Kawamura N，Yagisawa Y，Jin H，Cai W，Suita K，Saeki Y，Fujita T，Ishikawa Y，Okumura S.
  • Journal Title: Physiological reports. Volume: 4 Issue: 10 Pages: e12791-e12791
  • DOI: 10.14814/phy2.12791
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epac activation inhibits IL-6-induced cardiac myocyte dysfunction. (2016)
  • Author(s): Jin H，Fujita T，Jin M，Kurotani R，Hidaka Y，Cai W，Suita K，Prajapati R，Liang C，Ohnuki Y，Mototani Y，Umemura M，Yokoyama U，Sato M，Okumura S，Ishikawa Y.
  • Journal Title: J Physiol Sci. Volume: 印刷中 Issue: 1 Pages: 77-87
  • DOI: 10.1007/s12576-016-0509-5
  • NAID: 40021443658
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Heterozygous deletion of sarcolipin maintains normal cardiac function. (2015)
  • Author(s): Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, Jin M, Yokota T, Ishikawa Y, Nakano A, Goda N, Minamisawa S.
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 310(1) Issue: 1 Pages: 92-103
  • DOI: 10.1152/ajpheart.00411.2015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 心筋細胞の生存維持におけるTCTPの役割. (2017)
  • Author(s): 藤田 孝之，蔡 文倩，日高 祐子，金 慧玲，プラジャパティ ラジェッシュ，梁 宸，中村 隆，石川 義弘.
  • Organizer: 第94回日本生理学会大会.
  • Place of Presentation: アクトシティ浜松 (愛知県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] The function of Transient Receptor Potential Canonical 3 (TRPC3) Channel in cell proliferation. (2017)
  • Author(s): Umemura M, Oda K, Ishikawa Y.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan.
  • Place of Presentation: アクトシティ浜松 (愛知県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] Human gene analysis identified tissue plasminogen activator as a mediator of disrupting the internal elastic lamina in the ductus arteriosus. (2017)
  • Author(s): Saito J, Yokoyama U, Ishikawa Y.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan.
  • Place of Presentation: アクトシティ浜松 (愛知県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] 心臓型アデニル酸シクラーゼの阻害は心機能を低下させることなくカテコラミン誘発性不整脈を抑制する. (2017)
  • Author(s): 吹田 憲治，藤田 孝之，奥村 敏，石川 義弘.
  • Organizer: 第94回日本生理学会大会.
  • Place of Presentation: アクトシティ浜松 (愛知県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] An EP4 antagonist has inhibitory and therapeutic effects on mouse models of abdominal aortic aneurysm. (2017)
  • Author(s): Al Mamun,Yokoyama U, Ishiwata R, Isono O, Nagabukuro H, Ishikawa Y.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan.
  • Place of Presentation: アクトシティ浜松 (愛知県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] In vitro fabrication of functional arterial graft by supraphysiological hydrostatic pressurization. (2017)
  • Author(s): Yokoyama U, Saito J, Sakuma S, Arai F, Kaneko M, Ishikawa Y.
  • Organizer: The 81th Annual Scientific Meeting of the Japanese Circulation Society.
  • Place of Presentation: 金沢県立音楽堂
  • Year and Date: 2017-03-17
• [Presentation] ON-CHIP CELL GYM. (2017)
  • Author(s): Horade M, Kaneko M, Tsai CHD, Ito H, Higashino N, Akai T, Yokoyama U, Ishikawa Y, Sakuma S, Arai F.
  • Organizer: The 30th IEEE International Conference on Micro Electro Mechanical Systems.
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2017-01-22
  • Int'l Joint Research
• [Presentation] Transient receptor potential cation channel 3 (TRPC3) regulates tumor proliferation and migration of BRAF wild type human malignant melanoma． (2016)
  • Author(s): Oda K，Umemura M，Sato I，Nagasako A，Oyamada C，Katsumata M，Nakakagi R，Narikawa M，Akimoto T，Ohtake M，Yamaguchi Y，Aihara M，Ishikawa Y
  • Organizer: 日本研究皮膚科学，第41回年次学術大会・総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-12-09
• [Presentation] Fibulin-1 は平滑筋細胞遊走を介して動脈管内膜肥厚を誘導する． (2016)
  • Author(s): 伊藤智子，横山詩子，益田宗孝，麻生俊英，石川義弘.
  • Organizer: 第61回日本新生児成育医学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2016-12-01
• [Presentation] EP4 signaling in smooth muscle cells attracts inflammatory immune responses in the aorta. (2016)
  • Author(s): Ishiwata R, Yokoyama U, Ichikawa Y, Kurotaki D, Yasuda S, Goda M, Suzuki S, Masuda M, Tamura T, Ishikawa Y
  • Organizer: The 89th Scientific Session, American Heart Association (AHA).
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Human Gene Profiling Reveals contribution of Tissue Plasminogen Activator to Intimal Thickening of the Ductus Arteriosus. (2016)
  • Author(s): Saito J, Yokoyama U, Masuda M, Asou T, Ishikawa Y
  • Organizer: The 89th Scientific Session, American Heart Association (AHA).
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Fibulin-1 plays a role in smooth muscle cell migration and intimal thickening in the ductus arteriosus. (2016)
  • Author(s): Ito S, Yokoyama U, Yanai C, Masuda M, Asou T, Ishikawa Y:
  • Organizer: The 89th Scientific Session, American Heart Association (AHA).
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] 組織型プラスミノーゲン活性化因子による動脈管内弾性板の分解 (2016)
  • Author(s): 齋藤純一, 横山詩子, 石川義弘.
  • Organizer: 第15回日本心臓血管発生研究会
  • Place of Presentation: 国立循環器病センター（大阪府吹田市）
  • Year and Date: 2016-10-14
• [Presentation] PGE2-EP4シグナルはfibulin-1を介して動脈管平滑筋遊走を促進する. (2016)
  • Author(s): 伊藤智子, 横山詩子, 石川義弘.
  • Organizer: 第15回日本心臓血管発生研究会
  • Place of Presentation: 国立循環器病センター（大阪府吹田市）
  • Year and Date: 2016-10-14
• [Presentation] Anticancer Nanomagnet-loaded Smart Nano-Ensembles for Magneto-Drug Delivery，MRI，and Hyperthermal Cancer Targeting． (2016)
  • Author(s): Kim JH, Eguchi H, Umemura M, Ishikawa Y.
  • Organizer: Asia Nano.
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-10-10
  • Int'l Joint Research
• [Presentation] An application of a novel intrinsic magnetized paclitaxel for oral cancer． (2016)
  • Author(s): Nakakaji R，Umemura M，Ohtake M，Oda K，Kioi M，Eguchi H，Tohnai I，Ishikawa Y.
  • Organizer: 第75回日本癌学会学術大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 神経膠芽腫に対する有機磁性体化合物を用いた化学温熱同時療法． (2016)
  • Author(s): 大竹 誠，梅村将就，秋本大輔，江口晴樹，石川義弘，川原信隆.
  • Organizer: 第75回日本脳神経外科学術集会総会
  • Place of Presentation: 福岡国際会議場、福岡サンパレス、マリンメッセ福岡（福岡県福岡市）
  • Year and Date: 2016-09-29
• [Presentation] Exchange protein activated by cyclic AMP 1 (Epac1) promotes bFGF-induced arterial smooth muscle cell migration. (2016)
  • Author(s): Kato Y, Yokoyama U, Kubota T, Ishikawa Y.
  • Organizer: The 32nd World Congress of Biomedical Laboratory Science.
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2016-08-31
  • Int'l Joint Research
• [Presentation] Functional arterial graft fabricated from pressurized cell-layers． (2016)
  • Author(s): Ishikawa Y, Akimoto T, Saito J, Gonda Y, Sakuma S, Arai F, Kaneko M, Yokoyama U．
  • Organizer: European Society of Cardiology
  • Place of Presentation: Rome, Italy
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] TCTP down-regulation-induced cardiac cell death through BNIP3 may be a pivotal mechanism of Doxorubicin-induced heart failure． (2016)
  • Author(s): Cai W，Fujita T，Hidaka Y，Jin H，Suita K，Hasegawa N，Kubota K，Liang C，Ishikawa Y
  • Organizer: European Society of Cardiology
  • Place of Presentation: Rome, Italy
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] ヒト動脈管における内膜肥厚部の遺伝子プロファイリング． (2016)
  • Author(s): 齋藤純一，横山詩子，麻生俊英，石川義弘.
  • Organizer: 第52回日本周産期・新生児医学会学術集会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-07-16
• [Presentation] In-vitro fabrication of scaffold-free arterial grafts by periodic hydrodynamic pressurization. (2016)
  • Author(s): Yokoyama U, Tonooka Y, Koretake R, Akimoto T, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: International and Interdisciplinary Symposium 2016 “Towards a New Era of Cardiovascular Research
  • Place of Presentation: 東京医科歯科大学（東京都文京区）
  • Year and Date: 2016-07-11
  • Int'l Joint Research
• [Presentation] 平滑筋細胞におけるPGE2-EP4シグナルは腹部大動脈瘤の発症を促す． (2016)
  • Author(s): 石渡 遼，横山詩子，市川泰弘，黒滝大翼，田村智彦，石川義弘.
  • Organizer: 第37回日本循環制御医学会総会.
  • Place of Presentation: ステーションコンファレンス東京（東京都千代田区）
  • Year and Date: 2016-07-07
• [Presentation] キソルビシン誘発心不全発症における，p53を介したtranslationally controlled tumor protein（TCTP）発現抑制の役割． (2016)
  • Author(s): 蔡 文倩，藤田孝之，日高祐子，金 慧玲，Rajesh Prajapati，梁 宸，石川義弘.
  • Organizer: 第37回循環制御医学会総会
  • Place of Presentation: ステーションコンファレンス東京（東京都千代田区）
  • Year and Date: 2016-07-07
• [Presentation] The role of Epac in the development of atrial fibrillation in mice． (2016)
  • Author(s): Rajesh Prajapati，藤田孝之，蔡 文倩，吹田憲治，長谷川希望，金 慧玲，梁 晨，日高祐子，石川義弘.
  • Organizer: 第37回日本循環制御医学会総会
  • Place of Presentation: ステーションコンファレンス東京（東京都千代田区）
  • Year and Date: 2016-07-07
• [Presentation] 敗血症性心不全における心機能低下に対するEpac1の保護的役割． (2016)
  • Author(s): 金 慧玲，藤田孝之，金 美花，蔡 文倩，日高祐子，吹田憲治，Rajesh Prajapati，梁 晨，奥村 敏，石川義弘
  • Organizer: 第37回日本循環制御医学会総会
  • Place of Presentation: ステーションコンファレンス東京（東京都千代田区）
  • Year and Date: 2016-07-07
• [Presentation] The behavior of cells during Cell Exercise. (2016)
  • Author(s): Higashino N, Kaneko M, Yokoyama U, Ishikawa Y.
  • Organizer: ROBOMECH2016
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-08
• [Remarks] http://www-user.yokohama-cu.ac.jp/~seiri1/
• [Patent(Industrial Property Rights)] 磁性高分子ミセル (2016)
  • Inventor(s): J-H.Kim, Ishikawa Y, Umemura M.
  • Industrial Property Rights Holder: 横浜市立大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-05-24
• [Patent(Industrial Property Rights)] がん治療装置 (2016)
  • Inventor(s): 石川義弘、梅村将就、秋本大輔
  • Industrial Property Rights Holder: 横浜市立大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-228164
  • Filing Date: 2016-11-24