Study of the mechanism of biosynthesis of polysulfurated proteins coupled with translation

• Project/Area Number: 16K15208
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: AKAIKE Takaaki 東北大学, 医学系研究科, 教授 (20231798)
• Co-Investigator(Kenkyū-buntansha): 藤井 重元 東北大学, 医学系研究科, 准教授 (00325333) ; 井田 智章 東北大学, 医学系研究科, 助教 (70570406)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: タンパク質ポリサルファ化 / システインポリスルフィド / システイン-tRNA合成酵素 / 活性イオウ分子種

Outline of Final Research Achievements

Polysulfur is reactive persulfide species which have extra sulfur atoms in cysteine thiol and cellular proteins contain polysulfur abundantly (protein polysulfuration). In this study, we investigated the translation-coupled mechanism of protein polysulfuration mediated by cysteinyl-tRNA synthetase (CARS) and its biological functions. CARSs from various biological species including bacteria and mammals are found to have cysteine persulfide-producing activity and to be critically involved in protein polysulfuration. Protein polysulfuration is suggested to play important roles in the regulation of cellular function such as mitochondrial morphogenesis.

Research Products

• [Int'l Joint Research] National Institute of Oncology(Hungary)
• [Int'l Joint Research] University of Southampton(英国)
• [Int'l Joint Research] Sonoma State University/University of Vermont(米国)
• [Int'l Joint Research] German Cancer Research Center(Germany)
• [Int'l Joint Research] Sonoma State University/Johns Hopkins University/University of Alabama at Birmingham(米国)
• [Journal Article] Involvement of nitric oxide/reactive oxygen species signaling via 8-nitro-cGMP formation in 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in PC12 cells and rat cerebellar granule neurons (2018)
  • Author(s): Masuda Kumiko、Tsutsuki Hiroyasu、Kasamatsu Shingo、Ida Tomoaki、Takata Tsuyoshi、Sugiura Kikuya、Nishida Motohiro、Watanabe Yasuo、Sawa Tomohiro、Akaike Takaaki、Ihara Hideshi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 3 Pages: 2165-2170
  • DOI: 10.1016/j.bbrc.2017.12.088
  • Peer Reviewed / Open Access
• [Journal Article] 8-Nitro-cGMP is a promoter of osteoclast differentiation induced by RANKL (2018)
  • Author(s): Kaneko K.、Miyamoto Y.、Tsukuura R.、Sasa K.、Akaike T.、Fujii S.、Yoshimura K.、Nagayama K.、Hoshino M.、Inoue S.、Maki K.、Baba K.、Chikazu D.、Kamijo R.
  • Journal Title: Nitric Oxide Volume: 72 Pages: 46-51
  • DOI: 10.1016/j.niox.2017.11.006
  • Peer Reviewed / Open Access
• [Journal Article] Important role of endothelial caveolin-1 in the protective role of endothelium-dependent hyperpolarization against nitric oxide-mediated nitrative stress in microcirculation in mice (2018)
  • Author(s): Saito H, Godo S, Sato S, Ito A, Ikumi Y, Tanaka S, Ida T, Fujii S, Akaike T, Shimokawa H.
  • Journal Title: Journal of Cardiovascular Pharmacology Volume: 71 Issue: 2 Pages: 113-126
  • DOI: 10.1097/fjc.0000000000000552
  • Peer Reviewed / Open Access
• [Journal Article] 8-Nitro-cGMP attenuates the interaction between SNARE complex and complexin through S-guanylation of SNAP-25 (2018)
  • Author(s): Kishimoto Y, Kunieda K, Kitamura A, Kakihana Y, Akaike T, Ihara H.
  • Journal Title: ACS Chem Neurosci Volume: 9 Issue: 2 Pages: 217-223
  • DOI: 10.1021/acschemneuro.7b00363
  • Peer Reviewed / Open Access
• [Journal Article] Cysteine perthiosulfenic acid (Cys-SSOH): A novel intermediate in thiol-based redox signaling? (2018)
  • Author(s): Heppner DE, Hristova M, Ida T, Mijuskovic A, Dustin CM, Bogdandi V, Fukuto JM, Dick TP, Nagy P, Li J, Akaike T, van der Vliet A.
  • Journal Title: Redox Biol. Volume: 14 Pages: 379-385
  • DOI: 10.1016/j.redox.2017.10.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Persulfide synthases function coupled with translation and mediate sulfur respiration (2018)
  • Author(s): Fujii S, Sawa T, Motohashi H, Akaike T.
  • Journal Title: Br J Pharmacol Volume: -
  • Peer Reviewed
• [Journal Article] 概論 レドックス疾患学：レドックス制御の破綻による病態と新たな疾患概念 (2018)
  • Author(s): 本橋ほづみ、赤池孝章、内田浩二、末松 誠.
  • Journal Title: 実験医学増刊「レドックス疾患学」 Volume: 36 Pages: 642-648
• [Journal Article] 硫黄呼吸の発見：ペルスルフィド産生酵素による新しいエネルギー代謝 (2018)
  • Author(s): 西村 明、赤池孝章.
  • Journal Title: 現代化学 Volume: 565 Pages: 55-59
  • NAID: 40021493631
• [Journal Article] Synthesis of L-cysteine derivatives containing stable sulfur isotopes and application of this synthesis to reactive sulfur metabolome (2017)
  • Author(s): Ono K, Jung M, Zhang T, Tsutsuki H, Sezaki H, Ihara H, Wei FY, Tomizawa K, Akaike T, Sawa T.
  • Journal Title: Free Radic Biol Med Volume: 106 Pages: 69-79
  • DOI: 10.1016/j.freeradbiomed.2017.02.023
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Quantitative determination of polysulfide in albumins, plasma proteins and biological fluid samples using a novel combined assays approach (2017)
  • Author(s): keda, M. Ishima, Y. Shibata, A. Chuang, V. T. G. Sawa, T. Ihara, H. Watanabe, H. Xian, M. Ouchi, Y. Shimizu, T. Ando, H. Ukawa, M. Ishida, T. Akaike, T. Otagiri, M. Maruyama, T.
  • Journal Title: Analytica Chimica Acta Volume: 969 Pages: 18-25
  • DOI: 10.1016/j.aca.2017.03.027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reactive sulfur species inactivate Ca2+/calmodulin-dependent protein kinase IV via S-polysulfidation of its active-site cysteine residue (2017)
  • Author(s): Takata T, Ihara H, Hatano N, Tsuchiya Y, Akaike T, Watanabe Y.
  • Journal Title: Biochem J Volume: 474 Issue: 15 Pages: 2547-2562
  • DOI: 10.1042/bcj20170092
  • Peer Reviewed
• [Journal Article] Redox regulation of electrophilic signaling by reactive persulfides in cardiac cells (2017)
  • Author(s): Nishida M, Nishimura A, Matsunaga T, Motohashi H, Kasamatsu S, Akaike T.
  • Journal Title: Free Radic Biol Med. Volume: 印刷中 Pages: 30033-3
  • DOI: 10.1016/j.freeradbiomed.2017.01.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disruption of the structural and functional features of surfactant protein A by acrolein in cigarette smoke (2017)
  • Author(s): Takamiya Rina、Uchida Koji、Shibata Takahiro、Maeno Toshitaka、Kato Masaki、Yamaguchi Yoshiki、Ariki Shigeru、Hasegawa Yoshihiro、Saito Atsushi、Miwa Soichi、Takahashi Hiroki、Akaike Takaaki、Kuroki Yoshio、Takahashi Motoko
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 8304-8304
  • DOI: 10.1038/s41598-017-08588-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 8-Nitro-cGMP promotes bone growth through expansion of growth plate cartilage (2017)
  • Author(s): Hoshino Marie、Kaneko Kotaro、Miyamoto Yoichi、Yoshimura Kentaro、Suzuki Dai、Akaike Takaaki、Sawa Tomohiro、Ida Tomoaki、Fujii Shigemoto、Ihara Hideshi、Tanaka Junichi、Tsukuura Risa、Chikazu Daichi、Mishima Kenji、Baba Kazuyoshi、Kamijo Ryutaro
  • Journal Title: Free Radic Biol Med Volume: 110 Pages: 63-71
  • DOI: 10.1016/j.freeradbiomed.2017.05.022
  • Peer Reviewed / Open Access
• [Journal Article] Exposure to Electrophiles Impairs Reactive Persulfide-Dependent Redox Signaling in Neuronal Cells (2017)
  • Author(s): Ihara Hideshi、Kasamatsu Shingo、Kitamura Atsushi、Nishimura Akira、Tsutsuki Hiroyasu、Ida Tomoaki、Ishizaki Kento、Toyama Takashi、Yoshida Eiko、Abdul Hamid Hisyam、Jung Minkyung、Matsunaga Tetsuro、Fujii Shigemoto、Sawa Tomohiro、Nishida Motohiro、Kumagai Yoshito、Akaike Takaaki
  • Journal Title: Chem. Res. Toxicol Volume: 30 Issue: 9 Pages: 1673-1684
  • DOI: 10.1021/acs.chemrestox.7b00120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemical biology of hydropersulfides and related species: Possible roles in cellular protection and redox signaling (2017)
  • Author(s): Alvarez L, Bianco CL, Toscano JP, Lin J. Akaike T, Fukuto JM.
  • Journal Title: Antioxid Redox Signal Volume: 27 Issue: 10 Pages: 622-633
  • DOI: 10.1089/ars.2017.7081
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diversity and microevolution of CRISPR loci in Helicobacter cinaedi (2017)
  • Author(s): Tomida J, Morita Y, Shibayama K, Kikuchi K, Sawa T, Akaike T, Kawamura Y.
  • Journal Title: PLoS One Volume: 12 Issue: 10 Pages: e0186241-e0186241
  • DOI: 10.1371/journal.pone.0186241
  • Peer Reviewed / Open Access
• [Journal Article] Production of reactive persulfide species in chronic obstructive pulmonary disease (2017)
  • Author(s): Numakura T, Sugiura H, Akaike T, Ida T, Fujii S, Koarai A, Yamada M, Onodera K, Hashimoto Y, Tanaka R, Sato K, Shishikura Y, Hirano T, Yanagisawa S, Fujino N, Okazaki T, Tamada T, Hoshikawa Y, Okada Y, Ichinose M
  • Journal Title: Thorax Volume: 72 Issue: 12 Pages: 1074-1083
  • DOI: 10.1136/thoraxjnl-2016-209359
  • Peer Reviewed / Open Access
• [Journal Article] Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics (2017)
  • Author(s): Akaike Takaaki、、、Nishida Motohiro（4）、、、、、、Fujii Shigemoto（20）、、、Motohashi Hozumi（26）
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1177-1177
  • DOI: 10.1038/s41467-017-01311-y
  • NAID: 120007134656
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ニトロ化環状ヌクレオチドと活性イオウ分子によるレドックスシグナル制御と生理機能 (2017)
  • Author(s): 藤井重元、赤池孝章.
  • Journal Title: 化学療法の領域 Volume: 33 Pages: 2194-2195
• [Journal Article] 活性イオウ分子：システインパースルフィドの新規生合成経路の発見とその生理機能 (2017)
  • Author(s): 西村 明、赤池孝章.
  • Journal Title: 硫酸と工業 Volume: 70 (11) Pages: 1-9
  • NAID: 40021404464
• [Journal Article] Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion. (2017)
  • Author(s): Takahashi N, Wei FY, Watanabe S, Hirayama M, Ohuchi Y, Fujimura A, Kaitsuka T, Ishii I, Sawa T, Nakayama H, Akaike T, Tomizawa K.
  • Journal Title: Nucleic Acids Res. Volume: 45 Issue: 1 Pages: 435-445
  • DOI: 10.1093/nar/gkw745
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metabolomic profiling of reactive persulfides and polysulfides in the aqueous and vitreous humors. (2017)
  • Author(s): Kunikata H, Ida T, Sato K, Aizawa N, Sawa T, Tawarayama H, Murayama N, Fujii S, Akaike T, Nakazawa T.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 41984-41984
  • DOI: 10.1038/srep41984
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 1,4-Naphthoquinone activates the HSP90/HSF1 pathway through the S-arylation of HSP90 in A431 cells: Negative regulation of the redox signal transduction pathway by persulfides/polysulfides (2017)
  • Author(s): Abiko Y, Sha L, Shinkai Y, Unoki T, Luong NC, Tsuchiya Y, Watanabe Y, Hirose R, Akaike T, Kumagai Y.
  • Journal Title: Free Radic Biol Med Volume: 104 Pages: 118-128
  • DOI: 10.1016/j.freeradbiomed.2016.12.047
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Synthesis and Characterization of 8-Nitroguanosine 3′,5′-Cyclic Monophosphorothioate Rp-Isomer as a Potent Inhibitor of Protein Kinase G1α (2017)
  • Author(s): Ahmed KA, Zhang T, Ono K, Tsutsuki H, Ida T, Akashi S, Miyata K, Oike Y, Akaike T, Sawa T.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 40 Issue: 3 Pages: 365-374
  • DOI: 10.1248/bpb.b16-00880
  • NAID: 130005398514
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Superoxide generation from nNOS splice variants and its potential involvement in redox signal regulation (2017)
  • Author(s): Ihara H, Kitamura A, Kasamatsu S, Ida T, Kakihana Y, Tsutsuki H, Sawa T, Watanabe Y, Akaike T.
  • Journal Title: Biochem J Volume: 474 Issue: 7 Pages: 1149-1162
  • DOI: 10.1042/bcj20160999
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] レドックスシグナルの活性イオウ分子制御 (2017)
  • Author(s): 笠松真吾、守田匡伸、赤池孝章.
  • Journal Title: ファルマシア Volume: 53 Pages: 210-214
  • NAID: 130005398269
  • Peer Reviewed
• [Journal Article] Redox signaling regulated by an electrophilic cyclic nucleotide and reactive cysteine persulfides (2016)
  • Author(s): Fujii S, Sawa T, Nishida M, Ihara H, Ida T, Motohashi H, Akaike T.
  • Journal Title: Arch Biochem Biophys Volume: 595 Pages: 140-146
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Proposal of Helicobacter canicola sp. nov., previously identified as Helicobacter cinaedi, isolated from canines (2016)
  • Author(s): Kawamura Y, Tomida J, Miyoshi-Akiyama T, Okamoto T, Narita M, Hashimoto K, Cnockaert M, Vandamme P, Morita Y, Sawa T, Akaike T.
  • Journal Title: Syst Appl Microbiol Volume: 39 Issue: 5 Pages: 307-312
  • DOI: 10.1016/j.syapm.2016.06.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The chemicall biology of protein hydropersulfides: Studies of a possible protective function of biological hydropersulfide generateon. (2016)
  • Author(s): Millikin R, Bianco CL, White C, Saund SS, Henriquez S, Sosa V, Akaike T, Kumagai Y, Soeda S, Toscano JP, Lin J, Fukuto JM.
  • Journal Title: Free Radical Biology and Medicine Volume: 97 Pages: 136-147
  • DOI: 10.1016/j.freeradbiomed.2016.05.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mice Deficient in Angiopoietin-like Protein 2 (Angptl2) Gene Show Increased Susceptibility to Bacterial Infection Due to Attenuated Macrophage Activity (2016)
  • Author(s): Yugami M, Odagiri H, Endo M, Tsutsuki H, Fujii S, Kadomatsu T, Masuda T, Miyata K, Terada K, Tanoue H, Ito H, Morinaga J, Horiguchi H, Sugizaki T, Akaike T, Gotoh T, Takai T, Sawa T, Mizuta H, Oike Y
  • Journal Title: Journal of Biological Chemistry Volume: 291 Issue: 36 Pages: 18843-18852
  • DOI: 10.1074/jbc.m116.720870
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Endogenous occurrence of protein S-guanylation in Echerichia coli: Target identification and genetic regulation. (2016)
  • Author(s): Tsutsuki H, Jung M, Zhang T, Ono K, Ida T, Kunieda K, Ihara H, Akaike T, Sawa T.
  • Journal Title: Biochem Biophys Res Commun Volume: 478 Pages: 7-11
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Modification of tau by 8-nitro-cGMP: effects of nitric oxide-linked chemical modification on tau aggregation (2016)
  • Author(s): Yoshitake J, Soeda Y, Ida T, Sumioka A, Yoshikawa M, Matsushita K, Akaike T, Takashima A.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 43 Pages: 22714-22720
  • DOI: 10.1074/jbc.m116.734350
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protein polysulfidation-dependent persulfide dioxygenase activity of ethylmalonic encephalopathy protein 1 (2016)
  • Author(s): Jung M, Kasamatsu S, Matsunaga T, Akashi S, Ono K, Nishimura A, Morita M, Abdul Hamid H, Fujii S, Kitamura H, Sawa T, Ida T, Motohashi H, Akaike T.
  • Journal Title: Biochem Biophys Res Commun Volume: 480 Issue: 2 Pages: 180-186
  • DOI: 10.1016/j.bbrc.2016.10.022
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Redox signaling regulated by cysteine persulfide and protein polysulfidation (2016)
  • Author(s): Kasamatsu S, Nishimura A, Morita M, Matsunaga T, Abdul Hamid H, Akaike T.
  • Journal Title: Molecules Volume: 21 Issue: 12 Pages: 1721-1730
  • DOI: 10.3390/molecules21121721
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Re-emerging reactive sulfur-containing compounds and their unique biological functions (2016)
  • Author(s): 井田智章、松永哲郎、藤井重元、澤 智裕、赤池孝章.
  • Journal Title: Folia Pharmacologica Japonica Volume: 147 Issue: 5 Pages: 278-284
  • DOI: 10.1254/fpj.147.278
  • NAID: 130005150958
  • ISSN: 0015-5691, 1347-8397
  • Peer Reviewed / Open Access
• [Journal Article] Reactive sulfur species-modified protein thiols: new methods for polysulfurated protein analysis (2016)
  • Author(s): 笠松真吾、藤井重元、赤池孝章.
  • Journal Title: Folia Pharmacologica Japonica Volume: 147 Issue: 5 Pages: 299-302
  • DOI: 10.1254/fpj.147.299
  • NAID: 130005150953
  • ISSN: 0015-5691, 1347-8397
  • Peer Reviewed / Open Access
• [Journal Article] 巻頭言 セレンディピティと抗菌薬の新しい分指標的 (2016)
  • Author(s): 赤池孝章
  • Journal Title: 化学療法の領域 Volume: 32 Pages: 1621-1621
• [Presentation] Sulfur respiration in mammals and its implication for tumor biology (2018)
  • Author(s): Takaaki Akaike, Tomoaki Ida, Md. Morshedul Alam, Akira Nishimura, Masanobu Morita, Tetsuro Matsunaga, Hozumi Motohashi.
  • Organizer: The Cold Spring Harbor Asia Conference on Cancer & Metabolism
  • Int'l Joint Research / Invited
• [Presentation] 大腸菌におけるシステインパースルフィド生成機構 (2017)
  • Author(s): 井田智章、居原 秀、守田匡伸、笠松真吾、松永哲郎、西村 明、藤井重元、澤 智裕、赤池孝章.
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟（仙台市青葉区）
  • Year and Date: 2017-03-19
• [Presentation] 細菌のイオウ呼吸はすべての生物種に保存されている：ほ乳類における新しいエネルギー代謝経路・イオウ呼吸の発見 (2017)
  • Author(s): 赤池孝章、井田智章、松永哲郎、守田匡伸、笠松真吾、西村 明、藤井重元、居原 秀、ジョン ミンギョン、赤司壮一郎、澤 智裕、本橋ほづみ.
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟（仙台市青葉区）
  • Year and Date: 2017-03-19
• [Presentation] マクロファージにおけるヘリコバクター・シネディの持続感染の解析 (2017)
  • Author(s): 藤井重元、松永哲郎、西村 明、井田智章、澤 智裕、河村好章、赤池孝章.
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟（仙台市青葉区）
  • Year and Date: 2017-03-19
• [Presentation] システインパースルフィド産生酵素（Cysteine Persulfide Synthase, CPERS）の発見 (2017)
  • Author(s): 赤池孝章
  • Organizer: 第17回日本NO学会学術集会
  • Invited
• [Presentation] イオウ呼吸とイオウストレス：ほ乳類における新しいエネルギー代謝とレドックス病態 (2017)
  • Author(s): 赤池孝章、西村 明、井田智章、松永哲郎、守田匡伸、本橋ほづみ.
  • Organizer: 2017年度生理学研究所研究会「オルガネラダイナミクスの新規制御機構とその病態生理」
  • Invited
• [Presentation] イオウ呼吸とイオウストレス：新しいエネルギー代謝とレドックス病態の提案 (2017)
  • Author(s): 赤池孝章、西村 明、井田智章、松永哲郎、守田匡伸、藤井重元、本橋ほづみ.
  • Organizer: 第70回日本酸化ストレス学会学術集会
• [Presentation] Cysteine polysulfidation governed by cysteinyl-tRNA synthetase (CARSs) (2017)
  • Author(s): Takaaki Akaike
  • Organizer: Se2017 - 200 Years of Selenium Research (The 11th International Symposium on Selenium in Biology and Medicine and The 5th International Conference on Selenium in the Environment and Human Health)
  • Int'l Joint Research / Invited
• [Presentation] Cysteinyl-tRNA synthetase (CARS) controls endogenous hydropersulfide production and mediates mitochondrial functions and bioenegetics (2017)
  • Author(s): Takaaki Akaike
  • Organizer: S-Bio 2017 Plant and Human Sulfur Biology Conference 2017
  • Int'l Joint Research / Invited
• [Presentation] Discovery of a novel persulfide synthase that mediates mitochondrial sulfur respiration (2017)
  • Author(s): Takaaki Akaike
  • Organizer: 2nd Karolinska-Tohoku Joint Symposium on Medical Sciences
  • Int'l Joint Research / Invited
• [Presentation] Cysteinyl-tRNA synthetase governs endogenous hydropersulfide production and mediates mitochondrial functions and bioenergetics (2017)
  • Author(s): Takaaki Akaike
  • Organizer: AARS2017: IUBMB Focused Meeting on Aminoacyl-tRNA Synthetase
  • Int'l Joint Research
• [Presentation] Moonlighting functions of cysteinyl-tRNA synthetase: Cysteine hydropersulfide production and regulation of mitochondrial biogenesis and bioenergetics (2017)
  • Author(s): Takaaki Akaike, Akira Nishimura, Tomoaki Ida, Tetsuro Matsunaga, Masanobu Morita, Hozumi Motohashi.
  • Organizer: SfRBM 2017 - 24th Annual Meeting of the Society for Redox Biology and Medicine
  • Int'l Joint Research
• [Presentation] Cysteinyl-tRNA synthetase (CARS) は活性パースルフィド産生とミトコンドリア機能をコントロールしている (2017)
  • Author(s): 赤池孝章
  • Organizer: 2017年度生命科学系学会合同年次大会
  • Invited
• [Presentation] Cysteine persulfide formation via cysteinyl-tRNA synthetase and its involvement in mitochondrial bioenergetics (2017)
  • Author(s): Takaaki Akaike, Tomoaki Ida, Masanobu Morita, Shigemoto Fujii, Akira Nishimura, Tetsuro Matsunaga, Minkyung Jung, Hozumi Motohashi.
  • Organizer: 8th Joint Meeting of Society for Free Radical Research Australasia and Japan with International Symposium on Coenzyme Q10
  • Int'l Joint Research
• [Presentation] 活性イオウ分子種：システインポリスルフィドの生体機能と代謝制御 (2017)
  • Author(s): 赤池孝章
  • Organizer: 第19回日本質量分析学会北海道談話会講演会
  • Place of Presentation: 旭川医科大学教育推進センター カンファレンスルーム（旭川市）
  • Invited
• [Presentation] Persulfide regulation of mitochondrial number and function (2017)
  • Author(s): Takaaki Akaike
  • Organizer: The 2017 NO Gordon Research Conference
  • Place of Presentation: Ventura Beach Marriott (Venture, CA, USA)
  • Int'l Joint Research / Invited
• [Presentation] 細菌から真核生物に至る種横断的新規システインパースルフィド合成酵素の発見 (2017)
  • Author(s): 井田智章、赤池孝章.
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟（仙台市青葉区）
  • Invited
• [Presentation] Nitrated nucleotide-mediated antibacterial host defense and its regulation by hydrogen sulfide produced by bacteria (2016)
  • Author(s): Shigemoto Fujii, Shahzada Khan, Tetsuro Matsunaga, Tomoaki Ida, Katsuhiko Ono, Tomohiro Sawa, Takaaki Akaike.
  • Organizer: The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Year and Date: 2016-05-20
  • Int'l Joint Research
• [Presentation] Translation-coupled protein polysulfuration, a unique biosynthesis pathway of cysteine persulfide (2016)
  • Author(s): Tomoaki Ida, Hideshi Ihara, Fanyan Wei, Kazuhito Tomizawa, Shingo Kasamatsu, Masanobu Morita, Tetsuro Matsunaga, Yoshito Kumagai, Tomohiro Sawa, Hozumi Motohashi, Shigemoto Fujii, Takaaki Akaike.
  • Organizer: The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Year and Date: 2016-05-20
  • Int'l Joint Research
• [Presentation] 気道炎症と呼気NO (2016)
  • Author(s): 赤池孝章
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 京都国際会館（京都市左京区）
  • Invited
• [Presentation] Host defense and oxidative stress signaling in microbial infections (2016)
  • Author(s): Takaaki Akaike
  • Organizer: The 13th Korea-Japan International Symposium on Microbiology
  • Place of Presentation: The-K Hotel Gyeongju (Gyeongbuk, Korea)
  • Int'l Joint Research / Invited
• [Presentation] Cysteine transfer RNA synthetases moonlighting as novel cysteine persulfide synthases (CPERSs) (2016)
  • Author(s): Takaaki Akaike
  • Organizer: The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Int'l Joint Research / Invited
• [Presentation] 生体防御応答のレドックス制御機構 (2016)
  • Author(s): 赤池孝章
  • Organizer: 第27回日本生体防御学会学術総会
  • Place of Presentation: 九州大学病院キャンパス内 コラボステーションI 視聴覚ホール（福岡市東区）
  • Invited
• [Presentation] 8-ニトロ-cGMPとオートファジーによる細菌感染防御機構と硫化水素による制御 (2016)
  • Author(s): 藤井重元、松永哲郎、井田智章、小野勝彦、澤 智裕、赤池孝章.
  • Organizer: 第27回日本生体防御学会学術総会
  • Place of Presentation: 九州大学病院キャンパス内 コラボステーションI 視聴覚ホール（福岡市東区）
• [Presentation] システインtRNA合成酵素によるシステインパースルフィド生成とミトコンドリア機能制御 (2016)
  • Author(s): 井田智章、魏 范研、富澤一仁、守田匡伸、居原 秀、松永哲郎、笠松真吾、澤 智裕、藤井重元、赤池孝章.
  • Organizer: 第27回日本生体防御学会学術総会
  • Place of Presentation: 九州大学病院キャンパス内 コラボステーションI 視聴覚ホール（福岡市東区）
• [Presentation] Helicobacter cinaedi infection and atherosclerosis (2016)
  • Author(s): Takaaki Akaike, Tetsuro Matsunaga, Yoshiaki Kawamura, Shigemoto Fujii.
  • Organizer: 第48回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Int'l Joint Research / Invited
• [Presentation] 新しいCysteine Persulfide Synthaseの発見とそのミトコンドリア機能制御 (2016)
  • Author(s): 赤池孝章
  • Organizer: 生理学研究所研究会「オルガネラネットワークの制御機構とその生理的意義」
  • Place of Presentation: 生理学研究所1F大会議室（岡崎市）
  • Invited
• [Presentation] アミノアシルt-RNA合成酵素によるシステインパースルフィド合成とタンパク質ポリサルファ化 (2016)
  • Author(s): 井田智章、魏 范研、居原 秀、守田匡伸、笠松真吾、松永哲郎、富澤一仁、澤 智裕、本橋ほづみ、赤池孝章.
  • Organizer: 生理学研究所研究会「オルガネラネットワークの制御機構とその生理的意義」
  • Place of Presentation: 生理学研究所1F大会議室（岡崎市）
• [Presentation] Cysteinyl-tRNA synthetase controls protein polysulfidation and mitochondrial functions (2016)
  • Author(s): Takaaki Akaike
  • Organizer: The 2016 Thiol-Based Redox Regulation & Signaling Gordon Research Conference
  • Place of Presentation: Stoweflake Conference Center (Stowe, VT, USA)
  • Int'l Joint Research / Invited
• [Presentation] 新しいCysteine Persulfide Synthaseの発見とそのユニークなレドックス制御メカニズム (2016)
  • Author(s): 赤池孝章
  • Organizer: 第12回レドックス・ライフイノベーションシンポジウム
  • Place of Presentation: 熊本大学医学部医学教育図書棟3階第一講義室（熊本市中央区）
  • Invited
• [Presentation] 活性システインパースルフィドによるレドックス制御の分子基盤 (2016)
  • Author(s): 赤池孝章
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（札幌市白石区）
  • Invited
• [Presentation] 活性イオウ分子種によるミトコンドリア機能制御 (2016)
  • Author(s): 赤池孝章、井田智章.
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Invited
• [Presentation] 生体内タンパク質ポリスルフィドの検出とその生成機構 (2016)
  • Author(s): 赤池孝章、笠松真吾.
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Invited
• [Presentation] 新しいシステインパースルフィド合成酵素の発見とパースルフィドによるミトコンドリア機能制御機構の解明 (2016)
  • Author(s): 井田智章、魏 范研、松永哲郎、西田基宏、澤 智裕、西村明幸、守田匡伸、笠松真吾、居原 秀、藤井重元、熊谷嘉人、本橋ほづみ、赤池孝章.
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
• [Presentation] ニトロ化環状ヌクレオチドによる細菌感染防御機構と硫化水素による制御 (2016)
  • Author(s): 藤井重元、松永哲郎、井田智章、小野勝彦、澤 智裕、赤池孝章.
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
• [Presentation] 細菌感染におけるレドックスシグナル制御機構 (2016)
  • Author(s): 赤池孝章
  • Organizer: 第28回微生物学シンポジウム
  • Place of Presentation: 愛知学院大学薬学部（名古屋市千種区）
  • Invited
• [Presentation] Cysteinyl-tRNA synthetase is a major source of reactive persulfide and controls mitochondrial biology (2016)
  • Author(s): Takaaki Akaike
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Int'l Joint Research / Invited
• [Presentation] 生体内ポリサルファー代謝とレドックス制御機能 (2016)
  • Author(s): 藤井重元、澤 智裕、井田智章、笠松真吾、松永哲郎、守田匡伸、赤池孝章.
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター会議棟（仙台市青葉区）
  • Invited
• [Presentation] タンパク質ポリサルファ化の分子メカニズムの解明 (2016)
  • Author(s): 井田智章、魏 范研、笠松真吾、守田匡伸、松永哲郎、居原 秀、富澤一仁、熊谷嘉人、澤 智裕、本橋ほづみ、赤池孝章.
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 東北大学川内北キャンパス（仙台市青葉区）
• [Presentation] 活性イオウ生成酵素の発見：ミトコンドリア形態形成とエネルギー代謝の新しいメカニズム (2016)
  • Author(s): 赤池孝章
  • Organizer: 第14回がんとハイポキシア研究会
  • Place of Presentation: 岐阜グランドホテル（岐阜市）
  • Invited
• [Book] Nitric Oxide: Biology and Pathobiology. 3rd ed. (Ignarro LJ, Freeman BA, eds.) (2017)
  • Author(s): Sawa T, Kumagai Y, Akaike T.
  • Total Pages: 403
  • Publisher: Academic Press
  • ISBN: 9780128042731
• [Remarks] 東北大学大学院医学系研究科 環境医学分野教室ホームページ
  • URL: http://www.toxicosci.med.tohoku.ac.jp
• [Remarks] 東北大学大学院医学系研究科環境保健医学分野ホームページ
  • URL: http://www.toxicosci.med.tohoku.ac.jp/