Development of a novel method for the identification of ncRNA-binding proteins
| Project/Area Number |
16K15212
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
KUBOTA YUJI 東京大学, 医科学研究所, 助教 (70614973)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 非コードRNA / 蛋白質 / 機能性RNA / 核酸 / 細胞 / 生体分子 / バイオテクノロジー / プロテオーム |
|---|
| Outline of Final Research Achievements |
Non-coding (nc) RNAs are functional RNA molecules that are transcribed from genomic DNA. Recent studies have demonstrated that various ncRNAs (e.g., miRNAs and lncRNAs) play important roles in the regulation of diverse cellular functions such as cell proliferation, and apoptosis through interacting with specific RNAs or proteins. Furthermore, accumulating evidence showed that abnormal expression of ncRNAs is involved in the etiology of human diseases including cancers. However, the functions of individual ncRNAs remain largely unknown. In this study, we aimed to establish a novel method to identify proteins that selectively bind to a specific ncRNA. For this purpose, we performed several pilot studies using a representative lncRNA, whose binding partners have already been identified. By constructing various expression vectors and optimizing experimental conditions, we succeeded in establishing the novel method. This method will greatly advance our understanding of ncRNAs.
|
Report
(3 results)
Research Products
(78 results)
-
-
-
-
-
-
-
-
-
[Journal Article] Genomic loss of DUSP4 contributes to the progression of intraepithelial neoplasm of pancreas to invasive carcinoma.2016
Author(s)
Hijiya N, Tsukamoto Y, Nakada C, Tung NL, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.
-
Journal Title
Cancer Res.
Volume: 76
Issue: 9
Pages: 2612-2625
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] DUSP4の発現低下は膵上皮内癌から浸潤癌への進展に関与する2016
Author(s)
Naoki Hijiya, Yoshiyuki Tsukamoto, Chisato Nakata, Tomoki Kai, Keiko Matsuura, Masafumi Inomata, Kuniaki Shirao, Hiromu Mori, Masao Seto, Masahiro Aoki, Mutsuhiro Takekawa, Masatsugu Moriyama
Organizer
第75回日本癌学会学術総会
Place of Presentation
パシフィコ横浜（横浜）
Year and Date
2016-10-06
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
