Investigation of the transcriptional coregulator involved in crosstalk between histone acetylation and methylation
| Project/Area Number |
16K15216
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 転写コレギュレーター / ヒストン修飾 / 細胞周期 / 生体分子 |
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| Outline of Final Research Achievements |
Nardilysin (NRDC) is a metalloendopeptidase of the M16 family and activates ectodomain cleavage of membrane proteins on the cell surface. On the other hand, nuclear NRDC specifically binds to H3K4me2 and controls transcriptional regulation. Analysis of genetically-modified mice revealed that NRDC play important roles in cancer, inflammation and energy metabolism. In the present study, we combined high-throughput chromatin immunoprecipitation-sequencing and RNA-sequencing analyses to achieve the first genome-wide identification of NRDC target genes in immortalized mouse embryo fibroblasts. We found that global H3K9ac and H3K4me2 levels at NRDC-binding promoters were controled and that a set of genes integral to cell cycle maintenance was directly activated by NRDC. Collectively, these results suggest that the NRDC-mediated epigenetic regulation of cell cycle-associated genes is a fundamental component of the biological functions of NRDC.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Nardilysin controls intestinal tumorigenesis through HDAC1/p53-dependent transcriptional regulation.2018
Author(s)
Kanda K, Sakamoto J, Matsumoto Y, Ikuta K, Goto N, Morita Y, Ohno M, Nishi K, Eto K, Kimura Y, Nakanishi Y, Ikegami K, Yoshikawa T, Fukuda A, Kawada K, Sakai Y, Ito A, Yoshida M, Kimura T, Chiba T, Nishi E, Seno H.
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Journal Title
JCI insight
Volume: 3
Issue: 8
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nardilysin promotes hepatocellular carcinoma through activation of signal transducer and activator of transcription 32017
Author(s)
Kasai Y, Toriguchi K, *Hatano E, Nishi K, Ohno M, Yoh T, Fukuyaman K, Nishio T, Okuno M, Iwaisako K, Seo S, Taura K, Kurokawa M, Kunichika M, Uemoto S, *Nishi E
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Journal Title
Cancer Sci
Volume: 108
Pages: 910-17
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction2017
Author(s)
Wang H,Zhang X-M,Tomiyoshi G,Nakamura R,Shinmen N,Kuroda H,Kimura R,Mine S,Kamitsukasa I,Wada T,Aotsuka A,Yoshida Y,Kobayashi E,Matsutani T,Iwadate Y,Sugimoto K,Mori M,Uzawa A,Muto M,Kuwabara S,Takemoto M,Kobayashi K,Kawamura H,Ishibashi R,Yokote K,Ohno M,
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Journal Title
Oncotarget
Volume: 9
Pages: 5600-5613
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Loss of Nardilysin, a mitochondrial co-chaperone for α-Ketoglutarate Dehydrogenase, promotes mTORC1 activation and neurodegeneration.2017
Author(s)
Yoon W.H., Sandoval H.,Nagarkar-Jaiswal S.,Jaiswal M.,Yamamoto S., Haelterman N.A., Putluri N., Putluri V., Sreekumar A., Tos T., Aksoy A., Donti T., Graham B.H., Ohno M., Nishi E., Hunter J., Muzny D.M.,Carmichael J.,Shen J.,Arboleda V.A.,Nelson S.F.,Wangler M.F.,Karaca E.,Lupski J.R.,Bellen H.
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Journal Title
Neuron
Volume: 93
Pages: 115-131
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Nardilysin is required for maintaining pancreatic β-Cell Function.2016
Author(s)
Nishi K, Sato Y, Ohno M, Hiraoka Y, Saijo S, Sakamoto J, Chen P-M, Morita Y, Matsuda S, Iwasaki K, Sugizaki K, Harada N, Mukumoto Y, Kiyonari H, Furuyama K, Kawaguchi Y, Uemoto S, Kita T, Inagaki N, Kimura T and Nishi E
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Journal Title
Diabetes.
Volume: 65
Pages: 3015-27
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] ナルディライジンは神経栄養因子受容体(p75NTR)のシェディングを介して心臓交感神経の分布を制御する2017
Author(s)
大野美紀子, 西 清人, 平岡 義範, 新妻 晋一郎, 西城 さやか, 坂本 二郎, 陳 博俊, 森田 雄介, 松田 真太郎, 椋本 淑子, 清成 寛, 松浦 博, 北 徹, 木村 剛, 西 英一郎.
Organizer
生命科学系学会合同年次大会(第40回日本分子生物学会年会)
Related Report
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[Presentation] Nardilysin is a Promising Biomarker for the Early Diagnosis of Acute Coronary Syndrome.2016
Author(s)
Ohno M, Chen P-M, Hiwasa T, Nishi K, Saijo S, Sakamoto J, Morita Y, Watanabe S, Kuwabara Y, Ono K, Imai M, Inoue K, Murai T, Kita T, Kimura T, and Nishi E.
Organizer
American Heart Association Scientific Session
Place of Presentation
New Orleans, LA
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] Nardilysin, a novel biomarker for hepatocellular carcinoma, enhances diethylnitrosamine-induced mice carcinogenesis and cancer cell proliferation via activation of STAT3 signaling pathway2016
Author(s)
Kasai Y, Hatano E, Nishi E, Toriguchi K, Yoh T, Nishio T, Okuno M, Seo S, Taura K, Yasuchika K, Okajima H, Kaido T, Uemoto S
Organizer
International Liver Cancer Association 2016
Place of Presentation
Vancouber
Year and Date
2016-09-09
Related Report
Int'l Joint Research
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