Exploration of the possibility of involvement of ectopic meiosis in lethal phenotype of Max knockout embryos
| Project/Area Number |
16K15223
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
OKUDA AKIHIKO 埼玉医科大学, 医学部, 教授 (60201993)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 生殖細胞 / 減数分裂 / PRC1 / MAX / MGA / ES細胞 / コンディショナルノックアウトマウス / 異所性減数分裂 / Maxノックアウトマウス / Max / 異所性 |
|---|
| Outline of Final Research Achievements |
Germ cells need to disrupt transcription repressing function of PRC1.6 to onset meiosis. In this study, I investigated the molecular bases of impairment of PRC1.6 function. I also tried to examine whether forced disruption of the function of PRC1.6 is accompanied with ectopic onset of meiosis. With these studies, I identified germ cell specific Mga variant which is supposed to disrupt the function of PRC1.6. Furthermore, I observed up-regulation in expression levels of meiosis-related genes in male primordial germ cells by homozygous knockout of Max gene.
|
Report
(3 results)
Research Products
(15 results)
-
-
-
[Journal Article] Identification of the coiled-coil domain as an essential Mbd3 element for preserving lineage commitment potential of embryonic stem cells2018
Author(s)
Masataka Hirasaki, Atsushi Ueda, Masamitsu N. Asaka, Kousuke Uranishi, Ayumu Suzuki, Masakazu Kohda, Yosuke Mizuno, Yasushi Okazaki, Masazumi Nishimoto, Jafar Sharif, Haruhiko Koseki, Akihiko Okuda
-
Journal Title
Stem Cells
Volume: 印刷中
Issue: 9
Pages: 1355-1367
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
[Book] 実験医学2018
Author(s)
奥田晶彦
Total Pages
6
Publisher
羊土社
ISBN
9784758125055
Related Report
-
