| Project/Area Number |
16K15225
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
Murakoshi Hideji 生理学研究所, 脳機能計測・支援センター, 准教授 (90608142)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | シグナル伝達 / LOV2 / 蛍光タンパク質 / ペプチド |
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| Outline of Final Research Achievements |
In this project, we aimed to develop optogenetic tools to selectively activate a specific pathway of biochemical signaling in synapses. These tools enable us to identify the essential pathways and signaling molecules required for the induction of long-term potentiation. We especially focused on developing a new imaging probe that can be used in combination of light sensitive proteins. As a result of our research, we successfully developed a new probe optimized for 2-photon Fluorescence Lifetime Imaging (2pFLIM) based Forster resonance energy transfer (FRET).
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