Role of ER stress-induced ncRNA in renal cancer pathogenesis
| Project/Area Number |
16K15237
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OYADOMARI Seiichi 徳島大学, 先端酵素学研究所(プロテオ), 教授 (90502534)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 小胞体ストレス / 腎癌 / ncRNA / 分子病態学 |
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| Outline of Final Research Achievements |
In the final year, we planned to comprehensively analyze the molecular mechanism of the tumor promotion effect by the identified ncRNA by the next generation sequencer. As a preliminary experiment, it was found that it takes time to establish isolation of RNA that interacts with ncRNA using UV cross-linking. On the other hand, we have successively established the knockout mice line of the identified ncRNA, therefore, analysis of the phenotype of the knockout mice was given priority.
As a result, the homozygous knockout mice found to die after birth from respiratory failure because of their small thorax due to dyschondrogenesis. On the other hands, the heterozygous knockout mice showed no apparently different difference from the wild type, but were extremely sensitive and had high mortality in ER stress condition. Taken together, we can clarify the physiological significance of adaptation fo ER stress of the novel ncRNA.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] The Role of Heparin Cofactor Ⅱ in the Regulation of Insulin Sensitivity and Maintenance of Glucose Homeostasis in Humans and Mice2017
Author(s)
Kurahashi K, Inoue S, Yoshida S, Ikeda Y, Morimoto K, Uemoto R, Ishikawa K, Kondo T, Yuasa T, Endo I, Miyake M, Oyadomari S, Matsumoto T, Abe M, Sakaue H, Aihara KI.
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Journal Title
Journal of Atherosclerosis and Thrombosis
Volume: 24
Issue: 12
Pages: 1215-1230
DOI
NAID
ISSN
1340-3478, 1880-3873
Related Report
Peer Reviewed / Open Access
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[Journal Article] ES Stress Protein CHOP Mediates Insulin Resistanse by Modulating Adipose Tissue Macrophage Polarity.2017
Author(s)
Suzuki T, Gao J, Ishigaki Y, Kondo K, Sawada S, Izumi T, Uno K, Kaneko K, Tsukita S, Takahashi K, Asao A, Ishii N, Imai J, Yamada T, Oyadomarai S, Katagiri H
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Journal Title
Cell Rep
Volume: 18
Issue: 8
Pages: 2045-2057
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Correction: Long-Term Low Carbohydrate Diet Leads to Deleterious Metabolic Manifestations in Diabetic Mice.2016
Author(s)
Handa K, Inukai K, Onuma H, Kudo A, Nakagawa F, Tsugawa K, Kitahara A, Moriya R, Takahashi K, Sumitani Y, Hosaka T, Kawakami H, Oyadomari S, Ishida H.
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Journal Title
PLoS One
Volume: 11
Issue: 5
Pages: e0155751-e0155751
DOI
Related Report
Peer Reviewed
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