Is the iPS marker "TRA-1-60" an indicator of cancer intractability against therapeutics?

• Project/Area Number: 16K15245
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Niigata University
• Principal Investigator: Kondo Eisaku 新潟大学, 医歯学系, 教授 (30252951)
• Co-Investigator(Kenkyū-buntansha): 奥田 修二郎 新潟大学, 医歯学系, 准教授 (00512310) ; 阪口 政清 岡山大学, 医歯薬学総合研究科, 准教授 (70379840)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膵がん / TRA-1-60 / PODXL1 / 転移 / 浸潤 / 病理学

Outline of Final Research Achievements

1. PODXL1-konckout clones were generated from human three different PDAC lines since the TRA-1-60 is the glycosylated form of PODXL1. Consequently, drastic inhibition of liver metastasis in vivo was observed in all PODXL1-KO clone-xenografted mice generated from MiaPaCa-2, AsPC1, Panc-1.
2. PODXL1 revealed to form complex with multiple cytokine receptors and its binding contribute to activate those receptors.
3. PODXL1 was highly expressed at the invasive front and metastatic foci of PDAC cells in patients' tumor tissues.

Research Products

• [Journal Article] Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation. (2018)
  • Author(s): Muhammad K, Rudolf R, Pham DAT, Klein-Hessling S, Takata K, Matsushita N, Ellenrieder V, Kondo E, Serfling E.
  • Journal Title: Front. Immunol. Volume: 9
  • DOI: 10.3389/fimmu.2018.00032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of tumor-lineage homing peptide for their tailored application to diverse malignancies (2017)
  • Author(s): 近藤英作、斎藤憲
  • Journal Title: 生化学 Volume: 89 Issue: 1 Pages: 44-50
  • DOI: 10.14952/SEIKAGAKU.2017.890044
  • ISSN: 0037-1017
  • Year and Date: 2017-02-25
• [Journal Article] 生体機能性ペプチドを応用した次世代ナノメディシンの展開 (2017)
  • Author(s): 近藤英作
  • Journal Title: 生化学 Volume: 89 Issue: 1 Pages: 7-7
  • DOI: 10.14952/SEIKAGAKU.2017.890007
  • ISSN: 0037-1017
  • Year and Date: 2017-02-25
• [Journal Article] ｂ-1,3-galactosyl-O-glycosyl-glycoprotein ｂ-1,6-N-acetylglucosaminyltransferase 3 Increases MCAM Stability, Which Enhances S100A8/A9-Mediated Cancer Motility. (2017)
  • Author(s): Sumardika I Wayan、Youyi Chen、Kondo Eisaku、Inoue Yusuke、Ruma I Made Winarsa、Murata Hitoshi、Kinoshita Rie、Yamamoto Ken-Ichi、Tomida Shuta、Shien Kazuhiko、Satoh Hiroki、Yamauchi Akira、Futami Junichiro、Putranto Endy Widya、Hibino Toshihiko、Toyooka Shinichi、Nishibori Masahiro、Sakaguchi Masakiyo
  • Journal Title: Oncology Reserch Volume: in press Issue: 3 Pages: 431-444
  • DOI: 10.3727/096504017x15031557924123
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of a novel component leading to anti-tumor activity besides the major ingredient cordycepin in Cordyceps militaris extract. (2017)
  • Author(s): Wada T, Sumardika IW, Saito S, Ruma IMW, Kondo E, Shibukawa M, Sakaguchi M
  • Journal Title: J Chromatogr B Analyt Technol Biomed Life Sci. Volume: - Pages: 209-219
  • DOI: 10.1016/j.jchromb.2017.07.022
  • Peer Reviewed
• [Journal Article] Differences in Urinary Renal Failure Biomarkers in Cancer Patients Initially Treated with Cisplatin (2017)
  • Author(s): Maeda A, Ando H, Ura T. Muro K, Aoki M, Saito K, Kondo E, Takahashi S, Ito Y, Mizuno Y, Fujimura A
  • Journal Title: Anticancer Research Volume: 37 Issue: 9 Pages: 5235-5339
  • DOI: 10.21873/anticanres.11947
  • Peer Reviewed
• [Journal Article] Association between and polymorphisms and adverse drug reactions to regorafenib: A preliminary study (2017)
  • Author(s): Maeda A, Ando H, Ura T, Komori A, Hasegawa A, Taniguchi H, Kadowaki S, Muro K, Tajika M, Kobara M, Matsuzaki M, Hashimoto N, Maeda M, Kojima Y, Aoki M, Kondo E, Mizutani A, Fujimura A.
  • Journal Title: Int. J. Clin. Pharmacol. Ther Volume: epub ahead of print Issue: 05 Pages: 409-415
  • DOI: 10.5414/cp202788
  • Peer Reviewed
• [Journal Article] Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1. (2016)
  • Author(s): Matsushita, N., Suzuki, M., Ikebe, E., Nagashima, S., Inatome, R., Asano, K., Tanaka, M., Matsushita, M., Kondo, E., Iha, H., and Yanagi, S.
  • Journal Title: Sci. Rep Volume: 6 Issue: 1 Pages: 31266-31266
  • DOI: 10.1038/srep31266
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Active Secretion of Dimerized S100A11 Induced by the Peroxisome in Mesothelioma Cells. (2016)
  • Author(s): Saho S, Satoh H, Kondo E, Inoue Y, Yamauchi A, Murata H, Kinoshita R, Yamamoto KI, Futami J, Putranto EW, Ruma IM, Sumardika IW, Youyi C, Suzawa K, Yamamoto H, Soh J, Tomida S, Sakaguchi Y, Saito K, Iioka H, Huh NH, Toyooka S, Sakaguchi M.
  • Journal Title: Cancer Microenviron. Volume: 9 Issue: 2-3 Pages: 93-105
  • DOI: 10.1007/s12307-016-0185-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Peptide-based tumor inhibitor encoding mitochondorial p14ARF is highly efficacious to diverse tumors (2016)
  • Author(s): K. Saito, H. Iioka, C. Kojima, M. Ogawa, E. Kondo
  • Journal Title: Cancer Science Volume: 107 Issue: 9 Pages: 1290-1301
  • DOI: 10.1111/cas.12991
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MCAM, as a novel receptor for S100A8/A9, mediates progression of malignant melanoma through prominent activation of NF-κB and ROS formation upon ligand binding. (2016)
  • Author(s): Ruma IM, Putranto EW, Kondo E, Murata H, Watanabe M, Huang P, Kinoshita R, Futami J, Inoue Y, Yamauchi A, Sumardika IW, Youyi C, Yamamoto K, Nasu Y, Nishibori M, Hibino T, Sakaguchi M.
  • Journal Title: Clin Exp Metastasis Volume: 33 Issue: 6 Pages: 609-627
  • DOI: 10.1007/s10585-016-9801-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Peptide-based tumor tecnology using tumorhoming CPPs. (2017)
  • Author(s): Eisaku Kondo
  • Organizer: The 4th RIKEN/Karolonska Insitutet/SciLifeLab Joint Symposium
  • Int'l Joint Research / Invited
• [Presentation] 濾胞性リンパ腫難治性を規定する特殊細胞集団の発見 (2017)
  • Author(s): 近藤英作,高田尚良,齋藤憲
  • Organizer: 第76回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] S100A8/A9とその受容体との結合遮断を目指した転移抑制タンパク質製剤の開発 (2017)
  • Author(s): 木下理恵,山内明,枝園和彦,富田秀太,豊岡伸一,近藤英作,坂口政清
  • Organizer: 第76回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] Crumbs3は焦点接着構成因子を制御することで大腸癌の転移を促進する (2017)
  • Author(s): 飯岡英和,齋藤憲,坂口政清,森井英一,近藤英作
  • Organizer: 第76回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] R-loop 蓄積と関連するTREX2複合体の機能不全は乳がん細胞において化学療法感受性を増大させる (2017)
  • Author(s): 桑原一彦,近藤英作
  • Organizer: 第76回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] 口腔扁平上皮癌におけるPLOD2の発現と細胞移動能との関連 (2017)
  • Author(s): 植木雄志,齋藤憲,近藤英作
  • Organizer: 第76回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] PLOD2は口腔がん細胞の移動能を制御する (2017)
  • Author(s): 齋藤憲,植木雄志,近藤英作
  • Organizer: 第76回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] 生体内腫瘍におけるR-loop 形成亢進の特徴と意義 (2017)
  • Author(s): 栗田彩花,桑原一彦,近藤英作
  • Organizer: 第14回日本病理学会カンファレンス
• [Presentation] 口腔がん細胞相互作用におけるPLOD2の役割 (2017)
  • Author(s): 斎藤憲,植木雄志,近藤英作
  • Organizer: 第21回日本がん分子標的治療学会
• [Presentation] 乳癌細胞においてTREX2複合体構成分子は薬剤感受性誘導の標的となる (2017)
  • Author(s): 桑原一彦,近藤英作
  • Organizer: 第21回日本がん分子標的治療学会
• [Presentation] R-loop発現の病理学的意義 (2017)
  • Author(s): 田中萌恵,桑原一彦,近藤英作
  • Organizer: 第106回日本病理学会総会
• [Presentation] 多様な系統の癌における薬物代謝酵素CYP3A5の発現とその意義 (2017)
  • Author(s): 田坂杏美,田中萌恵,桑原一彦,植木雄志,齋藤憲,近藤英作
  • Organizer: 第106回日本病理学会総会
• [Presentation] 濾胞性リンパ腫難治性を規定する特殊細胞集団の発見 (2017)
  • Author(s): 近藤英作,高田尚良,齋藤憲
  • Organizer: 第106回日本病理学会総会
• [Presentation] Crumbs3は大腸癌細胞の接着性制御を介し腫瘍形成を促進する (2017)
  • Author(s): 飯岡英和,齋藤憲,森井栄一,近藤英作
  • Organizer: 第106回日本病理学会総会
• [Presentation] 病理学のアドバンテージを活かした腫瘍医学研究へのアプローチ (2017)
  • Author(s): 近藤英作
  • Organizer: 第106回日本病理学会総会
  • Invited
• [Presentation] 抗がん治療増強の標的としてのTREX2複合体 (2017)
  • Author(s): 桑原一彦,近藤英作
  • Organizer: 第106回日本病理学会総会
• [Presentation] 肺がん選択的透過ペプチドの開発 (2017)
  • Author(s): 斎藤憲,飯岡英和,近藤英作
  • Organizer: 第106回日本病理学会総会
• [Presentation] Forced reduction of DSS1, a member of TREX2 complex, highly sensitizes chemotherapy to breast cancer cells in a BRCA2-independent manner. (2017)
  • Author(s): Kazuhiko Kuwahara, Eisaku Kondo
  • Organizer: AACR Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] Cellular interaction between pancreatic cancer cells and MSC as tumor microenvironment. (2017)
  • Author(s): Eisaku Kondo, Ken Saito
  • Organizer: AACR Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] 浸潤性膵管癌におけるがん-間質相互反応の増殖・浸潤における役割 (2016)
  • Author(s): 近藤英作,齋藤憲,飯岡英和,坂口政清
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 難治性肺がん治療に向けた抗腫瘍ペプチドの開発 (2016)
  • Author(s): 齋藤憲,飯岡英和,近藤英作
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 口腔扁平上皮癌におけるlysyl hydroxylaseの機能 (2016)
  • Author(s): 植木雄志、斎藤憲、近藤英作
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] ナノメディシンへの応用を目指したがんホーミングペプチドの開発 (2016)
  • Author(s): 近藤英作
  • Organizer: 第105回日本病理学総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] 腫瘍系統選択的に吸収される特殊機能ペプチドの開発とその臨床学的応用 (2016)
  • Author(s): 斎藤憲、近藤英作
  • Organizer: 第105回日本病理学総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] 細胞極性制御因子Crumbs3は大腸癌の腫瘍形成を亢進させる (2016)
  • Author(s): 飯岡 英和、斎藤憲、近藤英作
  • Organizer: 第105回日本病理学総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
• [Book] ペプチド医薬品の スクリーニング・安定化・製剤化技術 (2017)
  • Author(s): 近藤英作
  • Total Pages: 557
  • Publisher: 技術情報協会
• [Book] 医療・診断を支えるペプチド科学 (2017)
  • Author(s): 齋藤憲、近藤英作
  • Total Pages: 315
  • Publisher: シーエムシー出版
  • ISBN: 9784781312675
• [Remarks] 新潟大学大学院医歯学総合研究科分子細胞病理学
  • URL: http://www.med.niigata-u.ac.jp/pa2/index.html