Development of combinational therapy of non-thermal plasma and carbon nanotubes
| Project/Area Number |
16K15257
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
KIKKAWA Fumitaka
KAJIYAMA Hiroaki
HORI Masaru
TANAKA Hiromasa
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 低温プラズマ / カーボンナノチューブ / フェロトーシス / ナノマテリアル / 中皮腫 / フェリチン / 医療応用 / 鉄 / キレート / オートファジー |
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| Outline of Final Research Achievements |
We obtained fundamental data for the use of combinational therapy of non-thermal plasma (NTP) and carbon nanotubes. We identified hemoglobin, histones and transferrin as specifically adsorbed proteins on carbon nanotube surface with mass spectrometry. Biological effects of NTP exposure were recognized as oxidative stress with biochemical, FACS and immunostaining studies, which was promoted with preincubation with ferric ammonium citrate and repressed by preincubation with redox-inactive iron chelator, desferal. NTP exposure to mesothelioma cells caused an increase in intracellular catalytic Fe(II), lipid peroxidation with increased ferritin/decreased transferrin receptor, which was accompanied by promoted endocytosis and autophagy (ferritinophagy). These data suggest that NTP can cause ferroptosis in mesothelioma cells. As in vitro experiments we also observed that NTP exposure can directly destroy the ferritin core protein and reduce Fe(III) to Fe(II).
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Non-Thermal Plasma Induces a Stress Response in Mesothelioma Cells Resulting in Increased Endocytosis, Lysosome Biogenesis and Autophagy.2017
Author(s)
Shi. L., Ito, F., Wang, Y., Okazaki, Y., Tanaka, H., Mizuno, M., Hori. M., Hirayama, T., Nagasawa, H., Richardson, Des R, Toyokuni, S.
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Journal Title
Free Radic. Biol. Med.
Volume: 108
Pages: 904-917
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease.2017
Author(s)
Stockwell BR, Friedmann Angeli JP, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascon S, Hatzios SK, Kagan VE, Noel K, Jiang X, Linkermann A, Murphy ME, Overholtzer M, Oyagi A, Pagnussat GC, Park J, Ran Q, Rosenfeld CS, Salnikow K, Tang D, Torti FM, Torti SV, Toyokuni S, Woerpel KA, Zhang DD.
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Journal Title
Cell.
Volume: 171
Issue: 2
Pages: 273-285
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis.2016
Author(s)
Wang Y, Okazaki Y, Shi L, Kohda H, Tanaka M, Taki K, Nishioka T, Hirayama T, Nagasawa H, Yamashita Y, Toyokuni S.
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Journal Title
Cancer Sci
Volume: 107(3)
Issue: 3
Pages: 250-257
DOI
Related Report
Peer Reviewed / Open Access
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