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Analysis of Non-coding RNA Y1 during iPS reprogramming

Research Project

Project/Area Number 16K15260
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Gojo Satoshi  京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsReprogramming / Non-coding RNA / iPS細胞 / リプログラミング / non-coding RNA
Outline of Final Research Achievements

Non coding RNA RNY1 (Y1) is a transiently expressed factor during early iPS reprogramming, and induction efficiency to iPS cells is decreased by its knockdown. We analyzed the molecular machinery related to RNY1 and RO60, which form a complex, during cellular reprogramming. DDX6 protein, which involves in RNA metabolism was identified, and the knockout cell for DDX6 also showed a drastic decrease in induction efficiency. We found that the RNY1/RO60/DDX6 axis attributes to mesenchymal epithelial transition, which is essential for initial phase in iPS reprogramming. and proposed a putative molecular model for parental mRNA decay in the nexus of a set of miRNAs.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2018

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] RNA helicase DDX6を中心としたRNA代謝とiPS細胞リプログラミング過程における役割2018

    • Author(s)
      上大介、木谷友哉、五條理志
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Role in RNA metabolism of processing on RNA helicase DDX6 during iPS reprogramming2018

    • Author(s)
      Daisuke Kami, Tomoya Kitani, Satoshi Gojo
    • Organizer
      International Society for Stem Cell Research 15th Annual Meeting.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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