| Project/Area Number |
16K15260
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Gojo Satoshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Reprogramming / Non-coding RNA / iPS細胞 / リプログラミング / non-coding RNA |
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| Outline of Final Research Achievements |
Non coding RNA RNY1 (Y1) is a transiently expressed factor during early iPS reprogramming, and induction efficiency to iPS cells is decreased by its knockdown. We analyzed the molecular machinery related to RNY1 and RO60, which form a complex, during cellular reprogramming. DDX6 protein, which involves in RNA metabolism was identified, and the knockout cell for DDX6 also showed a drastic decrease in induction efficiency. We found that the RNY1/RO60/DDX6 axis attributes to mesenchymal epithelial transition, which is essential for initial phase in iPS reprogramming. and proposed a putative molecular model for parental mRNA decay in the nexus of a set of miRNAs.
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