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Preparation of Bordetella pertussis that infects experimental animals by introduction of large DNA fragments from B. bronchiseptica

Research Project

Project/Area Number 16K15276
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionOsaka University

Principal Investigator

Horiguchi Yasuhiko  大阪大学, 微生物病研究所, 教授 (00183939)

Co-Investigator(Renkei-kenkyūsha) SHINZAWA Naoaki  東京医科歯科大学, 大学院医学科, 助教 (10583015)
NAKAMURA Keiji  九州大学, 大学院医学研究院, 研究員 (60706216)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords百日咳菌 / 気管支敗血症菌 / 百日咳
Outline of Final Research Achievements

Bordetella pertussis (Bp) is a strictly human-adapted pathogen. The Aim of this study is to generate Bp mutants by introducing large DNA fragments into Bp genomes from B. bronchiseptica (Bb), which infects broad range of mammalians. If Bp mutants that infect experimental animals were successfully obtained, it should be a powerful tool to dissect the pathogenesis of Bp through animal infection models. To accomplish this purpose, we developed a novel method designated BBI system to enable us to introduce ~50 kb of DNA fragments into genomes of recipient bacteria. Our results revealed that the DNA fragment introduced by BBI system could be functional in the recipient bacteria; however, after screening of more than 1,500 clones of Bp, into which large DNA fragments were introduced from B. bronchiseptica, we could not obtain mutant strains of Bp that readily infects rats. We are now conducting further work to obtain Bp mutants which infects small experimental animals.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Ectopic expression of O antigen in Bordetella pertussis by a novel genomic intergration system2018

    • Author(s)
      K. Ishigaki, N. Shinzawa, S. Nishikawa, K. Suzuki, A. Fukui-Miyazaki, Y. Horiguchi
    • Journal Title

      mSphere

      Volume: 3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Ectopic expression of O-antigen in Bordetella pertussis by a novel genomic integration system2017

    • Author(s)
      石垣佳祐、新澤直明、西川明芳、鈴木孝一朗、堀口安彦
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台市、宮城県
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] O抗原合成に関わる長鎖ゲノム領域の相補によるO抗原発現百日咳菌の作製2016

    • Author(s)
      石垣佳祐、新澤直明、西川明芳、堀口安彦
    • Organizer
      第159回 日本獣医学会学術集会
    • Place of Presentation
      藤沢市、神奈川県
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Development of the novel genomic integration system to study the host tropism of Bordetella spp.2016

    • Author(s)
      K.Ishigaki, S.Nishikawa, N.Shinzawa, Y.Horiguchi
    • Organizer
      The 13th Korea-Japan International Symposium on Microbiology
    • Place of Presentation
      慶州市、韓国
    • Year and Date
      2016-05-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of the novel genomic integration system to study the host tropism of Bordetella spp.2016

    • Author(s)
      K.Ishigaki, S.Nishikawa, N.Shinzawa, Y.Horiguchi
    • Organizer
      The 11th International Bordetella Symposium
    • Place of Presentation
      ブエノスアイレス、アルゼンチン
    • Year and Date
      2016-04-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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