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mechanism of diversification of shiga toxin-encoding phage, from the ecological aspect

Research Project

Project/Area Number 16K15278
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionKyushu University

Principal Investigator

Yoshitoshi Ogura  九州大学, 医学研究院, 准教授 (40363585)

Co-Investigator(Renkei-kenkyūsha) NAKAOKA shinji  東京大学, 生産技術研究所, 特任助教 (30512040)
Research Collaborator ARIMIZU Yoko  九州大学, 医学研究院, 大学院生
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords志賀毒素 / ファージ / 大腸菌 / 共進化 / 原生生物 / 腸管出血性大腸菌 / 進化 / ゲノム
Outline of Final Research Achievements

We constructed three Stx2 phage lysogenes of Escherichia coli K-12 and the lysogenes were infected to Dictyosteluim discoideum AX2. But growth of D. discoideum was not affected by the infection of the lysogens at all. Even original O157 strains were not toxic to D. discoideum. Furthermore, inconsistent with several previous studies, we found that infection of O157 strains was not toxic to Acanthamobae castellanii. It is thought that another model protist is required for this analysis. We also showed that the recent and repeated acquisition of the stx2 are occurred in multiple lineages of O26. This suggest the presence of selective pressure to accumulate Stx2 in E. coli O26 in environment. Protist that colonize in bovine intestine is thought to be one of the candidates of the selective pressure.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results)

  • [Int'l Joint Research] Liege university(Belgium)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Population structure of Escherichia coli O26:H11 with recent and repeated stx2 acquisition in multiple lineages2017

    • Author(s)
      Ogura Yoshitoshi、Gotoh Yasuhiro、Itoh Takehiko、et al.
    • Journal Title

      Microbial Genomics

      Volume: 3 Issue: 11

    • DOI

      10.1099/mgen.0.000141

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Genome analysis of bovine commensal E. coli; novel insights into the evolutional pathway of EHEC2018

    • Author(s)
      Yoshitoshi Ogura
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 大規模比較ゲノム解析による腸管出血性大腸菌出現プロセスの解明2017

    • Author(s)
      中島遥子、桐野有美、宇野浩一、佐藤寿夫、佐藤光彦、西田留梨子、吉野修司、大岡唯祐、後藤恭宏、谷沢靖洋、中村 保一、井口純、石原朋子、大西真、林哲也、小椋義俊
    • Organizer
      第20回腸管出血性大腸菌感染症研究会
    • Place of Presentation
      富山県民共生センターサンフォルテ2階ホール
    • Year and Date
      2017-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] 国内外で分離された521株の腸管出血性大腸菌O26の全ゲノム系統解析と病原遺伝子レパートリー解析2017

    • Author(s)
      小椋義俊、黒木真理子、吉野修司、木全恵子、磯部順子、勢戸和子、前田詠里子、江藤良樹、楠本正博、秋庭正人、石嶋希、李謙一、伊豫田淳、大西真、大岡唯祐、 後藤恭宏、林哲也
    • Organizer
      第90回 日本細菌学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 大規模比較ゲノム解析による腸管出血性大腸菌の起源と出現プロセスの解明2017

    • Author(s)
      中島遥子、桐野有美、宇野浩一、佐藤寿夫、佐藤光彦、西田留梨子、吉野修司、大岡唯祐、後藤恭宏、谷沢靖洋、中村 保一、井口純、石原朋子、大西真、林哲也、小椋義俊
    • Organizer
      第11回日本ゲノム微生物学会年会
    • Place of Presentation
      慶應義塾大学 湘南藤沢キャンパス
    • Year and Date
      2017-03-02
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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