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Comprehensive identification of host factors which contribute to replication of herpes virus using genome editing

Research Project

Project/Area Number 16K15280
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

Kawaguchi Yasushi  東京大学, 医科学研究所, 教授 (60292984)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsウイルス / HSV / ゲノム編集
Outline of Final Research Achievements

In this study, (i) we created platform for efficient screening of host factors responsible for HSV replication, by constructing reporter virus in which reporter gene products were incorporated into HSV virions. (ii) Using the reporter virus, several host factors which contribute to HSV replication were identified. Furthermore, we clarified that these host factors were important for intracellular transport of HSV. (iii) We demonstrated that host ESCRT-III machinery is used for HSV budding through the inner nuclear membrane, by analyzing host factors that contribute to viral particle formation.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、HSVの増殖に必要な宿主因子を効率的にスクリーニングするためのプラットフォームを構築し、HSV増殖に必要な宿主因子を複数同定しただけでなく、長年にわたり未解明であったHSVカプシドの核外輸送の分子機構を解明した点で、学術的に高い意義を有していると考えられる。また、本研究成果によって構築したスクリーニング法は、HSV感染症に対する宿主細胞因子を標的とした創薬への応用に繋がることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity.2018

    • Author(s)
      Arii J, Watanabe M, Maeda F, Tokai-Nishizumi N, Chihara T, Miura M, Maruzuru Y, Koyanagi N, Kato A, Kawaguchi Y.
    • Journal Title

      Nat Commun.

      Volume: 9 Issue: 1 Pages: 3379-3379

    • DOI

      10.1038/s41467-018-05889-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Herpes Simplex Virus 1 VP22 Inhibits AIM2-Dependent Inflammasome Activation to Enable Efficient Viral Replication.2018

    • Author(s)
      Maruzuru Y, Ichinohe T, Sato R, Miyake K, Okano T, Suzuki T, Koshiba T, Koyanagi N, Tsuda S, Watanabe M, Arii J, Kato A, Kawaguchi Y.
    • Journal Title

      Cell Host Microbe.

      Volume: 23 Issue: 2 Pages: 254-265

    • DOI

      10.1016/j.chom.2017.12.014

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis.2017

    • Author(s)
      Koyanagi N, Imai T, Shindo K, Sato A, Fujii W, Ichinohe T, Takemura N, Kakuta S, Uematsu S, Kiyono H, Maruzuru Y, Arii J, Kato A, Kawaguchi Y.
    • Journal Title

      J Clin Invest.

      Volume: 127 Issue: 10 Pages: 3784-3795

    • DOI

      10.1172/jci92931

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete fusion of a transposon and herpesvirus created the Teratorn mobile element in medaka fish2017

    • Author(s)
      Inoue Yusuke、Saga Tomonori、Aikawa Takumi、Kumagai Masahiko、Shimada Atsuko、Kawaguchi Yasushi、Naruse Kiyoshi、Morishita Shinichi、Koga Akihiko、Takeda Hiroyuki
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 551-551

    • DOI

      10.1038/s41467-017-00527-2

    • NAID

      120006822516

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Herpes Simplex Virus 1 Small Capsomere-Interacting Protein VP26 Regulates Nucleocapsid Maturation.2017

    • Author(s)
      Kobayashi R, Kato A, Sagara H, Watanabe M, Maruzuru Y, Koyanagi N, Arii J, Kawaguchi Y.
    • Journal Title

      J Virol.

      Volume: 91 Issue: 18

    • DOI

      10.1128/jvi.01068-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Herpes Simplex Virus 1 UL34 Protein Regulates the Global Architecture of the Endoplasmic Reticulum in Infected Cells.2017

    • Author(s)
      F. Maeda, J. Arii, Y. Hirohata, Y. Maruzuru, N. Koyanagi, A. Kato and Y. Kawaguchi.
    • Journal Title

      J. Virol.

      Volume: 印刷中 Issue: 12

    • DOI

      10.1128/jvi.00271-17

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 2016 International meeting of the Global Virus Network2017

    • Author(s)
      Akkina Ramesh、Ellerbrok Heinz、Hall William、Hasegawa Hideki、Kawaguchi Yasushi、Kleanthous Harold、McSweegan Edward、Mercer Natalia、Romanowski Victor、Sawa Hirofumi、Vahlne Anders
    • Journal Title

      Antiviral Research

      Volume: 142 Pages: 21-29

    • DOI

      10.1016/j.antiviral.2017.03.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A single amino acid substitution in HSV-1 UL31 at an inter-hexamer contact site abrogates primary envelopment and viral growth2018

    • Author(s)
      Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
    • Organizer
      第66回日本ウイルス学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Residues in α-helix 9 of HSV-1 UL31 is critical for interaction with UL25 and incorporation of capsid into vesicles in the perinuclear space2018

    • Author(s)
      Kosuke Takeshima, Jun Arii, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
    • Organizer
      第66回日本ウイルス学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A single amino acid substitution in herpes simplex virus 1 UL31 at an inter-hexamer contact site abrogates primary envelopment and viral growth2018

    • Author(s)
      Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
    • Organizer
      43rd Annual International Herpesvirus Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of amino acid residues in the α-helix 9 of HSV-1 UL31 in its interaction with capsid proteins and nuclear egress2018

    • Author(s)
      Kosuke Takeshima, Jun Arii, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
    • Organizer
      43rd Annual International Herpesvirus Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel Mechanisms for Immune Evasion by Herpes Simplex virus2018

    • Author(s)
      Yasushi Kawaguchi
    • Organizer
      International Joint Forum on Infectious Disease Research 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inhibition of AIM2 inflammasome activation by an HSV-1 virion component enables efficient viral replication in vivo2017

    • Author(s)
      Yuhei Maruzuru, Takeshi Ichinohe, Naoto Koyanagi, Jun Arii, Akihisa Kato and Yasushi Kawaguchi
    • Organizer
      第31回ヘルペスウイルス研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] Evasion of AIM2-Dependent Inflamasome Activation by Herpes Simplex Virus 1 Virion Protein Enables Efficient Viral Replication In Vivo2017

    • Author(s)
      Yuhei Maruzuru, Takeshi Ichinohe, Naoto Koyanagi, Zhuoming Liu, Tomoko Ando, Jun Arii, Akihisa Kato, Yasushi Kawaguchi
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 単純ヘルペスウイルスの宿主免疫回避機構2017

    • Author(s)
      川口 寧
    • Organizer
      日本生化学会北陸支部第35回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Inhibition of AIM2 inflammasome activation by an HSV-1 virion component enables efficient viral replication in vivo2017

    • Author(s)
      Yuhei Maruzuru, Takeshi Ichinohe, Naoto Koyanagi, Jun Arii, Akihisa Kato and Yasushi Kawaguchi
    • Organizer
      42nd Annual International Herpesvirus Workshop
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Novel Mechanisms for Immune Evasion by herpes simplex virus2017

    • Author(s)
      Yasushi Kawaguchi
    • Organizer
      24th East Asia Joint Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of a novel interaction between the endosomal sorting protein SNX3 and HSV-1 membrane proteins2016

    • Author(s)
      Akihisa Kato, Hayato Harima, Jun Arii, Yasushi Kawaguchi
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Endosomal sorting protein SNX3とHSV膜蛋白質の相互作用に関する解析2016

    • Author(s)
      加藤哲久, 播磨勇人, 有井潤, 川口寧
    • Organizer
      第30回ヘルペスウイルス研究会
    • Place of Presentation
      セミナーハウス クロス・ウェーブ府中(東京都府中市)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Research-status Report
  • [Remarks] 東京大学医科学研究所 感染・免疫部門 ウイルス病態制御分野

    • URL

      http://www.ims.u-tokyo.ac.jp/Kawaguchi-lab/KawaguchiLabTop.html

    • Related Report
      2018 Annual Research Report 2017 Research-status Report 2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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