Single cell analysis of T lymphocytes in the intestinal immune system

• Project/Area Number: 16K15294
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Keio University
• Principal Investigator: HASE Koji 慶應義塾大学, 薬学部(芝共立), 教授 (20359714)
• Co-Investigator(Kenkyū-buntansha): 城口 克之 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (00454059)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 大腸炎 / シングルセル解析 / TCRレパトア解析 / 腸管免疫系 / 腸管免疫 / 免疫学

Outline of Final Research Achievements

Conventional methods such as flow cytometry and quantitative PCR have limitation to analyze individual lymphocyte cells. Here we established single cell analysis method by taking advantage of DNA barcoding, and analyzed TCR repertoire in the gut of experimental colitis model.

Research Products

• [Journal Article] Intestinal Epithelial Cell-specific Deletion of α-Mannosidase II Ameliorates Experimental Colitis (2018)
  • Author(s): Suzuki K., Yamada T, Yamazaki K, Hirota M, Ishihara N, Sakamoto M, Takahashi D, Iijima H, Hase K.
  • Journal Title: Cell Structure and Function Volume: 43 Issue: 1 Pages: 25-39
  • DOI: 10.1247/csf.17022
  • NAID: 130006513071
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access
• [Journal Article] Macrophage extracellular chromatin release induced by Mac-1 mediated platelet-macrophage interactions triggers acute kidney injury in rhabdomyolysis. (2018)
  • Author(s): Okubo K, Kurosawa M, Kamiya M, Urano Y, Suzuki A, Yamamoto K, Hase K, Homma K, Sasaki J, Miyauchi H, Hoshino T, Hayashi M, Mayadas TN, Hirahashi J.
  • Journal Title: Nat. Med. Volume: 24 Issue: 2 Pages: 232-238
  • DOI: 10.1038/nm.4462
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] IL-22BP dictates characteristics of Peyer's patch follicle-associated epithelium for antigen uptake. (2017)
  • Author(s): Jinnohara T, Kanaya T, Hase K, Sakakibara S, Kato T, Tachibana N, Sasaki T, Hashimoto Y, Sato T, Watarai H, Kunisawa J, Shibata N, Williams I, Kiyono H, and Ohno H.
  • Journal Title: J. Exp. Med. Volume: in press
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Zinc transporter SLC39A7/ZIP7 promotes intestinal epithelial self-renewal by resolving ER stress. (2016)
  • Author(s): Ohashi, W., Kimura, S., Iwanaga, T., Furusawa, Y., Irie, T., Izumi, H., Watanabe, T., Hijikata, A., Hara, T., Ohara, O., Koseki, H., Sato, T., Robine, S., Mori, H., Hattori, Y., Watarai, H., Mishima, K., Ohno, H., Hase, K., Fukada, T.
  • Journal Title: PLoS Genetics Volume: 12 Issue: 10 Pages: e1006349-e1006349
  • DOI: 10.1371/journal.pgen.1006349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation. (2016)
  • Author(s): Kimura S, Yamashita M, Yamakami-Kimura M, Sato Y, Yamagata A, Kobashigawa Y, Inagaki F, Amada T, Hase K, Iwanaga T, Ohno H, Fukai S.
  • Journal Title: Sci. Rep. Volume: 15 Pages: 33548-33548
  • NAID: 120005855947
  • Peer Reviewed / Open Access
• [Presentation] Microbiota-derived metabolites shape mucosal barrier & immunity (2017)
  • Author(s): Koji Hase
  • Organizer: Korean Society of Nephrology (KSN) 2017
  • Int'l Joint Research / Invited
• [Presentation] Microbiota-derived metabolites regulate mucosa immunity and barrier, (2016)
  • Author(s): Hase K.
  • Organizer: The 45th Annual Meeting of The Japanese Society for Immunology.
  • Place of Presentation: Okinawa Convention Center (Okinawa, Ginowan)
  • Year and Date: 2016-12-06
  • Int'l Joint Research / Invited