Development of a novel labeling method for affibody by F-18 labeled amino acid and a cell-free protein synthesis system
| Project/Area Number |
16K15314
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉川 雄朗 東北大学, 医学系研究科, 准教授 (70506633)
原田 龍一 東北大学, 医学系研究科, 助教 (60735455)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 分子イメージング / 放射性医薬品 / タンパク質 / PET |
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| Outline of Final Research Achievements |
In this study, we tried to develop a novel labeling method for affibody molecular that possess high binding affinity to targets and fast pharmacokinetics by F-18 labeled amino acid and a cell-free protein synthesis system. 18F-O-2-fluoroethyl tyrosine (FET) was successfully incorporated into proteins by using commercially available cell-free protein synthesis reagents with an engineered aminoacyl transferase/ tRNACUAopt pair and template DNA of the desired proteins inserted with an amber codon. 18F-labeled affibody for human epidermal growth factor receptor type 2 (HER2) demonstrated that high binding to SKOV-3 expressing HER2 with high level. In vivo PET imaging in SKOV-3 xenograft-bearing mice showed that high tracer accumulation in the SKOV-3 xenografts. In conclusion, this method might be useful for preparation of proteins with F-18 and molecular imaging in preclinical development.
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Report
(3 results)
Research Products
(1 results)
