The photodynamic detection of MRD of multiple myeloma by flowcytometory

Research Project

Project/Area Number	16K15324
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Laboratory medicine
Research Institution	Tohoku University
Principal Investigator	HARIGAE HIDEO 東北大学, 医学系研究科, 教授 (50302146)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	微小残存病変 / フローサイトメトリ― / アミノレブリン酸 / フローサイトメトリー / 内科 / 癌
Outline of Final Research Achievements	Heme is generated in mitochondria by insertion of iron to protoporphyrin IX (PPIX). As the activity of mitochondria is decreased in cancer cells, PPIX is accumulated by the administration of ALA. The aim of this study is to establish the novel method to detect minimal residual disease (MRD) of multiple myeloma by applying this characteristic of cancer cell biology to flowcytometory. First, the condition to detect the fluorescence of PPIX was established in KMS18 cell, a myeloma cell line. It was confirmed that the established condition can effectively differentiates KMS18 cells from normal blood cells. Next, transporters which incorporate ALA into cells, were identified by the competition assay. Then the movement of ALA after incorporated into cells was clarified using Caco cells. As a result, a part of ALA was used for the generation of PPIX, and unused ALA was exported from cells. These findings ｍay contribute to the development of a novel detection system of MRD.