| Project/Area Number |
16K15328
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Ehime University |
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Principal Investigator |
Yamamoto Shin 愛媛大学, 医学系研究科, 寄附講座助教 (70646992)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 非アルコール性脂肪肝炎 / 第二次高調波発生 / 自家蛍光 / NASH / 2光子励起顕微鏡 / SHGシグナル / 2光子励起顕微鏡(TPEM) / 非アルコール性脂肪肝炎症(NASH) / 第二次高調波発生(SHG) / 自家蛍光(AF) / ビタミンA / in vivo イメージング / 第2高調波発生 |
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| Outline of Final Research Achievements |
The non-alcoholic steatohepatitis (NASH) has been increasing. The definitive diagnosis of NASH requires histopathological examination of liver biopsy samples. Optical imaging techniques make it possible to visualize structure, morphology, and molecular distribution in vivo at the cellular level. Fibrosis can be observed directly without staining due to a nonlinear optical phenomenon called second harmonic generation (SHG). Here we demonstrate SHG and auto-fluorescence (AF) imaging targeting ex vivo liver tissues in NASH model mice. Using three-dimensional (3D) SHG and AF imaging, image processing by maximum intensity projection (MIP), and statistical analyses, we successfully characterized the fibrotic and morphological changes in hepatic capsule and hepatic parenchyma. The direct and quantitative SHG and AF imaging method presented in this study could be applied to diagnosis of NASH.
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