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Development of novel carcinogenicity prediction system using Nucling gene deficient mouse

Research Project

Project/Area Number 16K15333
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionTokushima Bunri University

Principal Investigator

SAKAI TAKASHI  徳島文理大学, その他の研究科, 教授 (80284321)

Research Collaborator LIN Wenling  徳島文理大学, 大学院
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords発がん / 炎症性疾患 / 肝がん / インスリンシグナル / 発がん性試験 / 炎症性ストレス
Outline of Final Research Achievements

We confirmed the usefulness of Nucling-KO mouse from various viewpoints as a material for new carcinogenicity prediction system. The KO mice fed normal chow diet grow in the same manner as the wild-type under the SPF environment, but after 12 months of age the incidence of inflammatory diseases such as hepatitis and liver cancer rose significantly. The effects of high fat diet or high sugar diet administration (body weight gain, impaired glucose tolerance, insulin resistance etc.) appeared at a high rate in KO mice. Examination of the gene expression pattern revealed abnormalities in the expression level of insulin and its regulators in KO mice. With the administration of hepatotoxic reagent DEN, onset of fatty liver, hepatitis, and liver cancer could be confirmed in KO mice significantly earlier than wild type. From the above, it is expected that it will be a useful prediction system for at least searching for substances that are stressful to the liver.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2016

All Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Presentation] 新規NF-κB 制御分子ヌクリングはインスリンの発現制御に関与している2016

    • Author(s)
      林 文琳、坂井 隆志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] A novel apoptosis associated protein, Nucling Controls Mammary Gland Involution by Regulating NF-B and STAT3, Implication for the pathogenesis of breast cancer2016

    • Author(s)
      1)Dang HV, Sakai T, Pham TA, Tran DH, Yorita K, Shishido Y, Fukui K
    • Organizer
      Inaugural Symposium Biochemistry: Towards Academic Excellenc
    • Place of Presentation
      スリランカ・ボレールズガマワ
    • Year and Date
      2016-06-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2019-03-29  

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