| Outline of Final Research Achievements |
In this study, we examined two new detection methods: digital nucleic acid quantification method with reverse transcription and digital nucleic acid quantification method of identifying and quantifying nucleic acid amplified by melting curve analysis. The isothermal amplification method can amplify a nucleic acid only by maintaining it at around 70 ° C, but there is a problem that it can not be determined unless there is a 10-fold concentration difference, but digitization of the isothermal amplification kit with reverse transcription It has been found that it becomes possible to detect even a concentration difference of about 2 times. In addition, in order to detect genomic mutations used for quality control of cells utilized for regenerative medicine of retina, newly designed primers for isothermal amplification, digital nucleic acid amplification, and biotyped and mutated nucleic acid by melting curve analysis. It turned out that it can be identified and quantified.
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