A new method for body fluid identification by analyzing microRNA and probabilistic interpretation
| Project/Area Number |
16K15401
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Tamaki Keiji 京都大学, 医学研究科, 教授 (90217175)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | DNA多型学 / マイクロRNA / 体液識別 / DNA多型医学 / microRNA / RNA / 法医物体検査 |
|---|
| Outline of Final Research Achievements |
In forensic casework, body fluid identification (BFID) plays a prominent role for the reconstruction of criminal activity. We aimed to establish a novel BFID method based on microRNA expression, and collected venous blood, saliva, semen, vaginal secretion, and skin. We revealed that venous blood and semen could be identified by using of miR-144-3p and miR-451a-5p for blood, and miR-888-5p and miR-891a-5p for semen, and that saliva and vaginal secretion could be discriminated by quadratic discriminant analysis by using the expression of miR-203a and miR-1260b. Furthermore, for the investigation of the effect of individual characteristics on our BFID method in sexual cases, we examined these microRNA in infertile semen and vaginal secretion at the follicular and luteal phases. These microRNA can be also detected in infertile semen and vaginal secretion at the both phases. Therefore, our BFID method will be applicable in actual forensic casework.
|
Report
(3 results)
Research Products
(8 results)
