| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
It is important to clarify the pathological changes of organs / tissues after death in investigating the cause of death. However, there are limitations in image photographing and histological examination in the past, and it is difficult to prove the presence of brain dysfunction such as mental disorders, acute encephalopathy, sleep apnea syndrome, arrhythmia, etc. after death. Therefore, in this research, we focused on the brain, visualized the brain after death and examined its functional evaluation. As to transparency, it was completely transparent in the brain, lungs and pancreas, whereas in the heart, liver, spleen some color remained. When nerve fiber staining and Hoechst nuclear staining were performed on the transparent brain, clear images were obtained in Hoechst, and a tissue-clearing protocol which was the most important task could be established.
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