Development of Medaka NASH Model and Hepatocarcinogenesis

• Project/Area Number: 16K15424
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Niigata University
• Principal Investigator: Terai Shuji 新潟大学, 医歯学系, 教授 (00332809)
• Co-Investigator(Kenkyū-buntansha): 山際 訓 新潟大学, 医歯学系, 准教授 (10419327) ; 上村 顕也 新潟大学, 医歯学系, 助教 (00579146) ; 川合 弘一 新潟大学, 医歯学総合病院, 講師 (80419291) ; 高村 昌昭 新潟大学, 医歯学総合病院, 講師 (20422602) ; 土屋 淳紀 新潟大学, 医歯学総合病院, 助教 (70464005)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: メダカモデル / NASH / SGLT2阻害剤 / 発癌モデル

Outline of Final Research Achievements

The development of Medaka NASH model was examined. The drR medaka was utilized to develop the model. High fsat diet was continuously administered to the model and the liver tissue, blood biochemistry were casrefully examined to deteremine whether the NASH model mimicking the condition in the human.
The results showed that the successful development of the model. Following this success, the prevention of the progression of NASH in Medaka liver was assessed using SGLT2 inhibitor. Surprisingly, the SGLT2 inhibitor successfully inihibited the progression of NASH in these model.

Research Products

• [Journal Article] Effect of histidine on sorafenib-induced vascular damage: Analysis using novel medaka fish model. (2018)
  • Author(s): Shinagawa-Kobayashi Y, Kamimura K, Goto R, Ogawa K, Inoue R, Yokoo T, Sakai N, Nagoya T, Sakamaki A, Abe S, Sugitani S, Yanagi M, Fujisawa K, Nozawa Y, Koyama N, Nishina H, Furutani- Seiki M, Sakaida I, Terai S.
  • Journal Title: Biochem Biophys Res Commun. Volume: 496 Issue: 2 Pages: 556-561
  • DOI: 10.1016/j.bbrc.2018.01.057
  • Peer Reviewed
• [Presentation] メダカを用いたSGLT2阻害薬によるNASH進展抑制効果の検証 (2017)
  • Author(s): 後藤 諒(新潟大学 大学院消化器内科学), 上村 顕也, 小林 陽子, 井上 良介, 小川 光平, 坂牧 僚, 阿部 聡司, 寺井 崇二
  • Organizer: 第59回日本消化器病学会大会