Establishment of human pluripotent stem cell-derived bile ductal disease analysis system using microfluidic device

• Project/Area Number: 16K15434
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokai University
• Principal Investigator: KAMIYA Akihide 東海大学, 医学部, 准教授 (30321904)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ヒト多能性幹細胞 / 肝分化 / 胆管細胞 / 肝幹細胞 / 再生医療 / 再生医学 / マイクロ・ナノデバイス

Outline of Final Research Achievements

The liver is the central organ of maintenance of the metabolism of the living body, and a method of reconstituting the mini-liver from human pluripotent stem cells in vitro has been developed. On the other hand, it has a disadvantage that it does not have a structure of the functional bile duct network which is important for the function of the liver.
In this study, we are developing a culture system that can be applied to the analysis and treatment of human bile duct disease, by constructing a system that induces bile duct like structures from human iPS cell-derived hepatic progenitor cell. We also analyzed about the efficient culture system of mini-liver tissue using fluid devices.

Research Products

• [Journal Article] Characteristics of hepatic progenitor cells during liver development and regeneration. (2017)
  • Author(s): Kamiya A, Chikada H.
  • Journal Title: Stem Cells in Clinical Applications “Liver, Lung and Heart Regeneration” (edited by Phuc Van Pham) Volume: 1 Pages: 3-13
  • DOI: 10.1007/978-3-319-46693-4_1
  • ISBN: 9783319466927, 9783319466934
• [Journal Article] Identification of a novel alpha-fetoprotein- expressing cell population induced by Jagged1/Notch2 signal in murine fibrotic liver. (2017)
  • Author(s): Yasuhiro Nakano, Sachie Nakao, Hideaki Sumiyoshi, Kenichiro Mikami, Yuri Tanno, Minako Sueoka, Daigo Kasahara, Hiroshi Kimura, Tadashi Moro, Akihide Kamiya, Katsuto Hozumi, Yutaka Inagaki
  • Journal Title: Hepatol Commun Volume: 印刷中 Issue: 3 Pages: 1-15
  • DOI: 10.1002/hep4.1026
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Foetal hepatic progenitor cells assume a cholangiocytic cell phenotype during two-dimensional pre-culture. (2016)
  • Author(s): Kazuya Anzai, Hiromi Chikada, Kota Tsuruya, Kinuyo Ida, Tatehiro Kagawa, Yutaka Inagaki, Tesuya Mine, Akihide Kamiya
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 28283-28283
  • DOI: 10.1038/srep28283
  • Peer Reviewed / Open Access
• [Presentation] 肝前駆細胞の成熟化を誘導する転写調節機構 (2017)
  • Author(s): 紙谷聡英、安斎和也、鶴谷康太、近田裕美
  • Organizer: 第17回日本再生医療学会