Elucidating the molecular pathology of inclusion body myositis using novel culture system and next generation sequencer
Project/Area Number |
16K15474
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Neurology
|
Research Institution | Tohoku University |
Principal Investigator |
Aoki Masashi 東北大学, 医学系研究科, 教授 (70302148)
|
Co-Investigator(Kenkyū-buntansha) |
割田 仁 東北大学, 大学病院, 助教 (30400245)
鈴木 直輝 東北大学, 大学病院, 助教 (70451599)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 封入体筋炎 / 筋疾患 / 次世代シークエンサー / iPS細胞 / 共培養モデル |
Outline of Final Research Achievements |
As for sIBM, patient samples were collected and clinical information was also collected at the same time. The significance of autoantibody pathology in patient serum was analyzed by human serum and mouse experiment in collaboration with Kumamoto University. CD56 positive skeletal myoblasts from some skeletal muscles were collected and established from 8 sIBM cases. RNA sequencing was performed on 4 cases of sIBM. Including comparison with 9 hereditary inclusive body myopathies, disease-related reported mutations and rare variants were extracted. We are also analyzing mice model based on proteasome dysfunction hypothesis.
|
Report
(3 results)
Research Products
(5 results)
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[Journal Article] Pathomechanisms of anti-cytosolic 5'-nucleotidase 1A autoantibodies in sporadic inclusion body myositis.2017
Author(s)
Tawara N, Yamashita S, Zhang X, Korogi M, Zhang Z, Doki T, Matsuo Y, Nakane S, Maeda Y, Sugie K, Suzuki N, Aoki M, Ando Y.
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Journal Title
Ann Neurol.
Volume: 81
Issue: 4
Pages: 512-525
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Sporadic Inclusion Body Myositis Manifesting as Isolated Muscle Weakness of the Finger Flexors Three Years after Disease Onset2016
Author(s)
Suwa Y, Suzuki N, Soga T, Harada R, Shibui A, Kuroda H, Izumi R, Tateyama M, Nakashima I, Sonoo M, Aoki M.
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Journal Title
Internal Medicine
Volume: 55
Issue: 23
Pages: 3521-3524
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Multicenter questionnaire survey for sporadic inclusion body myositis in Japan.2016
Author(s)
Suzuki N, Mori-Yoshimura M, Yamashita S, Nakano S, Murata KY, Inamori Y, Matsui N, Kimura E, Kusaka H, Kondo T, Higuchi I, Kaji R, Tateyama M, Izumi R, Ono H, Kato M, Warita H, Takahashi T, Nishino I, Aoki M.
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Journal Title
Orphanet J Rare Dis.
Volume: 11
Issue: 1
Pages: 146-146
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Multicenter questionnaire survey for sporadic inclusion body myositis in Japan.2017
Author(s)
Naoki Suzuki, Madoka Mori-Yoshimura, Satoshi Yamashita, Satoshi Nakano, Ken-ya Murata, Yukie Inamori,Naoko Matsui, En Kimura, Hirofumi Kusaka, Tomoyoshi Kondo, Itsuro Higuchi, Ryuji Kaji, Maki Tateyama,Rumiko Izumi, Hiroya Ono, Masaaki Kato, Hitoshi Warita, Toshiaki Takahashi, Ichizo Nishino and Masashi Aoki
Organizer
World Congress of Neurology 2017
Related Report
Int'l Joint Research
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[Presentation] Proteasomal proteolysis is indispensable for the maintenance of skeletal muscle and muscle stem cells2016
Author(s)
Naoki Suzuki, Yasuo Kitajima, Yoshitaka Tashiro, Hiroya Ono, Risa Ando, Shion Osana, Aki Nunomiya, Ryoichi Nagatomi, Ryosuke Takahashi, Masashi Aoki
Organizer
21th World Muscle Society
Place of Presentation
Granada, Spain
Related Report
Int'l Joint Research