| Project/Area Number |
16K15479
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ゲノム編集 / タウオパチー / CRISPR / Cas9 / MAPT / スプライシング / CRISPR-Cas9 / 脳神経疾患 / 痴呆 / 遺伝子 / ゲノム |
|---|
| Outline of Final Research Achievements |
MAPT gene polymorphism is known in diseases that allow accumulation of tau, but it was difficult to analyze the significance of the polymorphism. By using the CORRECT method applying CRISPR-Cas 9 method, we introduce disease-related MAPT polymorphisms and investigate the expression level of tau gene and its effect on splicing. The introduction rate of genome editing improves 1.0% by standard method up to 4.5%. However, the efficiency was still low, and it was revealed that there is a possibility that it may be markedly different depending on genes. When splicing of MAPT was examined using the edited cells, it was confirmed that the ratio of 4R/3Rtau was increased. This cell line can be applied to drug screening to improve the ratio.
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