| Project/Area Number |
16K15481
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Hiroshima University |
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Principal Investigator |
Kawakami Hideshi 広島大学, 原爆放射線医科学研究所, 教授 (70253060)
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| Co-Investigator(Kenkyū-buntansha) |
森野 豊之 広島大学, 原爆放射線医科学研究所, 准教授 (10397953)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | TDP-43 / 筋萎縮性側索硬化症 / 脳神経疾患 / 神経科学 / 遺伝学 |
|---|
| Outline of Final Research Achievements |
Aggregates of TAR DNA-binding protein of 43 kDa(TDP-43)are characteristic in motorneuron in amyotrophic lateral sclerosis. To identify genes influencing TDP-43 aggregate formation, we infected lentivirus from the library with CRISPR and the recognition sequence and destroy genes. We expressed TDP-43 with eGFP and isolated cells with enhanced signals by cell sorter. We sequenced DNA extracted from cells by next-generation sequencer, and identified 30 genes as TDP-43 aggregate formation related.
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