| Project/Area Number |
16K15484
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Juntendo University |
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Principal Investigator |
Imai Yuzuru 順天堂大学, 医学(系)研究科(研究院), 先任准教授 (30321730)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIBA Kahori 順天堂大学, 医学研究科, 准教授 (30468582)
INOSHITA Tsuyoshi 順天堂大学, 医学研究科, 助教 (20601206)
OHBA Yusuke 北海道大学, 医学部, 教授 (30333503)
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| Research Collaborator |
ARANO Taku 順天堂大学, 医学研究科, 研究員 (80750091)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ミトコンドリア / ドーパミン神経 / パーキンソン病 / 神経変性疾患 / 創薬 / Parkin / PINK1 / iPS / ショウジョウバエ / スクリーニング / シグナル伝達 / 脳神経疾患 / 酵素 |
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| Outline of Final Research Achievements |
The genes responsible for autosomal recessive early-onset Parkinson’s disease, Parkin and PINK1 encode a ubiquitin ligase and a mitochondrial protein kinase, respectively. A series of studies have revealed that Parkin in collaboration with PINK1 has a role for mitochondrial quality control, impairment of which leads to dopaminergic neurodegeneration. In this study, we aimed at searching drugs to improve mitochondrial functions through moderate activation of PINK1-Parkin signaling.
We developed a cell-based reporter assay to monitor PINK1-Parkin signaling and screened approximately 310-thousands of chemicals. Hit compounds obtained in the screening were further tested for mitochondrial membrane potential and cell viability and three candidates were identified as seed compounds.
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