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Rejuvenation of stem cells, tissues and individuals

Research Project

Project/Area Number 16K15489
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Metabolomics
Research InstitutionKyoto University

Principal Investigator

Takashima Yasuhiro  京都大学, iPS細胞研究所, 特定拠点講師 (70469930)

Research Collaborator Ueda Mai  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords多能性幹細胞 / ナイーブ型 / プライム型 / ナイーブ型多能性幹細胞 / DNAメチレーション / 老化 / 幹細胞 / 糖尿病 / 再生医学
Outline of Final Research Achievements

We succeeded in rejuvenating human primed ES/iPS cells to more undifferentiated human naive iPS cells using reprogramming techniques. In this naive process, iPS cells are demethylated. We try to nalyze and clarify the process that is reset from primed typed to naive type ES/iPS cells. Detailed time course and gene expression of resetting from primed type to naive type ES/iPS cells were analyzed. As a result, we developed a method to induce naive ES/iPS cells more efficiently and stably.
In the future, we will compare the findings obtained here with the progression of methylation in somatic stem cells and somatic cells, and apply a method of demethylation we find, which was observed at naive ES/iPS cells and resetting process, to suppress unnecessary methylation.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Surface markers guide the journey towards naive pluripotency2017

    • Author(s)
      Karagiannis P, Takashima Y
    • Journal Title

      Cell Stem Cell

      Volume: 20 Issue: 6 Pages: 237-238

    • DOI

      10.1016/j.stem.2017.05.004

    • Related Report
      2017 Annual Research Report
  • [Journal Article] History of Pluripotent Stem Cells and Human Naïve Pluripotent Stem Cells2017

    • Author(s)
      Ueda M, Takashima Y
    • Journal Title

      Cytometry Research

      Volume: 27 Issue: 1 Pages: 19-24

    • DOI

      10.18947/cytometryresearch.27.1_19

    • NAID

      130006832438

    • ISSN
      0916-6920, 2424-0664
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discrimination of stem cell status after subjecting cynomolgus monkey pluripotent stem cells to naive conversion.2017

    • Author(s)
      Honda A, Kawano Y, Izu H, Choijookhuu N, Honsho K, Nakamura T, Yabuta Y, Yamamoto T, Takashima Y, Hirose M, Sankai T, Hishikawa Y, Ogura A, Saitou M
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 45285-45285

    • DOI

      10.1038/srep45285

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Pluripotent stem cells in human and common marmoset2018

    • Author(s)
      Yasuhiro Takashima
    • Organizer
      Japan Society for marmoset research, International symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒトナイーブ型多能性幹細胞を用いた再生医療研究とヒト初期発生モデル2018

    • Author(s)
      高島 康弘
    • Organizer
      Molecular Diabetology Coference
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Diiferentiation competence in human naive pluripotente stem cells2017

    • Author(s)
      Yasuhiro Takashima
    • Organizer
      CiRA 2017 International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒトナイーブ型多能性幹細胞と分化2017

    • Author(s)
      高島 康弘
    • Organizer
      第40回 日本分子生物学会(ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 霊長類における初期発生と多能性幹細胞2017

    • Author(s)
      高島 康弘
    • Organizer
      第27回日本サイトメトリー学会学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] DERIVATION OF COMMON MARMOSET PRIMED ES CELLS UNDER HUMAN PRIMED ES CELL CURTURE CONDITION2017

    • Author(s)
      Yasuhiro Takashima
    • Organizer
      15th ISSCR Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒトナイーブ型多能性幹細胞2016

    • Author(s)
      高島 康弘
    • Organizer
      第26回日本サイトメトリー学会学術集会
    • Place of Presentation
      博多
    • Year and Date
      2016-07-23
    • Related Report
      2016 Research-status Report
  • [Presentation] ナイーブ型多能性幹細胞2016

    • Author(s)
      高島 康弘
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2016-05-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Looking at naive and primed human pluripotent stem cells from the point of view of ageing2016

    • Author(s)
      Yasuhiro Takashima
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Santa Fe, USA
    • Year and Date
      2016-05-01
    • Related Report
      2016 Research-status Report
  • [Book] iPS細胞の樹立とあらたな生命科学の開拓2016

    • Author(s)
      高島康弘、山中伸弥
    • Total Pages
      4
    • Publisher
      医学のあゆみ
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] ナイーブ型多能性幹細胞からの原始内胚葉誘導方法2017

    • Inventor(s)
      高島康弘 大久保巧 蝉克憲
    • Industrial Property Rights Holder
      高島康弘 大久保巧 蝉克憲
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-215115
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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