| Project/Area Number |
16K15490
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宮塚 健 順天堂大学, 医学(系)研究科(研究院), 准教授 (60622363)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 糖尿病 / 膵島 / グルカゴン / 膵α細胞 / 細胞分化 / 発生 / 分化 / lineage tracing / 細胞運命 |
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| Outline of Final Research Achievements |
The number of patients with diabetes is still increasing. Therefore, we should establish strategies to solve diabetes-related problems. In addition to pancreatic beta cell abnormality, recently, the abnormality of glucagon producing- alpha cell quality and quantity are also involved in the central pathophysiology of diabetes mellitus, however, there have been less studies regarding alpha cell abnormality compared with beta cell abnormality. As a first step to promote alpha cell biology, here, we tried to establish Glucagon-Timer mice in which a protein, DsRed-E5 (Fluorescent Timer), bound with glucagon promoter shifts its fluorescence spectrum over time. In this mouse, we could observe newly generated alpha cells and isolate those cells and analyzed the gene expression profiles in newly generated alpha cells and mature alpha cells. This mouse will contribute to find the new strategy for diabetes mellitus by reveling alpha cell biology and pathophysiology in future.
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