Explore of a novel molecule regulating Langerhans cells by visualizing method

• Project/Area Number: 16K15495
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: NAKAO KAZUWA 京都大学, 医学研究科, 特任教授 (00172263)
• Co-Investigator(Renkei-kenkyūsha): KANDA HAJIME 京都大学, 大学院医学研究科, 客員研究員 (00724535)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 膵ランゲルハンス島 / ゼブラフィッシュ / アルファ細胞 / ベータ細胞 / デルタ細胞 / 共焦点レーザー顕微鏡 / フローサイトメトリー / セルソーター / 糖尿病 / 生体分子 / 遺伝子 / 細胞・組織 / 動物

Outline of Final Research Achievements

We developed a triple transgenic zebrafish expressing three types of fluorescent reporter protein for alpha cell, beta cell and delta cell of Langerhans islets of pancreas. Our research purpose was elucidation of molecular mechanism regulating volume and ratio of alpha, beta and delta cell in a Langerhans islet. Imaging analysis of 5dpf, 7dpf and 9dpf triple transgenic zebrafish was performed, and we found that the number of alpha cell was significantly increased than beta cells from 5dpf to 7dpf. To analyze molecular mechanism to regulate alpha cell and beta cell ratio, we developed sorting methods to analyze isolated cells of Langerhans of islet by Flow cytometry. Our result and developed methods have a potential to contribute future researches of Langerhans islet cells.

Research Products

• [Journal Article] MiR30-GALNT1/2 Axis-Mediated Glycosylation Contributes to the Increased Secretion of Inactive Human Prohormone for Brain Natriuretic Peptide (proBNP) From Failing Hearts. (2017)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Fujishima A, Oka S, Tsutamoto T, Kinoshita H, Nakao K, Cho K, Inazumi H, Okamoto H, Nishida M, Kato T, Fukushima H, Yamashita JK, Wijnen WJ, Creemers EE, Kangawa K, Minamino N, Nakao K, Kimura T.
  • Journal Title: J Am Heart Assoc. Volume: 6 Issue: 2
  • DOI: 10.1161/jaha.116.003601
  • NAID: 120007100125
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Endothelium-derived C-type natriuretic peptide contributes to blood pressure regulation by maintaining endothelial integrity. (2017)
  • Author(s): Nakao K, Kuwahara K, Nishikimi T, Nakagawa Y, Kinoshita H, Minami T, Kuwabara Y, Yamada C, Yamada Y, Tokudome T, Nagai-Okatani C, Minamino N, Nakao YM, Yasuno S, Ueshima K, Sone M, Kimura T, Kangawa K, Nakao K
  • Journal Title: Hypertension. Volume: 69 Issue: 2 Pages: 286-296
  • DOI: 10.1161/hypertensionaha.116.08219
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Role of leptin in conditioned place preference to high-fat diet in leptin-deficient ob/ob mice. (2017)
  • Author(s): Shimizu Y, Son C, Aotani D, Nomura H, Hikida T, Hosoda K, Nakao K
  • Journal Title: Neuroscience Letters Volume: 640 Pages: 60-63
  • DOI: 10.1016/j.neulet.2017.01.033
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation. Oncogene (2017)
  • Author(s): Otsubo K, Goto H, Nishio M, Kawamura K, Yanagi S, Nishie W, Sasaki T, Maehama T, Nishina H, Mimori K, Nakano T, Shimizu H, Mak TW, Nakao K, Nakanishi Y, Suzuki A
  • Journal Title: Oncogene Volume: 印刷中 Issue: 29 Pages: 4201-4211
  • DOI: 10.1038/onc.2017.58
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ablation of the N-type calcium channel ameliorates diabetic nephropathy with improved glycemic control and reduced blood pressure. (2016)
  • Author(s): Ohno S, Yokoi H, Mori K, Kasahara M, Kuwahara K, Fujikura J, Naito M, Kuwabara T, Imamaki H, Ishii A, Saleem MA, Numata T, Mori Y, Nakao K, Yanagita M, Mukoyama M
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 27192-27192
  • DOI: 10.1038/srep27192
  • NAID: 120007100128
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Overall safety and efficacy of high-dose and low-dose intravenous glucocorticoid therapy in patients with moderate-to-severe active Graves’ ophthalmopathy (2016)
  • Author(s): Ueda-Sakane Y, Kanamoto N, Fushimi Y, Tanaka-Mizuno S, Yasuno S, Miura M, Sone M, Yasoda A, Okada T, Togashi K, Nakao K, Inagaki N
  • Journal Title: Endocrine Journal Volume: 63 Issue: 8 Pages: 703-714
  • DOI: 10.1507/endocrj.EJ16-0038
  • NAID: 130005261383
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Brain-specific natriuretic peptide receptor-B deletion attenuates high-fat diet-induced visceral and hepatic lipid deposition in mice. (2016)
  • Author(s): Yamashita Y, Yamada-Goto N, Katsuura G, Ochi Y, Kanai Y, Miyazaki Y, Kuwahara K, Kanamoto N, Miura M, Yasoda A, Ohinata K, Inagaki N, Nakao K.
  • Journal Title: Peptides Volume: 81 Pages: 38-50
  • DOI: 10.1016/j.peptides.2016.03.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High incorporation of long-chain fatty acids contributes to the efficient production of acylated ghrelin in ghrelin-producing cells. (2016)
  • Author(s): Bando M, Iwakura H, Koyama H, Hosoda H, Shigematsu Y, Ariyasu H, Akamizu T, Kangawa K, Nakao K.
  • Journal Title: FEBS Lett. Volume: 590 Issue: 7 Pages: 992-1001
  • DOI: 10.1002/1873-3468.12132
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Impaired adipogenic capacity in induced pluripotent stem cells from lipodystrophic patients with BSCL2 mutations (2016)
  • Author(s): 1.E. Mori, J. Fujikura, M. Noguchi, K. Nakao, M. Matsubara, M. Sone, D. Taura, T. Kusakabe, K. Ebihara, T. Tanaka, K. Hosoda, K. Takahashi, I. Asaka, N. Inagaki, K. Nakao
  • Journal Title: Metablism Volume: 65 Issue: 4 Pages: 543-556
  • DOI: 10.1016/j.metabol.2015.12.015
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Establishment of Leptin-Responsive Cell Lines from Adult Mouse Hypothalamus. (2016)
  • Author(s): Iwakura H, Dote K, Bando M, Koyama H, Hosoda K, Kangawa K, Nakao K
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0148639-e0148639
  • DOI: 10.1371/journal.pone.0148639
  • NAID: 120005763397
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reevaluation of anti-obesity action of mazindol and elucidation of its effect on the reward system. (2016)
  • Author(s): Aotani D, Son C, Shimizu Y, Nomura H, Hikida T, Kusakabe T, Tanaka T, Miyazawa T, Hosoda K, Nakao K
  • Journal Title: Neuroscience Letters Volume: 633 Pages: 141-145
  • DOI: 10.1016/j.neulet.2016.09.014
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Book] Q&A 生活習慣病の科学 Neo －京都大学健康市民講座 (2016)
  • Author(s): 中尾一和
  • Publisher: 京都大学学術出版会
• [Book] Academic Press (2016)
  • Author(s): 錦見俊雄、桑原宏一郎、中川靖章、寒川賢治、中尾一和
  • Publisher: Endocrinology of the Heart in Health and Disease: Integrated, Cellular, and Molecular Endocrinology of the Heart