Research Project
Grant-in-Aid for Challenging Exploratory Research
We developed a triple transgenic zebrafish expressing three types of fluorescent reporter protein for alpha cell, beta cell and delta cell of Langerhans islets of pancreas. Our research purpose was elucidation of molecular mechanism regulating volume and ratio of alpha, beta and delta cell in a Langerhans islet. Imaging analysis of 5dpf, 7dpf and 9dpf triple transgenic zebrafish was performed, and we found that the number of alpha cell was significantly increased than beta cells from 5dpf to 7dpf. To analyze molecular mechanism to regulate alpha cell and beta cell ratio, we developed sorting methods to analyze isolated cells of Langerhans of islet by Flow cytometry. Our result and developed methods have a potential to contribute future researches of Langerhans islet cells.
All 2017 2016
All Journal Article (11 results) (of which Int'l Joint Research: 11 results, Peer Reviewed: 11 results, Open Access: 9 results, Acknowledgement Compliant: 8 results) Book (2 results)
J Am Heart Assoc.
Volume: 6 Issue: 2
10.1161/jaha.116.003601
120007100125
Hypertension.
Volume: 69 Issue: 2 Pages: 286-296
10.1161/hypertensionaha.116.08219
Neuroscience Letters
Volume: 640 Pages: 60-63
10.1016/j.neulet.2017.01.033
Oncogene
Volume: 印刷中 Issue: 29 Pages: 4201-4211
10.1038/onc.2017.58
Sci Rep
Volume: 6 Issue: 1 Pages: 27192-27192
10.1038/srep27192
120007100128
Endocrine Journal
Volume: 63 Issue: 8 Pages: 703-714
10.1507/endocrj.EJ16-0038
130005261383
Peptides
Volume: 81 Pages: 38-50
10.1016/j.peptides.2016.03.014
FEBS Lett.
Volume: 590 Issue: 7 Pages: 992-1001
10.1002/1873-3468.12132
Metablism
Volume: 65 Issue: 4 Pages: 543-556
10.1016/j.metabol.2015.12.015
PLoS One
Volume: 11 Issue: 2 Pages: e0148639-e0148639
10.1371/journal.pone.0148639
120005763397
Volume: 633 Pages: 141-145
10.1016/j.neulet.2016.09.014