| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
CK7-TVA knock-in ES cells were prepared by inserting a sequence coding for TVA connected to the self-cleaving peptide T2A at the termination codon portion of the CK7 locus. They were found to be capable of gene transfer by RCAS virus. Subsequently, CK7-TVA knock-in ES cells were injected into mouse blastocysts to obtain chimeric mice. Recently, it has been reported that the transcription factor Evi-1 shows hematopoietic stem cell-specific expression in blood cells. Therefore, like CK7, ES cells and mice knocking in TVA at the Evi-1 locus were prepared. These results were published in Scientific reports in 2017.
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