Construction of a new hematopoietic stem cell analysis system with multifunctional TVA system
Project/Area Number |
16K15501
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 造血幹細胞 / RCAS TVA システム / TVA RCAS システム / Krt7 / TVA / RCAS / TVAシステム / 定常状態 |
Outline of Final Research Achievements |
CK7-TVA knock-in ES cells were prepared by inserting a sequence coding for TVA connected to the self-cleaving peptide T2A at the termination codon portion of the CK7 locus. They were found to be capable of gene transfer by RCAS virus. Subsequently, CK7-TVA knock-in ES cells were injected into mouse blastocysts to obtain chimeric mice. Recently, it has been reported that the transcription factor Evi-1 shows hematopoietic stem cell-specific expression in blood cells. Therefore, like CK7, ES cells and mice knocking in TVA at the Evi-1 locus were prepared. These results were published in Scientific reports in 2017.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] 1.An All-Recombinant Protein-Based Culture System Specifically Identifies Hematopoietic Stem Cell Maintenance Factors.2017
Author(s)
Ieyasu A, Ishida R, Kimura T, Morita M, Wilkinson AC, Sudo K, Nishimura T, Ohehara J, Tajima Y, Lai CY, Otsu M, Nakamura Y, Ema H, Nakauchi H, Yamazaki S
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Journal Title
Stem Cell Reports
Volume: 14
Issue: 3
Pages: 500-508
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Depleting dietary valine permits nonmyeloablative mouse hematopoietic stem cell transplantation2016
Author(s)
Taya, Y., Ota, Y., Wilkinson, A. C., Kanazawa, A., Watarai, H., Kasai, M., Nakauchi, H. and Yamazaki, S
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Journal Title
Science
Volume: 354
Issue: 6316
Pages: 1152-1155
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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