| Project/Area Number |
16K15509
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Research Collaborator |
HIROSE Koichi
KONO Kenta
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | IgE / IgA / クラススイッチ / アレルギー / B細胞 / 喘息 / アレルギー・ぜんそく |
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| Outline of Final Research Achievements |
To develop a novel therapeutic strategy which induces class-switch recombination from IgE to IgA in B cells, we investigated whether IgE-producing B cells can be class-switched to IgA-producing B cells by using IgE GFP knock-in mice. CD19+ B cells were isolated from spleen of IgE GFP knock-in mice and cultured under IgE-inducing conditions or IgA-inducing conditions. Four days later, cells were analyzed for the expression of GFP and IgA by FACS. About 9% of B cells were positive for GFP under IgE-inducing conditions, whereas about 4% of B cells were positive for IgA under IgA-inducing conditions. When CD19+ B cells were cultured for 4 days under IgE-inducing conditions and then cultured under IgA-inducing conditions, almost no GFP+ IgA+ cells were detected. These results suggest that the condition we have developed is insufficient to detect class-switch from IgE to IgA. Further investigation is needed to optimize the induction of class-switching to IgA.
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