Analysis of signal transduction mechanisms by chitin through Chitinase-like proteins.

• Project/Area Number: 16K15515
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Saito Hirohisa 国立研究開発法人国立成育医療研究センター, 研究所, 副所長 (40130166)
• Co-Investigator(Renkei-kenkyūsha): NAKAE SUSUMU 東京大学, 医科学研究所, 准教授 (60450409) ; MATSUMOTO KENJI 国立研究開発法人国立成育医療研究センター研究所, 免疫アレルギー感染研究部, 部長 (60181765) ; SUDO KATSUKO 東京医科大学, 医学部, 講師 (50126091)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: キチン / Ｔｈ２ / アレルギー

Outline of Final Research Achievements

Although it is considered that chitin (a major component of the outer shell of house dust mite) can contribute to the induction of allergic responses, it still remains unclear the mechanism of cellular signal transduction by stimulation of chitin. Previously, we identified a Chitinase-like protein as a signal transducer molecule of chitin. In this study therefore, we attempted to identify membrane receptors for the complex of chitin/Chitinase-like protein, but could not clarify the molecule. In the future, we should change the method of screening for identification of the receptors for chitin and Chitinase-like protein complex.

Research Products

• [Int'l Joint Research] SIAF(Switzerland)
• [Int'l Joint Research] Stanford University School of Medicine/Departments of Pathology(米国)
• [Int'l Joint Research] Cambridge/MRC Laboratory of Molecular Biology(英国)
• [Journal Article] Human eosinophils constitutively express a unique serine protease, PRSS33 (2017)
  • Author(s): Toyama S, Okada N, Matsuda A, Morita H, Saito H, Fujisawa T, Nakae S, Karasuyama H, Matsumoto K.
  • Journal Title: Allergology International Volume: 66 Issue: 3 Pages: 463-471
  • DOI: 10.1016/j.alit.2017.01.001
  • NAID: 130005858869
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Yogurt consumption in infancy is inversely associated with atopic dermatitis and food sensitization at 5 years of age: A hospital-based birth cohort study. (2017)
  • Author(s): Shoda T, Futamura M, Yang L, Narita M, Saito H, Ohya Y.
  • Journal Title: J Dermatol Sci Volume: 86 Issue: 2 Pages: 90-6
  • DOI: 10.1016/j.jdermsci.2017.01.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of chronic allergic responses by dampening Bcl6-mediated suppressor activity in memory T helper 2 cells. (2017)
  • Author(s): Ogasawara T, Hatano M, Satake H, Ikari J, Taniguchi T, Tsuruoka N, Watanabe-Takano H, Fujimura L, Sakamoto A, Hirata H, Sugiyama K, Fukushima Y, Nakae S, Matsumoto K, Saito H, Fukuda T, Kurasawa K, Tatsumi K, Tokuhisa T, Arima M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 114 Issue: 5
  • DOI: 10.1073/pnas.1613528114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two-step egg introduction for prevention of egg allergy in high-risk infants with eczema (PETIT): a randomised, double-blind, placebo-controlled trial. (2017)
  • Author(s): Natsume O, Kabashima S, Nakazato J, Yamamoto-Hanada K, Narita M, Kondo M, Saito M, Kishino A, Takimoto T, Inoue E, Tang J, Kido H, Wong GW, Matsumoto K, Saito H, Ohya Y; PETIT Study Team..
  • Journal Title: Lancet Volume: 389 Issue: 10066 Pages: 276-286
  • DOI: 10.1016/s0140-6736(16)31418-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] IL-33 in clinical practice: size matters? (2017)
  • Author(s): Morita H, Nakae S, Saito H, Matsumoto K.
  • Journal Title: J Allergy Clin Immunol Volume: Accepted Issue: 2 Pages: 381-383
  • DOI: 10.1016/j.jaci.2017.03.042
  • Peer Reviewed
• [Journal Article] Preconceptional exposure to oral contraceptive pills and the risk of wheeze, asthma and rhinitis in children (2016)
  • Author(s): Yamamoto-Hanada K, Futamura M, Yang L, Shoda T, Narita M, Kobayashi F, Saito H, Ohya Y.
  • Journal Title: Allergology International Volume: 65 Issue: 3 Pages: 327-331
  • DOI: 10.1016/j.alit.2016.02.012
  • NAID: 130005165868
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Platelets constitutively express IL-33 protein and modulate eosinophilic airway inflammation. (2016)
  • Author(s): Takeda T, Unno H, Morita H, Futamura K, Emi-Sugie M, Arae K, Shoda T, Okada N, Igarashi A, Inoue E, Kitazawa H, Nakae S, Saito H, Matsumoto K, Matsuda A.
  • Journal Title: J Allergy Clin Immunol Volume: In Press Issue: 5 Pages: 1395-1403
  • DOI: 10.1016/j.jaci.2016.01.032
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase in both thymic stromal lymphopoietin and IL-33 levels (2016)
  • Author(s): Shoda T, Matsuda A, Arai K, Shimizu H, Morita H, Orihara K, Okada N, Narita M, Ohya Y, Saito H, Matsumoto K, Nomura I.
  • Journal Title: J Allergy Clin Immunol. Volume: in press Issue: 1 Pages: 299-303
  • DOI: 10.1016/j.jaci.2015.11.042
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Timing of eczema onset and risk of food allergy at 3 years of age: A hospital-based prospective birth cohort study. (2016)
  • Author(s): Shoda T, Futamura M, Yang L, Yamamoto-Hanada K, Narita M, Saito H, Ohya Y.
  • Journal Title: J Dermatol Sci Volume: 84 Issue: 2 Pages: 144-8
  • DOI: 10.1016/j.jdermsci.2016.08.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Recent advances in understanding the roles of vascular endothelial cells in allergic inflammation (2016)
  • Author(s): Shoda T, Futamura K, Orihara K, Emi-Sugie M, Saito H, Matsumoto K, Matsuda A.
  • Journal Title: Allergology International Volume: 65 Issue: 1 Pages: 21-29
  • DOI: 10.1016/j.alit.2015.08.001
  • NAID: 130005290335
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Journal Article] Regulatory roles of mast cells in imuune responses (2016)
  • Author(s): Morita H, Saito H, Matsumoto K, Nakae S.
  • Journal Title: Semin Immunopathol Volume: 38 Issue: 5 Pages: 623-629
  • DOI: 10.1007/s00281-016-0566-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transepidermal water loss measurement during infancy can predict the subsequent development of atopic dermatitis regardless of filaggrin mutations (2016)
  • Author(s): Horimukai K, Morita K, Narita M, Kondo M, Kabashima S, Inoue E, Sasaki T, Niizeki H, Saito H, Matsumoto K, Ohya Y.
  • Journal Title: Allergology International Volume: 65 Issue: 1 Pages: 103-108
  • DOI: 10.1016/j.alit.2015.09.004
  • NAID: 130005290309
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] INTERLEUKIN-33 IN ALLERGIC DISEASES (2016)
  • Author(s): Arae K, Morita H, Matsumoto K, Saito H, Nakae S.
  • Journal Title: Japanese Journal of Allergology Volume: 65 Issue: 10 Pages: 1269-1276
  • DOI: 10.15036/arerugi.65.1269
  • NAID: 130006875586
  • ISSN: 0021-4884, 1347-7935
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A comparison of self-rated and female partner-rated scales in the assessment of paternal prenatal depression. Community Mental Health. (2015)
  • Author(s): Mizuho Konishi, Yoshiyuki Tachibana, Julian Tang, Kenji Takehara, Takahiko Kubo, Keiji Hashimoto, Hiroshi Kitazawa, Hirohisa Saito, Yukihiro Ohya.
  • Journal Title: Community Mental Health. Volume: Published online: 26 August Issue: 8 Pages: 1-6
  • DOI: 10.1007/s10597-015-9931-z
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant