| Project/Area Number |
16K15521
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
Nakamura Tomofumi 熊本大学, 大学院生命科学研究部(医), 研究員 (00772526)
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| Research Collaborator |
Otsu Sachiko
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | HIV-1感染症 / CRISPA-Cas9 / 遺伝子編集 / CRISPR-Cas9 / 蛍光蛋白質 / 逆転写酵素阻害剤 / NHEJ経路 / Cas9による遺伝子編集 / 逆転写酵素阻害剤によるNHEJ促進効果 / エイズ / ゲノム編集 / アデノウイルス随伴ベクター |
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| Outline of Final Research Achievements |
We produced a new assay system for detecting genome editing efficiency of CRISPA-Cas9 (SpCas9) by measuring fluorescent intensity emitting from the fluorescent proteins (Venus) expressed in 293T and COS7 cells, and then investigated and evaluated some compounds that were reported as affecting the genome editing (DNA repair) efficiency. By measuring the Venus fluorescent intensity in the cells using microplate reader and FCM, it was possible to acquire quantitatively the efficiency of Venus genome editing by SpCas9. When we analyzed the compounds of AZT, SCR7, and L755507 reported as genome editing enhancers or inhibitors by using the assay system, significant change of Venus intensity in the presence of the compounds were not detected.
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