| Project/Area Number |
16K15540
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Niigata University (2017)
Hokkaido University (2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
柳 輝希 北海道大学, 医学研究院, 特任助教 (50755973)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | CRISPR/Cas9 / モザイク病変 / モデルマウス / 遺伝性皮膚疾患 |
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| Outline of Final Research Achievements |
In this study, we focus on generation of model mouse with somatic mosaicism of genetic skin disorders. In general, model mice with severe genetic skin disorders such as epidermolysis bullosa and Harlequin ichthyosis cannot survive for a long time period and these mice are not useful for treatment studies. We perform transcutaneous gene-editing for Cas9 nuclease transgenic mice at prenatal or new born period, leading to mosaic model mice that can survive long term. We have succeeded in generating of CRISPR/Cas9 system which can edit targeted genes. And now, we transfect the gene-editing system into the Cas9 nuclease mice.
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