Immunochromatographic method using the salive for rapid diagnosis of bullous pemhigoid

• Project/Area Number: 16K15541
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: SAWAMURA Daisuke 弘前大学, 医学研究科, 教授 (60196334)
• Co-Investigator(Kenkyū-buntansha): 会津 隆幸 弘前大学, 医学研究科, 助教 (00400135) ; 滝吉 典子 弘前大学, 医学部附属病院, 助教 (30568895)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 自己免疫 / 遺伝子 / 皮膚 / 水疱 / ELISA / イムノクロマトグラフィー / ベットサイド / ベッドサイド / 類天疱瘡 / 免疫 / クロマト / 唾液 / 抗体

Outline of Final Research Achievements

Bullous pemphigoid is a skin disease which shows generalized blisters and occurs mostly in the elderly. This disease is characterized by autoantibodies to basement membrane zone and considered as autoimmune blistering disease.　Immunofluorescence study and ELISA analysis using serum usually take long time and we tried to generate a simple and quick method to diagnose bullous pemphigoid. In this study, we demonstrated that saliva is useful for Immunofluorescence study and ELISA analysis of BP180. Furthermore, we development ELISA method and also immunochromatographic method using several recombinant peptides of BP180.

Research Products

• [Journal Article] Multiple milia as an isolated skin manifestation of dominant dystrophic epidermolysis bullosa: Evidence of phenotypic variability. (2017)
  • Author(s): Akasaka E, Nakano H, Takagi Y, Toyomaki Y, Sawamura D
  • Journal Title: Pediatr Dermatol Volume: 34 Issue: 2
  • DOI: 10.1111/pde.13047
  • Peer Reviewed / Open Access
• [Journal Article] The 6-year follow-up of a Japanese patient with silent erythropoietic protoporphyria. (2017)
  • Author(s): Mizawa M, Makino T, Furukawa F, Torai R, Nakano H, Sawamura D, Shimizu T.
  • Journal Title: JAAD Case Rep. Volume: 3 Issue: 3 Pages: 169-171
  • DOI: 10.1016/j.jdcr.2017.01.025
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence of skin infections caused by Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus ?in?Japan, particularly in Ishigaki, Okinawa (2017)
  • Author(s): Takadama S, Nakaminami H, Aoki S, Akashi M, Wajima T, Ikeda M, Mochida A, Shimoe F, Kimura K, Matsuzaki Y, Sawamura D, Inaba Y, Oishi T, Nemoto O, Baba N, Noguchi N
  • Journal Title: J Infect Chemother. Volume: 23 Issue: 11 Pages: 800-803
  • DOI: 10.1016/j.jiac.2017.04.016
  • Peer Reviewed / Open Access
• [Journal Article] C-Terminal Processing of Collagen XVII Induces Neoepitopes for Linear IgA Dermatosis Autoantibodies (2017)
  • Author(s): Toyonaga Ellen、Nishie Wataru、Izumi Kentaro、Natsuga Ken、Ujiie Hideyuki、Iwata Hiroaki、Yamagami Jun、Hirako Yoshiaki、Sawamura Daisuke、Fujimoto Wataru、Shimizu Hiroshi
  • Journal Title: Journal of Investigative Dermatology Volume: 137 Issue: 12 Pages: 2552-2559
  • DOI: 10.1016/j.jid.2017.07.831
  • NAID: 120006540048
  • Peer Reviewed / Open Access
• [Journal Article] Modulation of Stimulator of Interferon Genes (STING) Expression by Interferon-γ in Human Keratinocytes (2017)
  • Author(s): Nishikawa Y, Matsuzaki Y, Kimura K, Rokunohe A, Nakano H, Sawamura D
  • Journal Title: Biochemical Genetics Volume: 56 Issue: 1-2 Pages: 93-102
  • DOI: 10.1007/s10528-017-9832-7
  • Peer Reviewed / Open Access
• [Journal Article] Elevated levels of circulating immunoglobulin E autoantibodies against BP180 and BP230 in an intractable case of bullous pemphigoid. (2016)
  • Author(s): Akasaka E, Hagiwara C, Takiyoshi N, Aizu T, Nakano H, Sawamura D, Ota T
  • Journal Title: J Dermatol Sci. Volume: 84 Issue: 1 Pages: 110-113
  • DOI: 10.1016/j.jdermsci.2016.07.011
  • Peer Reviewed / Open Access
• [Presentation] 最近経験した遺伝性皮膚疾患 (2017)
  • Author(s): 澤村大輔
  • Organizer: 第133回山陰・第29回島根合同開催地方会
  • Invited
• [Presentation] Mutational analysis of porphyria in Japan. (2017)
  • Author(s): Akasaka E, Matsui A, Sawamura D, Nakano H
  • Organizer: 8th Asia and Oceania Conference on Photobiology
  • Int'l Joint Research / Invited
• [Presentation] Peripheral blood mononuclear cells may contain a small amount of mesenchymal stem cells which express type VII collagen. (2017)
  • Author(s): Akssaka E, Nakano H, Sawamura D
  • Organizer: 17th World Dermatology Congress.
  • Int'l Joint Research
• [Presentation] 外科手術を契機に再燃し免疫グロブリン大量静注時に血小板減少をきたした水疱性類天疱瘡 (2017)
  • Author(s): 赤坂英二郎、高橋実か、原 憲司、是川あゆ美、会津隆幸、中野 創、澤村大輔
  • Organizer: 第39回水疱症研究会
• [Presentation] 遺伝性皮膚疾患のトピックス (2016)
  • Author(s): 澤村大輔
  • Organizer: 皮膚科セミナー
  • Place of Presentation: 前橋商工会議所（群馬県・前橋市）
  • Year and Date: 2016-12-15
  • Invited
• [Presentation] 栄養障害型表皮水疱症の遺伝子診断における末梢血単核球のⅦ型コラーゲン発現についての検討 (2016)
  • Author(s): 赤坂英二郎、中野 創、神田由起、鷹木由里子、豊巻由香、澤村大輔
  • Organizer: 第38回水疱症研究会
  • Place of Presentation: ラフレさいたま（埼玉県・さいたま市）
  • Year and Date: 2016-11-29
• [Presentation] 表皮水疱症の最近の話題 (2016)
  • Author(s): 澤村大輔
  • Organizer: 皮膚病態学アフェレシスフォーラム
  • Place of Presentation: ホテルグランヴィア大阪（大阪府・大阪市）
  • Year and Date: 2016-08-04
  • Invited
• [Presentation] 日常診療でみられる遺伝性皮膚疾患、遺伝子診断 (2016)
  • Author(s): 澤村大輔
  • Organizer: 第６回城南皮膚病カンファレンス
  • Place of Presentation: 渋谷エクセルホテル東急（東京都・渋谷区）
  • Year and Date: 2016-07-28
  • Invited
• [Presentation] 稗粒腫を初発症状とし家系内で異なる臨床病型を呈した優性栄養障害型表皮水疱症の1家系例 (2016)
  • Author(s): 赤坂英二郎、中野 創、鷹木由里子、豊巻由香、澤村大輔
  • Organizer: 第115回日本皮膚科学会総会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都（京都府・京都市）
  • Year and Date: 2016-06-03
• [Presentation] 栄養障害型表皮水疱症における末梢血単核球から分離したmRNAを用いたCOL7A1変異検索 (2016)
  • Author(s): 赤坂英二郎、中野 創、神田由紀、鷹木由里子、豊巻由香、澤村大輔
  • Organizer: 第23回分子皮膚科学フォーラム
  • Place of Presentation: ホテルグランヴィア大阪（大阪府・大阪市）
  • Year and Date: 2016-04-15
• [Presentation] Mutational analysis of dystrophic epidermolysis bullosa. (2016)
  • Author(s): Akasaka E, Nakano H, Sawamura D.
  • Organizer: The 13th International Congress of Human Genetics
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2016-04-03
• [Book] 皮膚科研修ノート (2016)
  • Author(s): 澤村大輔、中野 創、宮地良樹、戸倉新樹、山本俊幸、山崎研志、佐山浩二、池田政身、多田譲治、盛山吉弘、伊藤周作、井上雄二、木花いずみ、江川清文、浅田秀夫、日野治子、渡辺大輔、清水 晶、川口博史、望月 隆
  • Total Pages: 712
  • Publisher: 診断と治療社