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Is mitochondrial DNA with normal function necessary for cellular radioresistance?

Research Project

Project/Area Number 16K15571
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionTokyo Medical University

Principal Investigator

Fukumoto Manabu  東京医科大学, 医学部, 特任教授 (60156809)

Co-Investigator(Kenkyū-buntansha) 桑原 義和  東北医科薬科大学, 医学部, 准教授 (00392225)
鈴木 正敏  東北大学, 災害復興新生研究機構, 助教 (60515823)
富田 和男  鹿児島大学, 医歯学域歯学系, 助教 (60347094)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsがん細胞 / 放射線療法 / 化学療法 / 交叉耐性 / ミトコンドリア / ミトコンドリア欠失細胞 / 分割照射 / エピジェネティクス / 放射線耐性 / 代謝
Outline of Final Research Achievements

We analyzed the role of mitochondria (mt) on the phenotype expression of CRR cells that continue to proliferate under fractionated irradiation of 2 Gy/day x-rays. Reactive oxygen species (ROS) derived from mt was detected in the parent cells after acute irradiation with 10 Gy, but was not detected in CRR cells, and the mitDNA copy number and ATP amount were decreased. Using ethidium bromide, mtDNA deleted ρ0 cells could be established from parental ells but was impossible from CRR cells. Only one ρ0 cells could be established from HepG2-CRR to which maintenance irradiation paused. Expression of the antioxidant enzyme group in ρ0 cells showed no specific tendency compared to the parental cells but was rather sensitive to H202. It was suggested that the influx of H 2O2 is accelerated by the decrease in cell membrane potential of ρ0 cells.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Int'l Joint Research] イラン国立血液バンク(イラン)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Clinically relevant radioresistant cell line: a simple model to understand cancer radioresistance.2017

    • Author(s)
      Kuwahara Y, Roudkenar MH, Urushihara Y, Saito Y, Tomita K, Roushandeh AM, Sato T, Kurimasa A, Fukumoto M.
    • Journal Title

      Med Mol Morphol.

      Volume: 50 Issue: 4 Pages: 195-204

    • DOI

      10.1007/s00795-017-0171-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sensitivity of mitochondrial DNA depleted ρ0 cells to H(2)O(2) depends on the plasma membrane status.2017

    • Author(s)
      Tomita K, Kuwahara Y, Takashi Y, Tsukahara T, Kurimasa A, Fukumoto M, Nishitani Y, Sato T.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 490 Issue: 2 Pages: 330-335

    • DOI

      10.1016/j.bbrc.2017.06.044

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM32017

    • Author(s)
      Fujita Takanori、Higashitsuji Hiroaki、Higashitsuji Hisako、Liu Yu、Itoh Katsuhiko、Sakurai Toshiharu、Kojima Takahiro、Kandori Shuya、Nishiyama Hiroyuki、Fukumoto Motoi、Fukumoto Manabu、Shibasaki Koji、Fujita Jun
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 2295-2295

    • DOI

      10.1038/s41598-017-02473-x

    • NAID

      120007129048

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lysophosphatidylcholine export by human ABCA72017

    • Author(s)
      Maiko Tomioka, Yoshinobu Toda, Noralyn B. Manucat, Hiroyasu Akatsu, Manabu Fukumoto, Nozomu Kono, Hiroyuki Arai, Noriyuki Kioka, and Kazumitsu Ueda
    • Journal Title

      Biochimica Biophysica Acta

      Volume: 印刷中 Issue: 7 Pages: 658-665

    • DOI

      10.1016/j.bbalip.2017.03.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Radiation-induced Epstein-Barr virus reactivation in gastric cancer cells with latent EBV infection.2017

    • Author(s)
      Nandakumar A, Uwatoko F, Yamamoto M, Tomita K, Majima H, Akiba S, Koriyma C.
    • Journal Title

      Tumor Biology

      Volume: 39 Issue: 7 Pages: 1-9

    • DOI

      10.1177/1010428317717718

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ミトコントドリア障害細胞における酸化ストレス感受性と細胞膜動態2017

    • Author(s)
      富田和男、桑原義和、高裕子、並河英紀、西谷佳浩、漆原佑介、山西沙祐里、古川みなみ、宮脇正一、栗 政明弘、福本学、佐藤友昭
    • Journal Title

      東北医科薬科大学研究誌

      Volume: 64 Pages: 49-55

    • Related Report
      2017 Annual Research Report
  • [Journal Article] The involvement of mitochondrial membrane potential in cross-resistance between radiation and docetaxel2016

    • Author(s)
      Yoshikazu Kuwahara, Mehryar Habibi Roudkenara, Masatoshi Suzukia, Yusuke Urushihara, Motoi Fukumoto, Yohei Saito, Manabu Fukumoto
    • Journal Title

      Int. J. Radiat. Oncol. Biol. Phys.

      Volume: 66 Issue: 3 Pages: 556-563

    • DOI

      10.1016/j.ijrobp.2016.07.002

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 低線量・低線量率放射線影響は解明できるか2016

    • Author(s)
      福本 学
    • Journal Title

      日本原子力学会誌

      Volume: 58 Pages: 41-44

    • NAID

      130007799956

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 放射線病理学:トロトラスト内部被ばく発がんから福島原発事故被災動物まで2017

    • Author(s)
      福本 学
    • Organizer
      アイソトープ放射線薬学研究会
    • Place of Presentation
      ホテルメトロポリタン仙台(宮城県・仙台市)
    • Year and Date
      2017-03-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 治療耐性がん細胞における過酸化水素耐性メカニズムの解析2017

    • Author(s)
      富田和男、桑原義和、高裕子、並河英紀、塚原飛央、古川みなみ、田中康一、漆原佑介、北中 純一、北中 順惠、栗政明弘、西谷佳浩、宮 脇 正一、西山 信好、竹村 基彦、福本学、佐藤 友昭
    • Organizer
      第70回日本薬理学会西南部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRR細胞における過酸化水素耐性メカニズムの解析2017

    • Author(s)
      富田和男、桑原義和、高裕子、五十嵐健人、漆原佑介、山西沙祐里、宮脇 正一、栗政明弘、西谷佳浩、福本学、佐藤 友昭
    • Organizer
      第3回治療耐性がん細胞研究協議会 セミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] The mechanisms of carcinogenesis induced by internally deposited radionuclides: thinking from Thorortrast cases2016

    • Author(s)
      福本 学
    • Organizer
      Workshop: Mn-56 experiments and the results-Possibility of the small particle effects-
    • Place of Presentation
      南区民文化センター(広島県・広島市)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 被災した家畜や野生動物の線量評価と影響2016

    • Author(s)
      福本 学・鈴木正敏・漆原佑介・林 剛平
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      JMSアステールプラザ(広島県・広島市)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 低線量放射線の健康影響に関する国内関連学会における研究の現状とこれからの連携のあり方2016

    • Author(s)
      福本 学
    • Organizer
      第 53 回アイソトープ・放射線研究発表会
    • Place of Presentation
      東京大学本郷キャンパス(東京都・文京区)
    • Year and Date
      2016-07-06
    • Related Report
      2016 Research-status Report
    • Invited

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Published: 2016-04-21   Modified: 2019-03-29  

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