• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A novel cancer treatment by a combination of irradiation and application of iPS cells

Research Project

Project/Area Number 16K15573
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionUniversity of Tsukuba

Principal Investigator

Tsuboi Koji  筑波大学, 医学医療系, 教授 (90188615)

Co-Investigator(Kenkyū-buntansha) 梨井 康  国立研究開発法人国立成育医療研究センター, 移植免疫研究室, 室長 (60321890)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsRadiotherapy / Dendritic cells / anti-PD1 antibody / abscopal effect / 照射線治療 / 免疫 / 樹状細胞 / 抗PD-1抗体 / 免疫原性細胞死 / がん / 放射線 / 腫瘍免疫 / PD-1
Outline of Final Research Achievements

Local irradiation to tumors may act as antitumor immune adjuvant. We tested whether a combination of local injection of bone marrow or iPS-cells derived dendritic cells (DCs) and intraperitoneal administration of anti-PD-1 antibody after radiotherapy was effective or not to induce antitumor immune response. X-rays and proton beams were used as radiotherapy.
After irradiation to B16 melanoma cells implanted subcutaneously at the left thigh of C57BL/6 mice, DCs were injected to the tumor site 4 times, and anti-PD-1 antibody were administrated 3 times. To examine the abscopal effect, tumor cells were inoculated to the right thigh as metastatic tumors, and only the primary tumors on the left side were treated. As a result, the growth of both the primary and the metastatic tumors were significantly inhibited, extending survival times of mice significantly. There were no significant differences in effects between X-rays and protons, and between BM- and iPS-DCs.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report

Research Products

(22 results)

All 2018 2017 2016

All Journal Article Presentation

  • [Journal Article] Histone deacetylase inhibitor induced radiation sensitization effects on human cancer cells after photon and hadron radiation exposure2018

    • Author(s)
      Ariungerel Gerelchuluun, Junko Maeda, Akira Fujimori et al.
    • Journal Title

      Int J Mol Sci

      Volume: 19(2) Pages: 496-496

    • DOI

      10.3390/ijms19020496

    • NAID

      120006460182

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prognostic analysis of patients who underwent gross total resection of newly diagnosed glioblastoma.2018

    • Author(s)
      Matsuda Masahide、Kohzuki Hidehiro、Ishikawa Eiichi、Yamamoto Tetsuya、Akutsu Hiroyoshi、Takano Shingo、Mizumoto Masashi、Tsuboi Koji、Matsumura Akira
    • Journal Title

      J Clin Neurosci

      Volume: 50 Pages: 172-172

    • DOI

      10.1016/j.jocn.2018.01.009

    • NAID

      120006471227

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Follow-up study of liver metastasis from breast cancer treated by proton beam therapy.2017

    • Author(s)
      Fukumitsu N, Okumura T, Numajiri H, Takizawa D, Ohnishi K, Mizumoto M, Aihara T, Ishikawa H, Tsuboi K, Sakurai H.
    • Journal Title

      Mol Clin Oncol

      Volume: 7 Pages: 56-60

    • DOI

      10.3892/mco.2017.1283

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Registration error of the liver CT using deformable image registration of MIM Maestro and Velocity AI.2017

    • Author(s)
      Fukumitsu N, Nitta K, Terunuma T, Okumura T, Numajiri H, Oshiro Y, Ohnishi K, Mizumoto M, Aihara T, Ishikawa H, Tsuboi K, Sakurai H
    • Journal Title

      BMC Med Imaging

      Volume: 17 Pages: 30-30

    • DOI

      10.1186/s12880-017-0202-z

    • NAID

      120006224638

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assessment of PD-1 positive cells on initial and secondary resected tumor specimens of newly diagnosed glioblastoma and its implications on patient outcome.2017

    • Author(s)
      Miyazaki Tsubasa、Ishikawa Eiichi、Matsuda Masahide、Akutsu Hiroyoshi、Osuka Satoru、Sakamoto Noriaki、Takano Shingo、Yamamoto Tetsuya、Tsuboi Koji、Matsumura Akira
    • Journal Title

      J Neurooncol

      Volume: 133 Pages: 277-285

    • DOI

      10.1007/s11060-017-2451-7

    • NAID

      120006338289

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolic analysis of radioresistant medulloblastoma stem-like clones and potential therapeutic targets.2017

    • Author(s)
      Sun Lue、Moritake Takashi、Ito Kazuya、Matsumoto Yoshitaka、Yasui Hironobu、Nakagawa Hidehiko、Hirayama Aki、Inanami Osamu、Tsuboi Koji
    • Journal Title

      PLoS One.

      Volume: 12 Pages: 1-24

    • DOI

      10.1371/journal.pone.0176162

    • NAID

      120006224679

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-term bioavailability of redox nanoparticles effectively reduces organ dysfunctions and death in whole-body irradiated mice.2017

    • Author(s)
      Feliciano Chitho P.、Tsuboi Koji、Suzuki Kenshi、Kimura Hiroyuki、Nagasaki Yukio
    • Journal Title

      Biomaterials.

      Volume: 129 Pages: 68-82

    • DOI

      10.1016/j.biomaterials.2017.03.011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The abscopal effect induced by in situ-irradiated peripheral tumor cells in a murine GL261 brain tumor model.2017

    • Author(s)
      Zenkoh J, Gerelchuluun A, Wang Y, Miwa Y, Ohno T, Tsuboi K.
    • Journal Title

      Translational cancer research

      Volume: 6(1) Pages: 136-141

    • DOI

      10.21037/tcr.2017.01.32

    • NAID

      120006224159

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A comparative study of dose distribution of pbt, 3d-crt and imrt for pediatric brain tumors2017

    • Author(s)
      Takizawa D, Mizumoto M, Yamamoto T, Oshiro Y, Fukushima H, Fukushima T, Terunuma T, Okumura T, Tsuboi K, Sakurai H.
    • Journal Title

      Radiat Oncol

      Volume: 12(1) Pages: 1-7

    • DOI

      10.1186/s13014-017-0775-2

    • NAID

      120006224008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Long-term outcomes of proton beam therapy in patients with previously untreated hepatocellular carcinoma2017

    • Author(s)
      Fukuda K, Okumura T, Abei M, Fukumitsu N, Ishige K, Mizumoto M, Hasegawa N, Numajiri H, Ohnishi K, Ishikawa H, Tsuboi K, Sakurai H, Hyodo I.
    • Journal Title

      Cancer Sci

      Volume: 108 Pages: 497-503

    • DOI

      10.1111/cas.13145

    • NAID

      120006224020

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Proton beam therapy for liver metastases from gastric cancer2017

    • Author(s)
      Fukumitsu N, Okumura T, Takizawa D, Numajiri H, Ohnishi K, Mizumoto M, Aihara T, Ishikawa H, Tsuboi K, Sakurai H.
    • Journal Title

      Radiat Res

      Volume: - Pages: 357-362

    • DOI

      10.1093/jrr/rrw102

    • NAID

      120006329269

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A prospective multicenter single-arm clinical trial of bevacizumab for patients with surgically untreatable symptomatic brain radiation necrosis2016

    • Author(s)
      Furuse M, Nonoguchi N, Kuroiwa T, Miyamoto S, Arakawa Y, Shinoda J, Miwa K, Iuchi T, K, Houkin K, Terasaka S, Tabei Y, Nakamura H, Nagane M, Sugiyama K, Terasaki M, Abe T, Narita Y, Saito N, Mukasa A, Ogasawara K, Beppu T, Kumabe T, Nariai T, Tsuyuguchi N, Nakatani E, Kurisu S, Naakagawa Y, Miyatake S.
    • Journal Title

      Neurooncol Pract.

      Volume: 0 Pages: 272-280

    • DOI

      10.1093/nop/npv064

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Proton beam therapy with concurrent chemotherapy for glioblastoma multiforme: comparison of nimustine hydrochloride and temozolomide.2016

    • Author(s)
      Mizumoto M, Yamamoto T, Ishikawa E, Matsuda M, Takano S, Ishikawa H, Okumura T, Sakurai H, Matsumura A, Tsuboi K.
    • Journal Title

      J Neurooncol.

      Volume: 130 Pages: 165-170

    • DOI

      10.1007/s11060-016-2228-4

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Methylmercury, an environmental electrophile capable of activation and disruption of the Akt/CREB/Bcl-2 signal transduction pathway in SH-SY5Y cells.2016

    • Author(s)
      Unoki T, Abiko Y, Toyama T, Uehara T, Tsuboi K, Nishida M, Kaji T, Kumagai Y.
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 1-10

    • DOI

      10.1038/srep28944

    • NAID

      120005822867

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hyperfractionated high-dose proton beam radiotherapy for clival chordomas after surgical removal.2016

    • Author(s)
      Hayashi Y, Mizumoto M, Akutsu H, Takano S, Matsumura A, Okumura T, Kawabe T, Zenkoh J, Sakurai H, Tsuboi K.
    • Journal Title

      Br J Radiol.

      Volume: 89(1063) Pages: 1-9

    • DOI

      10.1259/bjr.20151051

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] The abscopal effect in the brain induced by in situ-irradiated peripheral tumor cells in a murine GL261 tumor model.2017

    • Author(s)
      Junko Zenkoh, Ariungerel Gerelchuluun, Yuzi Wang, Lue Sun, Tadao Ohno, Koji Tsuboi
    • Organizer
      The 7th ISRN Conference
    • Place of Presentation
      ホテル双葉(新潟県南魚沼郡)
    • Year and Date
      2017-02-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A combined local treatment with anti-PD-1 antibody and bone-marrow derived DCs after X-ray irradiation to subcutaneous melanoma in a murine model.2017

    • Author(s)
      Wang Yuzi, Koji Tsuboi
    • Organizer
      第18回癌治療増感研究シンポジウム
    • Place of Presentation
      奈良県文化会館(奈良県奈良市)
    • Year and Date
      2017-02-03
    • Related Report
      2016 Research-status Report
  • [Presentation] 局所放射線照射と新規免疫アジュバント“メソポーラスシリカ”を融合したがん放射線免疫療法2016

    • Author(s)
      善光 純子、ゲレルチュルン・アリウンゲレル、王 禹滋、王 秀鵬、十河 友、伊藤 敦夫、大野 忠夫、坪井 康次
    • Organizer
      第13回がんワクチン療法研究会学術集会
    • Place of Presentation
      因島医師会病院(広島県尾道市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report
  • [Presentation] 局所放射線照射と新規免疫アジュバント“メソポーラスシリカ”を融合したがん放射線免疫療法2016

    • Author(s)
      善光 純子、ゲレルチュルン・アリウンゲレル、王 禹滋、大野 忠夫、坪井 康次
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Combination Treatment of Bone Marrow DCs and X-ray Irradiation in a Melanoma Mouse Model.2016

    • Author(s)
      Wang Yuzi、Ariungerel Gerelchuluun、Junko Zenkoh、Xiaokang Li、Koji Tsuboi
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] がんに対する局所的物理療法と全身的免疫療法はお互いを補完するか?2016

    • Author(s)
      坪井 康次、Ariungerel Gerelchuluun、善光 純子、王 禹滋、榮 武二、櫻井 英幸
    • Organizer
      日本ハイパーサーミア学会第33回大会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-09-02
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Proton radiotherapy for adult cases with clival chordoma.2016

    • Author(s)
      Koji Tsuboi
    • Organizer
      7th International Congress of World Federation of Skull Base Surgery.
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2016-06-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi