Genome-wide Screening of Novel Genes Responsible for Tumor Radioresistance
| Project/Area Number |
16K15576
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Kyoto University |
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Principal Investigator |
Harada Hiroshi 京都大学, 放射線生物研究センター, 教授 (80362531)
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| Co-Investigator(Kenkyū-buntansha) |
吉村 通央 京都大学, 医学研究科, 講師 (40597936)
後藤 容子 京都大学, 医学研究科, 特定助教 (60755651)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | がん / 放射線 / 治療抵抗性 / 責任遺伝子 / スクリーニング / 抵抗性 / 原因遺伝子 / 遺伝学的スクリーニング / 放射線抵抗性 |
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| Outline of Final Research Achievements |
Through a genetic screening experiment, we successfully identified novel genes which potentially induce radioresistance of cancer cells. Clonogenic cell survival assay with the use of their expression vector confirmed that some of the genes actually increase radioresistance of cancer cells. We revealed mechanisms of action of one of the genes; it increased the levels of an intracellular antioxidant, GSH, for radioresistance. IHC analysis for clinical tumor samples found positive correlation between the expression levels of the gene product and poor prognosis of cancer patients.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] Hypoxia-inducible factor 1 promotes chemoresistance of lung cancer by inducing carbonic anhydrase IX espression2017
Author(s)
Sowa T, Menju T, Chen-Yoshikawa TF, Takahashi K, Nishikawa S, Nakanishi T, Shikuma K, Motoyama H, Hijiya K, Aoyama A, Sato T, Sonobe M, Harada H, Date H
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Journal Title
Cancer Medicine
Volume: 6
Pages: 288-297
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Evaluation of Tumor-associated Stroma and Its Relationship with Tumor Hypoxia Using Dynamic Contrast-enhanced CT and 18F Misonidazole PET in Murine Tumor Models.2016
Author(s)
Koyasu, S., Tsuji, Y., Harada, H., Nakamoto, Y., Nobashi, T., Kimura, H., Sano, K., Koizumi, K., Hamaji, M., Togashi, K.
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Journal Title
Radiology
Volume: 278
Pages: 734-41
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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