A non-invasive tool for detecting pancreatic tumor-bearing murine odor by C. elegans

• Project/Area Number: 16K15591
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: KAWAMOTO Koichi 大阪大学, 医学系研究科, 特任助教(常勤) (30432470)
• Co-Investigator(Kenkyū-buntansha): 江口 英利 大阪大学, 医学系研究科, 准教授 (90542118) ; 西田 尚弘 大阪大学, 医学系研究科, 助教 (50588118) ; 今野 雅允 大阪大学, 医学系研究科, 寄附講座講師 (80618207) ; 小関 準 大阪大学, 医学系研究科, 特任助教(常勤) (20616669) ; 石井 秀始 大阪大学, 医学系研究科, 特任教授(常勤) (10280736)
• Co-Investigator(Renkei-kenkyūsha): MORI Masaki 大阪大学, 大学院医学系研究科, 教授 (70190999) ; HIROTSU Takaaki 九州大学, 大学院理学研究科, 助教 (70404035) ; SUGIMOTO Masahiro 慶応義塾大学, 政策メディア研究科, 特任准教授 (30458963)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵癌 / 早期診断 / 綿虫 / 嗅覚レセプター / スクリーニング / 線虫 / 聴覚レセプター

Outline of Final Research Achievements

Diagnosis of pancreatic ductal adenocarcinoma is associated with dismal prognosis despite current best therapies, including surgery, chemoradiation, and immunotherapy. Previous studies reported that wild-type C. Elegans displayed attractive chemotaxis towards human urine from cancer patients but avoided control urine from healthy volunteers. In this study, our research group demonstrated that C. Elegans induces chemotactic properties to the urine from pancreatic tumor-bearing mice. Furthermore, urine from c-Met knockout has minimal effect to these chemotactic properties. These results suggest that urine from tumor-bearing mice could provide a new strategy to detect and study disease-associated scents in the future.

Research Products

• [Journal Article] Enzymes of the one-carbon folate metabolism as anticancer targets predicted by survival rate analysis (2018)
  • Author(s): Koseki Jun、Konno Masamitsu、Asai Ayumu、Colvin Hugh、Kawamoto Koichi、Nishida Naohiro、Sakai Daisuke、Kudo Toshihiro、Satoh Taroh、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 303-303
  • DOI: 10.1038/s41598-017-18456-x
  • Peer Reviewed / Open Access
• [Journal Article] Computational trans-omics approach characterised methylomic and transcriptomic involvements and identified novel therapeutic targets for chemoresistance in gastrointestinal cancer stem cells (2018)
  • Author(s): Konno Masamitsu、Matsui Hidetoshi、Koseki Jun、Asai Ayumu、Kano Yoshihiro、Kawamoto Koichi、Nishida Naohiro、Sakai Daisuke、Kudo Toshihiro、Satoh Taroh、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 899-899
  • DOI: 10.1038/s41598-018-19284-3
  • Peer Reviewed / Open Access
• [Journal Article] The one-carbon metabolism pathway highlights therapeutic targets for gastrointestinal cancer (Review) (2017)
  • Author(s): Konno Masamitsu、Asai Ayumu、Kawamoto Koichi、Nishida Naohiro、Satoh Taroh、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Int. J. Oncol. Volume: 50 Issue: 4 Pages: 1057-1063
  • DOI: 10.3892/ijo.2017.3885
  • Peer Reviewed / Open Access
• [Journal Article] Micro-RNA-130a-3p Regulates Gemcitabine?Resistance via PPARG in Cholangiocarcinoma (2017)
  • Author(s): Asukai Kei、Kawamoto Koichi、Eguchi Hidetoshi、Konno Masamitsu、Asai Ayumu、Iwagami Yoshifumi、Yamada Daisaku、Asaoka Tadafumi、Noda Takehiro、Wada Hiroshi、Gotoh Kunihito、Nishida Naohiro、Satoh Taroh、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Ann. Surg. Oncol. Volume: 24 Issue: 8 Pages: 2344-2352
  • DOI: 10.1245/s10434-017-5871-x
  • Peer Reviewed / Open Access
• [Journal Article] Hypoxia stimulates the cytoplasmic localization of oncogenic long noncoding RNA LINC00152 in colorectal cancer (2017)
  • Author(s): Nishizawa Yujiro、Konno Masamitsu、Asai Ayumu、Koseki Jun、Kawamoto Koichi、Miyoshi Norikatsu、Takahashi Hidekazu、Nishida Naohiro、Haraguchi Naotsugu、Sakai Daisuke、Kudo Toshihiro、Hata Taishi、Matsuda Chu、Mizushima Tsunekazu、Satoh Taroh、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Int. J. Oncol. Volume: 52 Pages: 453-460
  • DOI: 10.3892/ijo.2017.4218
  • Peer Reviewed / Open Access
• [Journal Article] Oncogene c-Myc promotes epitranscriptome m6A reader YTHDF1 expression in colorectal cancer (2017)
  • Author(s): Nishizawa Yujiro、Konno Masamitsu、Asai Ayumu、Koseki Jun、Kawamoto Koichi、Miyoshi Norikatsu、Takahashi Hidekazu、Nishida Naohiro、Haraguchi Naotsugu、Sakai Daisuke、Kudo Toshihiro、Hata Taishi、Matsuda Chu、Mizushima Tsunekazu、Satoh Taroh、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Oncotarget. Volume: 9 Issue: 7 Pages: 7476-7486
  • DOI: 10.18632/oncotarget.23554
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial pyruvate carrier modulates the epithelial-mesenchymal transition in cholangiocarcinoma (2017)
  • Author(s): Ohashi Tomofumi、Eguchi Hidetoshi、Kawamoto Koichi、Konno Masamitsu、Asai Ayumu、Colvin Hugh、Ueda Yuji、Takaoka Hirofumi、Iwagami Yoshifumi、Yamada Daisaku、Asaoka Tadafumi、Noda Takehiro、Wada Hiroshi、Gotoh Kunihito、Kobayashi Shogo、Koseki Jun、Satoh Taroh、Ogawa Kazuhiko、Doki Yuichiro、Mori Masaki、Ishii Hideshi
  • Journal Title: Oncol. Rep. Volume: 39 Pages: 1276-1282
  • DOI: 10.3892/or.2017.6172
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic adaptation to nutritional stress in human colorectal cancer. (2016)
  • Author(s): Miyo, M., Konno, M., Nishida, N. Sueda, T., Noguchi, K., Matsui, H., Colvin, H., Kawamoto, K., Koseki, J., Haraguchi, N., Nishimura, J., Hata, T., Gotoh, N., Mastuda, F., Saatoh, T., Mizushima, T., Shimizu, H., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 38415-38415
  • DOI: 10.1038/srep38415
  • Peer Reviewed / Open Access
• [Journal Article] Oncometabolite D-2-hydroxyglurate directly induces epithelial-mesenchymal transition and is associated with distant metastasis in colorectal cancer. (2016)
  • Author(s): Colvin, S. H., Nishida, N., Konno, M., Haraguchi, N., Takahashi, H., Nishimura, J., Hata, T., Kawamoto, K. Asai, A., Tsunekuni, K., Koseki, J., Mizushima, T., Satoh, T., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Sci. Rep Volume: 6 Issue: 1 Pages: 36289-36289
  • DOI: 10.1038/srep36289
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 胆管がん微少環境下における炎症性サイトカイン発現の臨床的意義に関する検討 (2017)
  • Author(s): 川本弘一、江口英利、飛鳥井慶、今野雅允、西田尚弘、小関準、浅井歩、岩上佳史、山田大作、浅岡忠史、野田剛広、和田浩志、後藤邦仁、佐藤太郎、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第26回日本がん転移学会学術集会・総会
• [Presentation] 長鎖ノンコーディングRNAの大腸癌におけるエピゲノム制御 (2017)
  • Author(s): 西田尚弘、Jana Ferdin、今野雅允、小関準、川本弘一、 浅井歩、George Calin、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第76回日本癌学会学術総会