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Establishment of a system to measure DNA repair activity using circulating tumor cells: the key to predict sensitivity to PARP inhibitors

Research Project

Project/Area Number 16K15594
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionNagasaki University

Principal Investigator

MITSUTAKE Norisato  長崎大学, 原爆後障害医療研究所, 准教授 (50404215)

Co-Investigator(Kenkyū-buntansha) 中沢 由華  名古屋大学, 環境医学研究所, 助教 (00533902)
Research Collaborator TANAKA Aya  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords乳癌 / DNA修復 / 乳がん
Outline of Final Research Achievements

We have developed a method to isolate and proliferate primary breast cancer cells from clinical tissue samples. Using this method, we have made frozen stocks of the cells isolated from cases with high probability of familial/hereditary breast cancers.
Although isolating circulating tumor cells (CTCs) was possible, it was unfortunately difficult to propagate them. Because of that, we focused on the study using cells isolated from cancer tissues.
We have developed a method by which DNA repair activity by homologous recombination can be measured. In this method, residual DNA damage was quantified at the G2 phase using nuclear and phospho-H2AX staining.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (1 results)

All 2016

All Presentation (1 results)

  • [Presentation] ゲノム不安定性を示す遺伝性疾患群の病態解析と新規疾患責任遺伝子変異探索2016

    • Author(s)
      中沢由華
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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