Single cell heterogeneity analysis of endothelial and mesenchymal cells

• Project/Area Number: 16K15597
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Yokohama City University
• Principal Investigator: SEKINE Keisuke 横浜市立大学, 医学部, 助教 (00323569)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: iPS細胞 / 再生医療 / シングルセルゲノミクス / 移植・再生医療 / シングルセル解析 / RNAシークエンス

Outline of Final Research Achievements

Comprehensive gene expression analysis was performed on human umbilical cord-derived vascular endothelial cells and human bone marrow mesenchymal cells at the single cell level. Gene expression data on vascular endothelial cells and mesenchymal cells in the iPSC- liver bud was also obtained. Genes expressed in each cell population were analyzed. Furthermore, it was revealed that genes involved in cell-cell interaction in iPSC liver bud. We also analyzed heterogeneity in each cell population. These findings are useful information for quality evaluation in the process of manufacturing human iPSC- liver bud, and for future improvement of iPSC- liver bud quality and safety.

Research Products

• [Int'l Joint Research] University of Alberta(カナダ)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] 台湾大学(台灣)
• [Int'l Joint Research] MaxPlanck Institute(Germany)
• [Journal Article] Self-Condensation Culture Enables Vascularization of Tissue Fragments for Efficient Therapeutic (2018)
  • Author(s): Yoshinobu Takahashi, Keisuke Sekine, Tatsuya Kin, Takanori Takebe, Hideki Taniguchi
  • Journal Title: Cell Reports Volume: 6 Issue: 6 Pages: 1620-1629
  • DOI: 10.1016/j.celrep.2018.03.123
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids. (2018)
  • Author(s): Zhang R-R, Koido M, Tadokoro T, Ouchi R, Matsuno T, Ueno Y, Sekine K, Takebe T*, Taniguch H
  • Journal Title: Stem Cell Reports Volume: 10 Issue: 3 Pages: 780-793
  • DOI: 10.1016/j.stemcr.2018.01.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multilineage communication regulates human liver bud self-organization from pluripotency. (2017)
  • Author(s): Camp JG, Sekine K, Gerber T, Loeffler-Wirth H, Binder H, Gac M, Kanton S, Kageyama J, Damm G, Seehofer D, Belicova L, Bickle M, Barsacchi R, Okuda R, Yoshizawa E, Kimura M, Ayabe H, Taniguchi H, Takebe T*, Treutlein B
  • Journal Title: Nature Volume: 546 Issue: 7659 Pages: 533-534
  • DOI: 10.1038/nature22796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chitosan promotes cancer progression and stem cell properties in association with Wnt signaling in colon and hepatocellular carcinoma cells (2017)
  • Author(s): Chang Po-Hsiang、Sekine Keisuke、Chao Hsiao-Mei、Hsu Shan-hui、Chern Edward
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 45751-45751
  • DOI: 10.1038/srep45751
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Liver Regeneration Using Cultured Liver Bud (2017)
  • Author(s): Sekine K, Takebe T, Taniguchi H.
  • Journal Title: Methods Mol Biol Volume: 1597 Pages: 207-216
  • DOI: 10.1007/978-1-4939-6949-4_15
  • ISBN: 9781493969470, 9781493969494
  • Peer Reviewed / Open Access
• [Journal Article] Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism. (2017)
  • Author(s): Koike H, Zhang RR, Ueno Y, Sekine K, Zheng YW, Takebe T, Taniguchi H.
  • Journal Title: Development Volume: 144(6) Pages: 1018-1024
  • DOI: 10.1242/dev.143032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ヒトiPSC肝芽の臨床応用に向けた安全性評価手法の確立 (2018)
  • Author(s): 濱田 侑希、関根 圭輔、都築 周作、小林 達也、上野 康晴、武部 貴則、谷口 英樹
  • Organizer: 第17回日本再生医療学会総会
• [Presentation] 血管化膵島移植による革新的糖尿病治療法の確立 (2017)
  • Author(s): 高橋禎暢、武部貴則、小池直人、関根圭輔、谷口英樹
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター(宮城)
  • Year and Date: 2017-03-07
• [Presentation] 癌微小環境を模倣した患者由来ヒト膵がんオルガノイドの創出 (2017)
  • Author(s): 奥田 諒、関根 圭輔、上野康晴 康晴、佐藤 準也、高橋 正希、井上 達也、金子 千夏、碓井 彩華、谷口 英樹 / 横浜市大・医・臓器再生医学
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 腫瘍微小環境を模倣するヒト膵癌オルガノイドは薬剤耐性を示す (2017)
  • Author(s): 関根 圭輔、奥田 諒、井上 達也、金子 千夏、碓井 彩華、上野 康晴、谷口 英樹
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 腫瘍微小環境を伴う膵癌オルガノイドは薬剤耐性を示す (2017)
  • Author(s): 碓井 彩華、奥田 諒、関根 圭輔、上野 康晴、佐藤 準也、高橋 正希、井上 達也、金子 千夏、谷口 英樹
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 腫瘍微小環境を伴う膵癌オルガノイドはin vitroで治療抵抗性を示す (2017)
  • Author(s): 金子 千夏 、奥田 諒、関根 圭輔、上野 康晴、佐藤 準也、高橋 正希、井上 達也、碓井 彩華、谷口 英樹
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 微小環境を伴うヒト膵オルガノイドの再構成に基づく新規膵癌薬剤評価系の構築 (2017)
  • Author(s): 上野 康晴、奥田 諒、金子 千夏、碓井 彩華、関根 圭輔、谷口 英樹
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] シングルセルレベルでのiPSC肝芽構成細胞のポピュレーションヘテロジェネイティ解析 (2016)
  • Author(s): 関根 圭輔、J. Gray Camp、Barbara Treutlein、武部 貴則、谷口 英樹
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2016-11-30
• [Presentation] 腫瘍微小環境を模倣するヒト膵癌オルガノイドの創出 (2016)
  • Author(s): 奥田諒、関根圭輔、上野康晴、佐藤準也、高橋正希、濱中香織、谷口英樹
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2016-11-30
• [Presentation] 臓器形成におけるヘテロジェネイティの理解と制御 (2016)
  • Author(s): 武部 貴則、関根 圭輔、谷口 英樹
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2016-11-30
• [Presentation] 腫瘍微小環境を模倣するヒト膵癌オルガノイドの創出 (2016)
  • Author(s): 奥田 諒、高橋 正希、佐藤 準也、関根 圭輔、上野 康晴、谷口 英樹
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2016-10-06
• [Presentation] De novo generation of organ buds from stem cells (2016)
  • Author(s): Keisuke Sekine
  • Organizer: 21st NAT Congress
  • Place of Presentation: Nantes, France
  • Year and Date: 2016-06-09
  • Int'l Joint Research / Invited
• [Book] Organ Regeneration Based on Developmental Biology (2017)
  • Author(s): Keisuke Sekine, Takanori Takebe, Hideki Taniguchi
  • Total Pages: 257
  • Publisher: Springer book
  • ISBN: 9789811037665