• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic research for development of the DDS system considering tissue and intracellular drug distribution

Research Project

Project/Area Number 16K15600
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionNational Cancer Center Japan

Principal Investigator

NISHIDA TOSHIROU  国立研究開発法人国立がん研究センター, 中央病院, 病院長 (40263264)

Co-Investigator(Kenkyū-buntansha) 安永 正浩  国立研究開発法人国立がん研究センター, 先端医療開発センター, ユニット長 (80450576)
Research Collaborator KUROKAWA Kazuo  理化学研究所, 光量子工学研究領域 (40333504)
MANABE Shino  理化学研究所, 伊藤細胞制御化学研究室 (60300901)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsDrug-Delivery System / 分子標的治療薬 / イマチニブ / 消化管間質腫瘍 / 細胞株 / DDS / 薬物動態 / 薬剤動態
Outline of Final Research Achievements

It is important to develop DDS which efficiently and specifically delivers anti-cancer agents to cancer cells. The purpose of this research is to develop basic technology to measure accurately drug distribution at cellular and organellar levels using GIST-imatinib as a model. We have studied using xenograft model and GIST cell lines. Imatinib distribution in tumor tissues was measured with mass-microscopes whose sensitivity was enhanced nearly 10-hold.
Although tumor vascular density greatly decreased after imatinib therapy, there was no change in tumor tissue distribution of imatinib in xenograft models. Intracellular imatinib distribution was measured by fluorescent-tagged imatinib, Turn-ON method, and using anti-imatinib antibody. When imatinib entered into tumor cells, it rapidly accumulated mainly in the secondary lysosome, and partly distributed ER-like structures. Taken together, imatinib was accumulated mainly in lysosome and partly in ER-like structures of tumor cells.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results) Book (1 results)

  • [Journal Article] Clinicopathological features and prognosis of primary GISTs with tumor rupture in the real world2018

    • Author(s)
      Nishida T, Cho H, Hirota S, Masuzawa T, Chiguchi G, Tsujinaka T, the members of the Kinki GIST Study Group.
    • Journal Title

      Ann Surg Oncol

      Volume: epub

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SOCS1 gene therapy has antitumor effects in imatinib-resistant gastrointestinal stromal tumor cells through FAK/PI3K signaling.2018

    • Author(s)
      Sugase T, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Hiramatsu K, Nishida T, Hirota S, Saito Y, Tanaka K, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Nakajima K, Hanasaki K, Kishimoto T, Mori M, Doki Y, Naka T.
    • Journal Title

      Gastric Cancer.

      Volume: epub Issue: 6 Pages: 968-976

    • DOI

      10.1007/s10120-018-0822-1

    • NAID

      40021694606

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Asian consensus guidelines for GIST: What is the same and what is different from global guidelines.2018

    • Author(s)
      Nishida T.
    • Journal Title

      Transl Gastroenterol Hepatol.

      Volume: 3 Pages: 11-11

    • DOI

      10.21037/tgh.2018.01.07

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Downregulation of miR-186 is associated with metastatic recurrence of gastrointestinal stromal tumors.2017

    • Author(s)
      Niinuma T, Kai M, Kitajima H, Yamamoto E, Harada T, Maruyama R, Nobuoka T, Kanda T, Hasegawa T, Tokino T, Sugai T, Shimomura Y, Nakase H, Suzuki H
    • Journal Title

      Oncology Letters

      Volume: 14 Pages: 5703-5710

    • DOI

      10.3892/ol.2017.6911

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of antibody drug conjugates using DDS and molecular imaging.2017

    • Author(s)
      Yasunaga M, Manabe S, Tsuji A, Furuta M, Ogata K, Koga Y, Saga T, Matsumura Y.
    • Journal Title

      Bioengineering

      Volume: 4 Issue: 3 Pages: 78-78

    • DOI

      10.3390/bioengineering4030078

    • NAID

      120006497386

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical outcomes of patients with gastrointestinal stromal tumor in phase I clinical trials2016

    • Author(s)
      Nagatani Y, Shitara K, Bando H, Kuboki Y, Okamoto W, Kojima T, Yoshino T, Nishida T, Ohtsu A, Doi T.
    • Journal Title

      BMC Cancer

      Volume: 16 Issue: 1 Pages: 889-889

    • DOI

      10.1186/s12885-016-2939-0

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinicopathological characteristics, surgery, and survival outcomes of duodenal gastrointestinal stromal tumors.2016

    • Author(s)
      Sugase T, Takahashi T, Nakajima K, Hirota H, Masuzawa T, Nishida T, Kimura Y, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Takiguchi S, Mori M, Doki Y.
    • Journal Title

      Digestion

      Volume: 94 Issue: 11 Pages: 30-36

    • DOI

      10.1002/ijc.30666

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Asian Consensus Guidelines for the Diagnosis and Management of Gastrointestinal Stromal Tumor.2016

    • Author(s)
      Koo DH, Ryu MH, Kim KM, Yang HK, Sawaki A, Hirota S, Zheng J, Zhang B, Tzen CY, Yeh CN, Nishida T, Shen L, Chen LT, Kang YK.
    • Journal Title

      Cancer Res Treat

      Volume: 48 Issue: 4 Pages: 1155-1166

    • DOI

      10.4143/crt.2016.187

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gastrointestinal stromal tumors in Japanese patients with neurofibromatosis type I.2015

    • Author(s)
      Nishida T, Tsujimoto M, Takahashi T, Hirota S, Blay J-V, Wataya-Kaneda M.
    • Journal Title

      J Gastroenterol

      Volume: s00535 Issue: 6 Pages: 1132-6

    • DOI

      10.1007/s00535-015-1132-6

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Current treatment and perspectives of GIST in Japan2018

    • Author(s)
      第90回日本胃癌学会総会
    • Organizer
      Korean GIST Study Group Symposium 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 難治性がんを標的にしたイムノPET イメージング法の開発2017

    • Author(s)
      安永正浩、辻厚至、高島大輝、佐賀恒夫、松村保広
    • Organizer
      第11回日本分子イメージング学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Visualisation of EPR effect and active targeting by using microscopic mass spectrometry.2017

    • Author(s)
      Masahiro Yasunaga, Masaru Furuta, Koretsugu Ogata, Yuki Fujiwara, Yoshikazu Koga and Yasuhiro Matsumura
    • Organizer
      American Association for Cancer Research Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of antibody-drug conjugate by utilizing molecular imaging2017

    • Author(s)
      Masahhiro Yasunaga
    • Organizer
      Federation of Asian Societies for Molecular Imaging.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Optional or Optimal?: Laparoscopic Surgery versus Open Resection in Local GIST2017

    • Author(s)
      Toshirou Nishida
    • Organizer
      12th International Gastric Cancer Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 食道非上皮性腫瘍の治療2016

    • Author(s)
      西田俊朗
    • Organizer
      第70回日本食道学会学術集会
    • Place of Presentation
      ザ・プリンス パークタワー東京(東京都港区)
    • Year and Date
      2016-07-05
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Registry and Biobank for Rare Cancer in Japan:Gastrointestinal Stromal Tumor,Sarcoma and Neuroendocrine Tumor2016

    • Author(s)
      Toshirou Nishida
    • Organizer
      2nd Taiwan-Japan AROworkshop
    • Place of Presentation
      Taipei (Taiwan)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Laparoscopic resection for SET near pyloric ring or GEJ2016

    • Author(s)
      Toshirou Nishida
    • Organizer
      KINGCA WEEK 2016
    • Place of Presentation
      Seoul (Korea)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 抗体薬物複合体の研究開発における分子イメージングの有用性 PHARMSTAGE2017

    • Author(s)
      安永正浩、眞鍋史乃、辻厚至、古田大、 緒方是嗣、古賀宣勝、藤原悠起、佐賀恒夫、松村保広
    • Total Pages
      70
    • Publisher
      技術情報協会
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi