• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Characterization of the esophageal carcinogenesis-promoting molecular switch existing inside the isoform of RNA-binding protein

Research Project

Project/Area Number 16K15618
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionThe University of Tokushima

Principal Investigator

IMOTO Issei  徳島大学, 大学院医歯薬学研究部(医学系), 教授 (30258610)

Co-Investigator(Renkei-kenkyūsha) MASUDA Kiyoshi  徳島大学, 大学院医歯薬学研究部, 准教授 (00457318)
OTSUJI Eigo  京都府立医科大学, 医学(系)研究科, 教授 (20244600)
TANGOKU Akira  徳島大学, 大学院医歯薬学研究部, 教授 (10197593)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsRNA結合蛋白 / TIA1 / アイソフォーム / 天然変性領域 / 蛋白リン酸化 / 癌促進作用 / 細胞内局在 / 分子スイッチ / RNA結合タンパク / TIA1a / 癌
Outline of Final Research Achievements

TIA1a is the isoform of TIA1, one of the RNA-binding protein (RBP), having a serine/threonine-rich intrinsically disordered region (IDR) and promoting esophageal squamous cell carcinoma (ESCC). Through changing amino acid of candidate phosphorylation sites around the TIA1a specific region within IDR, subcellular localization of TIA1a and cell growth were altered in ESCC cells. Blocking synthetic peptides inhibited TIA1a cytoplasmic localization. CHK1 was identified as candidate kinase contributing to TIA1a cytoplasmic localization. In addition, TIA1a bound 3'UTR of candidate TIA1a-binding mRNAs in the cytoplasm with other RBPs and regulated mRNA/protein levels of those targets jointly or competitively. These results demonstrated that the phosphorylation of specific sites within IDR may work as a molecular switch to promote cytoplasmic localization of TIA1a and accelerate its cancer-promoting function through binding and regulating target molecules in the cytoplasm of ESCC cells.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (40 results)

All 2018 2017 2016 Other

All Journal Article (17 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 17 results,  Open Access: 15 results,  Acknowledgement Compliant: 5 results) Presentation (19 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (2 results) Remarks (2 results)

  • [Journal Article] A rare male patient with classic Rett syndrome caused by MeCP2_e1 mutation2018

    • Author(s)
      Tokaji Narumi、Ito Hiromichi、Kohmoto Tomohiro、Naruto Takuya、Takahashi Rizu、Goji Aya、Mori Tatsuo、Toda Yoshihiro、Saito Masako、Tange Shoichiro、Masuda Kiyoshi、Kagami Shoji、Imoto Issei
    • Journal Title

      American Journal of Medical Genetics Part A

      Volume: 176 Issue: 3 Pages: 699-702

    • DOI

      10.1002/ajmg.a.38595

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of pathogenic germline variants detected by multigene sequencing in unselected Japanese patients with ovarian cancer2017

    • Author(s)
      Hirasawa Akira、Imoto Issei、Naruto Takuya、Akahane Tomoko、Yamagami Wataru、Nomura Hiroyuki、Masuda Kiyoshi、Susumu Nobuyuki、Tsuda Hitoshi、Aoki Daisuke
    • Journal Title

      Oncotarget

      Volume: 8 Issue: 68 Pages: 112258-112267

    • DOI

      10.18632/oncotarget.22733

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] KH-type splicing regulatory protein is involved in esophageal squamous cell carcinoma progression2017

    • Author(s)
      Y. Fujita, K. Masuda, J. Hamada, K. Shoda, T. Naruto, S. Hamada, Y. Miyakami, T. Kohmoto, M. Watanabe, R. Takahashi, S. Tange, M. Saito, Y. Kudo, H. Fujiwara, D. Ichikawa, A. Tangoku, E. Otsuji, I. Imoto,
    • Journal Title

      Oncotarget.

      Volume: 8 Issue: 60 Pages: 101130-101145

    • DOI

      10.18632/oncotarget.20926

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Frequent silencing of RASSF1A by DNA methylation in thymic neuroendocrine tumours2017

    • Author(s)
      Kajiura K, Takizawa H, Morimoto Y, Masuda K, Tsuboi M, Kishibuchi R, Wusiman N, Sawada T, Kawakita N, Toba H, Yoshida M, Kawakami Y, Naruto T, Imoto I, Tangoku A, Kondo K
    • Journal Title

      Lung Cancer

      Volume: 111 Pages: 116-123

    • DOI

      10.1016/j.lungcan.2017.05.019

    • NAID

      120006539750

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel CLCN7 compound heterozygous mutations in intermediate autosomal recessive osteopetrosis2017

    • Author(s)
      N. Okamoto, T. Kohmoto, T. Naruto, K. Masuda, T. Komori, I. Imoto
    • Journal Title

      Hum Genome Var

      Volume: 4 Issue: 1 Pages: 1-4

    • DOI

      10.1038/hgv.2017.36

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The first Japanese patient with mandibular hypoplasia, deafness, progeroid features and lipodystrophy diagnosed via POLD1 mutation detection2017

    • Author(s)
      Okada A, Kohmoto T, Naruto T, Yokota I, Kotani Y, Shimada A, Miyamoto Y, Takahashi R, Goji A, Masuda K, Kagami S, Imoto I
    • Journal Title

      Hum Genome Var

      Volume: 4 Issue: 1 Pages: 17031-17031

    • DOI

      10.1038/hgv.2017.31

    • NAID

      120006955877

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction of a combinatorial pipeline using two somatic variant  calling  methods  for whole exome sequence data of gastric cancer2017

    • Author(s)
      Kohmoto T, Masuda K, Naruto T, Tange S, Shoda K, Hamada J, Saito M, Ichikawa D, Tajima A, Otsuji E, Imoto I.
    • Journal Title

      The Journal of Medical Investigation

      Volume: 64 Issue: 3.4 Pages: 233-240

    • DOI

      10.2152/jmi.64.233

    • NAID

      130006105184

    • ISSN
      1343-1420, 1349-6867
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A case with concurrent duplication, triplication, and uniparental isodisomy at 1q42.12-qter supporting microhomology-mediated break-induced replication model for replicative rearrangements2017

    • Author(s)
      Kohmoto T, Okamoto N, Naruto T, Murata C, Ouchi Y, Fujita N, Inagaki H, Satomura S, Okamoto N, Saito M, Masuda K, Kurahashi H, Imoto I
    • Journal Title

      Mol Cytogenet

      Volume: 10 Issue: 1 Pages: 15-15

    • DOI

      10.1186/s13039-017-0316-6

    • NAID

      120006937633

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical utility of circulating cell-free Epstein-Barr virus DNA in patients with gastric cancer.2017

    • Author(s)
      Shoda K, Ichikawa D, Fujita Y, Masuda K, Hiramoto H, Hamada J, Arita T, Konishi H, Kosuga T, Komatsu S, Shiozaki A, Okamoto K, Imoto I, Otsuji E.
    • Journal Title

      Oncotarget

      Volume: 17 Issue: 17 Pages: 28796-28804

    • DOI

      10.18632/oncotarget.15675

    • NAID

      120006306639

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma2017

    • Author(s)
      K.Kajiura, K.Masuda, T.Naruto, T.Kohmoto, M.Watanabe, M.Tsuboi, H.Takizawa, K.Kondo, A.Tangoku, I.Imoto
    • Journal Title

      Oncotarget

      Volume: 8 Issue: 2 Pages: 2890-2905

    • DOI

      10.18632/oncotarget.13761

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-first approach diagnosed Cabezas syndrome via novel CUL4B mutation detection.2017

    • Author(s)
      Okamoto N, Watanabe M, Naruto T, Matsuda K, Kohmoto T, Saito M, Masuda K, Imoto I.
    • Journal Title

      Hum Genome Var

      Volume: 4 Issue: 1 Pages: 16045-16045

    • DOI

      10.1038/hgv.2016.45

    • NAID

      120006955876

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A 590 kb deletion caused by non-allelic homologous recombination between two LINE-1 elements in a patient with mesomelia-synostosis syndrome.2017

    • Author(s)
      Kohmoto T, Naruto T, Watanabe M, Fujita Y, Ujiro S, Okamoto N, Horikawa H, Masuda K, Imoto I.
    • Journal Title

      Am J Med Genet A

      Volume: 173 Issue: 4 Pages: 1082-1086

    • DOI

      10.1002/ajmg.a.38122

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Detection of 1p36 deletion by clinical exome-first diagnostic approach.2016

    • Author(s)
      Watanabe M, Horikawa H, et al.
    • Journal Title

      Hum Genome Var

      Volume: 3 Issue: 1

    • DOI

      10.1038/hgv.2016.6

    • Description
      in press
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel missense mutation of COL5A2 in a patient with Ehlers-Danlos syndrome.2016

    • Author(s)
      Watanabe M, Nakagawa R, Naruto T, Kohmoto T, Suga K, Goji A, Kagami S, Masuda K, Imoto I.
    • Journal Title

      Hum Genome Var

      Volume: 3 Issue: 1 Pages: 16030-16030

    • DOI

      10.1038/hgv.2016.30

    • NAID

      120006955874

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A novel frameshift mutation of CHD7 in a Japanese patient with CHARGE syndrome.2016

    • Author(s)
      Kohmoto T, Shono M, Naruto T, Watanabe M, Suga K, Nakagawa R, Kagami S, Masuda K, Imoto I.
    • Journal Title

      Hum Genome Var

      Volume: 3 Issue: 1

    • DOI

      10.1038/hgv.2016.4

    • NAID

      120006955871

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A novel COL11A1 missense mutation in siblings with non-ocular Stickler syndrome2016

    • Author(s)
      Kohmoto T, Tsuji A, Morita K, Naruto T, Masuda K, Kashimada K, Enomoto K, Morio T, Harada H, Imoto I
    • Journal Title

      Hum Genome Var

      Volume: 3 Issue: 1 Pages: 16003-16003

    • DOI

      10.1038/hgv.2016.3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel human mutation and CRISPR/Cas genome-edited mice reveal importance of C-terminal domain of MSX1 in tooth and palate development2016

    • Author(s)
      S.N.Mitsui, A.Yasue, K.Masuda, T.Naruto, Y.Minegishi, S.Oyadomari, S.Noji, I.Imoto, E.Tanaka
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 38398-38398

    • DOI

      10.1038/srep38398

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 成人領域のゲノム医療の社会実装が小児遺伝に与える影響と課題ーがんゲノム医療を中心にー2018

    • Author(s)
      井本逸勢
    • Organizer
      第40回日本小児遺伝学会学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Functional modification of TIA1 could be a potential therapeutic target for esophageal squamous cell carcinoma2017

    • Author(s)
      Masuda K, Hamada J, Fujita Y, Shoda K, Kohmoto T, Tange S, Imoto I
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of CPIG7.2 as a candidate for triple-negative breast cancer -associated gene through pan-cancer analysis2017

    • Author(s)
      Tange S, Masuda K, Kohmoto T, Tajima A, Imoto I
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of novel tumor-promoting gene, OEGC1, as a putative prognosticator for gastric cancer2017

    • Author(s)
      Kohmoto T, Masuda K, Shoda K, Tange S, Imoto I
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] RNA結合蛋白質MRF1による癌関連miRNA発現異常を介した癌促進機構2017

    • Author(s)
      井本逸勢、藤田悠司、河本知大、丹下正一朗、増田清士
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脂肪委縮症を伴う希少疾患の遺伝カウンセリングと診療科間連携に関する考察2017

    • Author(s)
      井本逸勢,宮本容子,吉田友紀子, 桑内敬子, 郷司彩, 島田亜紀, 小谷裕美子, 横田一郎
    • Organizer
      第41回日本遺伝カウンセリング学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The first Japanese patient of Mandibular hypoplasia, deafness, progeroid features, and lipodystrophy syndrome (MDPL) diagnosed via POLD1 mutation detection2017

    • Author(s)
      Imoto I, Kohmoto T, Naruto T, Goji A, Okada A, Yokota I, Kotani M, Shimada A, Miyamoto Y, Masuda K, Kagami S
    • Organizer
      American Society of Human Genetics 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA結合蛋白質TIA1の機能調節に着目した新規食道癌治療法の開発2017

    • Author(s)
      Masuda K, Kohmoto T, Hamada J, Shoda K, Fujita Y, Tange S, Saito M, Imoto I
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] MeCP2-e1 遺伝子変異が検出された典型的Rett症候群の男児例2017

    • Author(s)
      郷司彩,伊藤弘道,戸梶成美,河本知大,成戸卓也,森達夫,東田好広,増田清士,香美祥二,井本逸勢
    • Organizer
      日本人類遺伝学会第62回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The contribution of deleterious germ-line mutations of susceptibility2017

    • Author(s)
      Hirasawa A, Imoto I, Naruto T, Akahane T, Yamagami T, Susumu N, Tsuda H, Aoki D
    • Organizer
      American Association for Cancer Research Annual Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] がんのPrecision Medicineとがんゲノム医療2017

    • Author(s)
      井本逸勢
    • Organizer
      第15回日本乳癌学会近畿地方会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] がん・遺伝疾患の臨床ゲノム解析の現場2017

    • Author(s)
      井本逸勢
    • Organizer
      NGS現場の会第五回研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Frequent silencing of the candidate tumor suppressor TSLAC1 by promoter methylation in early stage lung adenocarcinoma.2016

    • Author(s)
      Kohmoto T, Kajiura K, Naruto T,Masuda K、Kondo K, Tangoku A, Imoto I.
    • Organizer
      The 66th Annual Meeting of the American Society of Human Genetics
    • Place of Presentation
      Vancouver (Canada)
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Long interspersed nuclear element-1 (LINE1)-mediated 8q13 microdeletion detected in a Japanese case with mesomelia-synostoses syndrome.2016

    • Author(s)
      Imoto I, Naruto T, Kohmoto T, Watanabe M, Ujiro S, Masuda K.
    • Organizer
      The 66th Annual Meeting of the American Society of Human Genetics
    • Place of Presentation
      Vancouver (Canada)
    • Year and Date
      2016-10-20
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] がん体細胞変異検出の特性からみた検査の考え方2016

    • Author(s)
      井本逸勢
    • Organizer
      第23回日本遺伝子診療学会大会
    • Place of Presentation
      イイノホール&カンファレンスセンター(東京都・千代田区)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 肺腺癌の早期からDNAメチル化により高頻度に発現抑制を受ける新規癌抑制遺伝子候補TSLAC1の同定2016

    • Author(s)
      成戸卓也、梶浦耕一郎、増田清士、近藤和也、丹黒章、井本逸勢
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] RNA結合蛋白質TIA1による食道癌進展機構の解明2016

    • Author(s)
      増田清士、濵田隼一、庄田勝俊、藤田悠司、井本逸勢
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規RNA結合蛋白質MRF1は癌関連microRNA発現以上を誘導し食道癌の進展を促進する2016

    • Author(s)
      藤田悠司、増田清士、井本逸勢
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
  • [Presentation] EBV関連胃癌患者における遊離DNAの有用性の検討2016

    • Author(s)
      庄田勝俊、市川大輔、藤田悠司、増田清士、濵田隼一、有田智洋、小西博貴、小松周平、塩崎敦、岡本和真、井本逸勢、大辻英吾
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
  • [Book] 婦人科腫瘍遺伝カウンセリングマニュアル2018

    • Author(s)
      関沢 明彦、佐村 修、四元淳子
    • Total Pages
      130
    • Publisher
      中外医学社
    • ISBN
      9784498060869
    • Related Report
      2017 Annual Research Report
  • [Book] 遺伝/ゲノム看護2018

    • Author(s)
      井ノ上逸朗、有森 直子、溝口満子
    • Total Pages
      260
    • Publisher
      医歯薬出版
    • ISBN
      9784263236994
    • Related Report
      2017 Annual Research Report
  • [Remarks] 徳島大学医学部人類遺伝学分野

    • URL

      http://jiniden.ait231.tokushima-u.ac.jp/web/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Remarks] 徳島大学医学部人類遺伝学分野

    • URL

      http://www.tokushima-u.ac.jp/med/culture/jinruiiden/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi