| Project/Area Number |
16K15620
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
米満 吉和 九州大学, 薬学研究院, 教授 (40315065)
沖 英次 九州大学, 大学病院, 助教 (70380392)
佐伯 浩司 九州大学, 大学病院, 助教 (80325448)
原田 結 九州大学, 薬学研究院, 助教 (00608507)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | cancer stem cell / niche / irinotecan / がん幹細胞 / ニッチ / 抗がん剤耐性 / 薬剤耐性 / 消化器がん |
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| Outline of Final Research Achievements |
To find and investigate stem cell niche for the maintenance of stem cells, colon cancer cell line PLR123 was treated with irinotecan and the remnant cells were sorted into SP and non-SP fractions, and analyze expression levels of mRNA and protein. In cell sorter, SP fraction increased after treatment with irinotecan. ABC transporter and cytokeratin RNAs were upregulated in cancer cells of SP fraction in the microarray. We confirmed the CK20 and BCRP (Breast Cancer Resistance Protein) expression with immunohistochemistry in the cell block of PLR123 irinotecan treated cell. We will elucided the existence of niche-like cells and identify the key molecule which needed to form the niche.
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