| Project/Area Number |
16K15633
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
荒井 健一 佐賀大学, 医学部, 特任助教 (40752960)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 組織工学 / 再生医療 / 心臓疾患外科学 / スキャフォールドフリー / バイオ３Dプリンタ / バイオ3Dプリンタ / ヒトiPS細胞由来心筋細胞 / スフェロイド / 心臓 / iPS細胞 |
|---|
| Outline of Final Research Achievements |
We developed a Bio-3D printer tissue engineering technology in which cells aggregated into spheroids can be printed onto a scaffold-free needle array according to the desired 3D design. In this study, we fabricated a dome shape cardiac construct and optimized a culture environment for that new form.
Firstly, we fabricated a dome shape construct using human neonatal dermal fibroblasts and studied the optimization of culture environment. As a result, a dome shape fibroblast construct could be removed from needle array and cultured in the improved culture method. The shape of constructs could be maintained onto needle array. Secondly, we fabricated dome shape constructs with human cardiac spheroids. Although desired dome shape of constructs was maintained after removing from needle array, the beating of the constructs was weak. In the next step, we plane to improve the culture environment of dome shape cardiac constructs by redesigning needle array.
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