| Project/Area Number |
16K15644
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
岡戸 晴生 (亀山 茂樹) 東京都医学総合研究所 (60221842)
目黒 玲子 新潟大学 (30251804)
戸田 春男 新潟医療福祉大学 (10217507)
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| Research Collaborator |
園田 真樹 新潟大学
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 笑い発作 / てんかん性脳症 / 視床下部 / 視床 / 光遺伝学 / チャネルロドプシン / 作業記憶 / 視床下部過誤腫 / 手綱核 / 視床背内側核 / ラット / 動物モデル / 前頭葉 / 脳神経外科学 / 脳・神経 |
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| Outline of Final Research Achievements |
We introduced ChR2 into lateral hypothalamus using AAV9 viral vectors, and photostimulated ChR2-expressing axonal terminals in the dorsomedial and lateral habenular nuclei in the thalamus at 80 Hz when the rats conducted a spatial working memory and control visual discrimination tasks. The pathway-specific photostimulation induced transient increases and late suppression of δ～β and γ powers, and induced working memory task-specific behavioral impairments when stimulation was applied during the cue-presenting period, but not during the delay period. These results demonstrated the feasibility of pathway-specific photostimulation in mimicking post-epileptic neural circuit changes, indicating that transient hyperactivation and subsequent suppression of the hypothalamus-dorsal thalamic pathway plays a role in cognitive impairments following gelastic seizures.
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